Abstract
In this paper we report studies on photosynthetic formation of inorganic pyrophosphate (PPi) in three phototrophic bacteria. Formation of PPi was found in chromatophores from Rhodopseudomonas viridis but not in chromatophores from Rhodopseudomonas blastica and Rhodobacter capsulatus. The maximal rate of PPi synthesis in Rps. viridis was 0.15 μmol PPi formed/(min*μmol Bacteriochlorophyll) at 23°C. The synthesis of PPi was inhibited by electron transport inhibitors, uncouplers and fluoride, but was insensitive to oligomycin and venturicidin. The steady state rate of PPi synthesis under continuous illumination was about 15% of the steady-state rate of ATP synthesis. The synthesis of PPi after short light flashes was also studied. The yield of PPi after a single 1 ms flash was equivalent to approximately 1 μmol PPi/500 μmol Bacteriochlorophyll. In Rps. viridis chromatophores, PPi was also found to induce a membrane potential, which was sensitive to carbonyl cyanide p-trifluoromethoxyphenylhydrazone and NaF.
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Abbreviations
- BChl:
-
Bacteriochlorophyll
- F0F1-ATPase:
-
Membrane bound proton translocating ATP synthase
- FCCP:
-
Carbonyl cyanide p-trifluoromethoxyphenylhydrazone
- H+-PPase:
-
Membrane bound proton translocating PPi synthase
- TPP+ :
-
Tetraphenyl phosphonium ion
- TPB- :
-
Tetraphenyl boron ion
- ΔΨ:
-
Transmembrane electrical potential difference
References
Baltscheffsky H. and von Stedingk L.-V. (1966) Biochem. Biophys. Res. Commun. 22, 722–728.
Baltscheffsky H., von Stedingk L.-V., Heldt H.-W. and Klingenberg M. (1966) Science 153, 1120–1122.
Bose S.K., Gest H., and Ormerod J.G. (1961) J. Biol. Chem. 236, PC 13–14.
Casadio R., Venturoli G. and Melanderi B.A. (1981) Photobiochem. Photobiophys. 2, 245–253.
Clark A.J., Cotton N.P.J. and Jackson J.B. (1983) Biochim. Biophys. Acta 723, 440–453.
Clayton R.K. (1963) Biochim. Biophys. Acta 75, 312–323.
Clayton R.K. (1963) in Bacterial Photosynthesis (Gest H., San Pietro A., and Vernon L.P., eds.), p. 495–500, Antioch Press, Yellow Springs, Ohio.
Clayton R.K. (1966) Photochem. Photobiol. 5, 669–677.
Guillory R.J. and Fisher R.R. (1972) Biochem. J. 129, 471–481.
Jones O.T.G. and Saunders V.A. (1972) Biochim. Biophys. Acta 275, 427–436.
Kamo N., Muratsugu M., Hongoh R. and Kobatake Y. (1979) J. Membr. Biol. 49, 105–121.
Kerber N.L., Pucheu N.L. and Garcia A.F. (1977) FEBS Lett. 80, 49–52.
Knaff D.B. and Carr J.W. (1979) Arch. Biochem. Biophys. 193, 379–384.
Knobloch K. (1975). Z. Naturforsch. 30c, 342–348.
Nore B.F., Husain I., Nyrén P. and Baltscheffsky M. (1986) FEBS Lett. 200, 133–138.
Nyrén P., Hajnal K. and Baltscheffsky M. (1984) Biochim. Biophys. Acta 766, 630–635.
Nyrén P. and Lundin A. (1985) Anal. Biochem. 151, 504–509.
Nyrén P., Nore B.F. and Baltscheffsky M. (1986) Biochim. Biophys. Acta 851, 276–282.
Nyrén P., Nore B.F. and Baltscheffsky M. (1986) Photobiochem. Photobiophys. 11, 189–196.
Rathbun W.B. and Betlach V.M. (1969) Anal. Biochem. 28, 436–445.
Schwarm H.-M., Vigenschow H. and Knobloch K. (1986) Biol. Chem. Hoppe-Seyler 367, 127–133.
Shakhov Yu.A., Nyrén P. and Baltscheffsky M. (1982) FEBS Lett. 146, 177–180.
Sherman L.A. and Clayton R.K. (1972) FEBS Lett. 22, 127–132.
Strid Å., Karlsson I.-M. and Baltscheffsky M. (1987) FEBS Lett. 224, 348–352.
Strid Å., Nyrén P., Boork J. and Baltscheffsky M. (1986) FEBS Lett. 196, 337–340.
Zannoni D. (1984) Arch. Microbiol. 140, 15–20.
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Nore, B.F., Nyrén, P., Salih, G.F. et al. Photosynthetic formation of inorganic pyrophosphate in phototrophic bacteria. Photosynth Res 24, 75–80 (1990). https://doi.org/10.1007/BF00032646
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DOI: https://doi.org/10.1007/BF00032646