Abstract
The effect of carbon dioxide on pigment and membrane content in Synechococcus lividus was studied by depriving cells of CO2 and examining cell populations biochemically and by electron microscopy. After 120 h of CO2 deprivation, S. lividus lost all detectable chlorophyll a and C-phycocyanin. Such bleached cultures were “mustard yellow”, the result of approximately 1.8 times more carotenoid per cell than green control cultures.
Although cells from beached cultures appeared morphologically identical to control green cells when examined by light microscopy, electron microscopic examination revealed them to be devoid of detectable thylakoid membrane. Thylakoid membrane could not be recovered by physical isolation or revealed by freeze etching of bleached S. lividus. In addition, inclusion bodies characteristically found in S. lividus were also absent.
Reintroduction of CO2 into bleached cultures resulted in a rapid resynthesis of both chlorophyll a and C-phycocyanin. Electron microscopic examination of these regreening cultures revealed that thylakoid membrane was also rapidly resynthesized. Growth of regreened cultures did not occur until there was the synthesis of a full complement of chlorophyll a, C-phycocyanin, and thylakoid membrane.
A time course study of the cytological events occurring during bleaching and regreening is presented.
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References
Allen, M. M., Smith, A. J.: Nitrogen chlorosis in blue-green algae. Arch. Mikrobiol. 69, 114–120 (1969)
Bunn, C. R., Keele, B. B., Jr., Elkan, G. H.: A technique for improved thin-layer chromatography of phospholipids. J. Chromatog. 45, 326–328 (1969)
Carroll, K. K.: Quantitative estimation of peak areas in gas-liquid chromatography. Nature (Lond.) 191, 377–378 (1961)
Castenholz, R. W.: Laboratory culture of thermophilic cyanophytes. Schweiz. Z. Hydrologie 32, 538–551 (1970)
Dittmer, J. C., Lester, R. L.: A simple, specific spray for the detection of phospholipids on thin-layer chromatograms. J. Lipid Res. 5, 126–127 (1964)
Drawert, H.: Zellmorphologische und zellphsiologische Studien an Cyanophyceen. I. Mitteilung. Literaturübersicht und Versuche mit Oscillatoria borneti Zuckal. Planta (Berl.) 37, 161–209 (1949)
Edwards, M. R., Berns, D. S., Ghiorse, W. O., Holt, S. C.: Ultrastructure of the thermophilic blue-green alga, Synechococcus lividus Copeland. J. Phycol. 4, 283–298 (1968)
Edwards, M. R., Gantt, E.: Phycobilisomes of the thermophilic blue-green alga Synechococcus lividus. J. Cell Biol. 50, 896–900 (1971)
Eley, J. H.: Effect of carbon dioxide concentration on pigmentation in the blue-green alga Anacystis nidulans. Plant Cell. Physiol. 12, 311–316 (1971)
Folch, J., Lees, M., Sloane, S. G. H.: A simple method for the isolation and purification of total lipides from a nimal tissues. J. Biol. Chem. 226, 497–509 (1957)
Van Gorkom, H. J., Donze, M.: Localization of nitrogen fixation in Anabaena. Nature (Lond.) 234, 231–232 (1971)
Gregory, D. W., Pirie, B. J. S.: Wetting agents for biological electron microscopy. I. General considerations and negative staining. J. Microscopy 99, 251–265 (1973)
Hartree, E. F.: Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal. Biochem. 48, 422–427 (1972)
Holt, S. C., Edwards, M. R.: Fine structure of the thermophilic blue-green alga Synechococcus lividus Copeland. A study of the frozen-fractured-etched cells. Can. J. Microbiol. 18, 175–181 (1972).
Jacin, H., Mishkin, A. R.: Separation of carbohydrates on borate impregnated silica gel G plates. J. Chromatog. 18, 170–173 (1965)
Jones, L. W., Meyers, J.: Pigment variations in Anacystis nidulans induced by light of selected wavelengths. J. Phycol. 1, 7–14 (1965)
Kellenberger, E., Ryter, A., Sechaud, J.: Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J. Biophys. Biochem. Cytol. 4, 671–678 (1958)
Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.) 227, 680–685 (1970)
Lang, N. J., Simon, R. D., Wolk, C. P.: Correspondence of cyanophycin granules with structured granules in Anabaena cylindrica. Arch. Mikrobiol. 83, 313–320 (1972)
Luft, J. H.: Improvements in epoxy resin embedding methods. J. Biophys. Biochem. Cytol. 9, 409–414 (1961)
McKinney, G.: Absorption of light by chlorophyll solutions. J. Biol. Chem. 140, 315–322 (1941)
Moss, C. W., Lamberg, M. A., Merwin, W. H.: Comparison of rapid methods for analysis of bacterial fatty acids. Applied Microbiol. 28, 80–85 (1974)
Nichols, B. W.: Comparative lipid biochemistry of photosynthetic organisms. In: Phytochemical phylogeny (J. B. Harborne, ed.), pp. 105–118. London-New York: Academic Press 1970
Öquist, G.: Changes in pigment composition and photosynthesis induced by iron-deficiency in the blue-green alga Anacystis nidulans. Physiol. Plant. 25, 188–191 (1971)
Peary, J. A., Castenholz, R. W.: Temperature strains of a thermophilic blue-green alga. Nature (Lond.) 202, 720–721 (1964)
Poukka, R., Vasenius, L., Turpeinen, O.: Catalytic hdyrogenation of fatty acid methyl esters for gas-liquid chromatography. J. Lipid Res. 3, 128–129 (1962)
Prakash, G., Kumar, H. D.: Studies on sulfur-selenium antagonisms in blue-green algae. I. sulfur nutrition. Arch. Mikrobiol. 77, 196–202 (1971)
Reynolds, E. S.: The use of lead citrate at a high pH as an electronopaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)
Sjöström, M., Thornell, L.-E., Hellström, S.: An efficient standardized method of staining thin sections for electron microscopy. J. Ultrastructure Res. 42, 180–185 (1973)
Svedberg, T., Katsurai, T.: The molecular weights of phycocyan and phycoerythrin from Porphyra tenera and of phycocyan from Aphanizomenon flos-aquae. J. Amer. Chem. Soc. 51, 3573–3583 (1929)
de Vasconcelos, L., Fay, P.: Nitrogen metabolism and ultrastructure in Anabaena cylindrica. I. The effect of nitrogen starvation. Arch. Microbiol. 96, 271–279 (1974)
Vasil'eva, V. E., Levitin, M. G.: Effect of carbon dioxide starvation on certain blue-green algae. Sov. Plant Physiol. 21, 999–1002 (1974)
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Miller, L.S., Holt, S.C. Effect of carbon dioxide on pigment and membrane content in Synechococcus lividus . Arch. Microbiol. 115, 185–198 (1977). https://doi.org/10.1007/BF00406374
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DOI: https://doi.org/10.1007/BF00406374