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A photosystem II polypeptide is encoded by an open reading frame co-transcribed with genes for cytochrome b-559 in wheat chloroplast DNA

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Abstract

The N-terminal amino acid sequence of a 3.2 kDa photosystem II polypeptide is shown to be identical to that of a polypeptide encoded by an open reading frame of 38 codons (orf38) in wheat chloroplast DNA. Orf38 is located just downstream of the psbE and psbF genes for the polypeptides of cytochrome b-559. Analysis of the transcription of this region of chloroplast DNA shows that psbE, psbF and orf38 are co-transcribed to give a 1.1 kb polycistronic transcript which also contains another open reading frame of 40 codons. The orf38 and orf40 products are hydrophobic polypeptides which are both predicted to span the thylakoid membrane once. Orf38 and orf40 are highly conserved, and map to similar locations adjacent to psbE and psbF, in all organisms from which this region of DNA has been sequenced. We propose that orf38 is named psbL.

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Authors and Affiliations

  1. Department of Botany, University of Cambridge, CB2 3EA, Cambridge, UK

    Andrew N. Webber, Sean M. Hird & John C. Gray

  2. Department of Biochemistry, University of Cambridge, CB2 3EA, Cambridge, UK

    Leonard C. Packman

  3. Institute of Plant Science Research, Maris Lane, Trumpington, CB2 2JB, Cambridge, UK

    Tristan A. Dyer

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  1. Andrew N. Webber
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  2. Sean M. Hird
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  3. Leonard C. Packman
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  4. Tristan A. Dyer
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  5. John C. Gray
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Webber, A.N., Hird, S.M., Packman, L.C. et al. A photosystem II polypeptide is encoded by an open reading frame co-transcribed with genes for cytochrome b-559 in wheat chloroplast DNA. Plant Mol Biol 12, 141–151 (1989). https://doi.org/10.1007/BF00020499

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  • DOI: https://doi.org/10.1007/BF00020499

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