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Regulation of endoreduplication in maize (Zea mays L.) endosperm. Isolation of a novel B1-type cyclin and its quantitative analysis

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Abstract

To investigate the involvement of cyclin in mitotic and endoreduplicative cell cycle control, we have isolated a mitotic cyclin clone from a maize endosperm cDNA library. The deduced amino acid sequence of this clone identifies a novel B1-type cyclin with distinctly different sequence in regions with putative involvement in intracellular localization. This cyclin, designated Zeama;CycB1;3 (CycZme1), was shown by RNA gel blots and quantitative RT-PCR to be specific for tissues engaging in cell proliferation. It accumulated in metaphase-arrested cells and declined rapidly upon release into G1 phase. During the transition from mitosis to endoreduplication in maize endosperm, CycZme1 transcript declined precipitously while transcripts associated with S phase (histone-H3 and PCNA) and multiple phases of the cell cycle (Cdc2, α-tubulin) remained at moderate to high levels. We conclude that CycZme1 down-regulation is involved in the cellular transition to endoreduplication.

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  1. Yuejin Sun

    Present address: Pioneer Hi-Bred International, Johnston, Iowa, USA

Authors and Affiliations

  1. Department of Soil, Crop and Atmospheric Sciences, Cornell University, Ithaca, NY, 14853, USA

    Brian A. Flannigan & Tim L. Setter

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  1. Yuejin Sun
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Sun, Y., Flannigan, B.A. & Setter, T.L. Regulation of endoreduplication in maize (Zea mays L.) endosperm. Isolation of a novel B1-type cyclin and its quantitative analysis. Plant Mol Biol 41, 245–258 (1999). https://doi.org/10.1023/A:1006315625486

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