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Comparative sequence analysis of cis elements present in Glycine max L. leghemoglobin lba and lbc3 genes

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Abstract

The soybean leghemoglobin lba gene promoter sequence was determined and aligned with the promoter sequence of the soybean lbc3 gene from the same gene family. Five highly conserved regions were found. There are two large conserved regions, one of which overlaps the basic promoter while the other defines a minimal enhancer in the upstream positive elements. Within the minimal enhancer, an inverted repeat with similarity to the binding site of a yeast transcription factor, GCN4, was found. This particular repeat is conserved in the promoters of all functional soybean lb genes as well as in lb gene promoters from other legumes. This suggests that the inverted repeat is important for leghemoglobin gene expression.

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Author notes

  1. Qunxin She

    Present address: Department of Microbiology, The Technical University of Denmark, Building 221, DK-2800, Lyngby, Denmark

Authors and Affiliations

  1. Laboratory of Gene Expression, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10, DK-8000, Aarhus C, Denmark

    Qunxin She, Niels N. Sandal, Jens Stougaard & Kjeld A. Marcker

Authors
  1. Qunxin She
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  2. Niels N. Sandal
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  3. Jens Stougaard
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  4. Kjeld A. Marcker
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Additional information

The functional analysis of the soybean lba promoter and the conserved upstream positive elements of the lba and lbc3 genes are reported in a companion paper [13].

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She, Q., Sandal, N.N., Stougaard, J. et al. Comparative sequence analysis of cis elements present in Glycine max L. leghemoglobin lba and lbc3 genes. Plant Mol Biol 22, 931–935 (1993). https://doi.org/10.1007/BF00027380

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  • DOI: https://doi.org/10.1007/BF00027380

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