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A newly-isolated marine methanogen harbors a small cryptic plasmid

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Abstract

Of 21 recently isolated strains of methanococci, one was found to harbor a small, cryptic, low copy number plasmid. Reproducible recovery was achieved by alkaline lysis of cells pretreated with proteinase K in an osmotically stabilizing buffer. The plasmid was found to contain a singleAval site. No homology was detected between the plasmid and DNA from any of the other new strains or from five known species of methanococci.

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Authors and Affiliations

  1. Department of Microbiology, University of Illinois, 131 Burrill Hall, 407 S. Goodwin, 61801, Urbana, IL, USA

    Alvin G. Wood & Jordan Konisky

  2. Department of Microbiology, University of Georgia, 30602, Athens, GA, USA

    William B. Whitman

Authors
  1. Alvin G. Wood
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  2. William B. Whitman
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  3. Jordan Konisky
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Wood, A.G., Whitman, W.B. & Konisky, J. A newly-isolated marine methanogen harbors a small cryptic plasmid. Arch. Microbiol. 142, 259–261 (1985). https://doi.org/10.1007/BF00693400

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  • DOI: https://doi.org/10.1007/BF00693400

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