Abstract
WIS-2-1A, a 8624 bp insertion in the Glu-1A-2 locus of chromosome 1A of wheat, consists of two 1755 bp long terminal repeats enclosing a 5114 bp internal region. No long open reading frames could be found, but inspection of the predicted amino acid sequence showed regions with homology to retrotransposon structures, including a methionine tRNA initiator binding site, a nucleotide binding domain, a protease, an integrase and a polymerase. DNA replication errors have resulted in frame-shifts in the protein coding region, suggesting that retrotransposition of WIS-2-1A, if it occurs, must be mediated by trans-acting factors.
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Murphy, G.J.P., Lucas, H., Moore, G. et al. Sequence analysis of WIS-2-1A, a retrotransposon-like element from wheat. Plant Mol Biol 20, 991–995 (1992). https://doi.org/10.1007/BF00027169
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DOI: https://doi.org/10.1007/BF00027169