Abstract
THE interdependence of various types of macromolecular synthesis in the cell has been demonstrated by the use of various antimetabolites. Synthesis of some ribonucleoproteins (RNP) in the nucleus is dependent on continued formation of deoxyribonucleic acid (DNA)1–3. One of these ribonucleoproteins is present in the nucleolus in the form of spherules called ‘nucleolini’3. When synthesis of DNA was inhibited by 5-fluorodeoxyuridine (FUDR), the nucleolini were at first enlarged and later disappeared. The pars amorpha of the nucleolus was not reduced during this process3. Further cytochemical observations and biochemical investigations of HeLa cells, grown as previously described3, and treated with 2 × 10−5 M FUDR are presented here.
Similar content being viewed by others
Article PDF
References
Paul, J., and Hagiwara, A., Biochim. Biophys. Acta, 61, 243 (1962).
Salzman, N. P., and Sebring, E. D., Biochim. Biophys. Acta, 61, 406 (1962).
Love, R., and Walsh, R. J., Nature, 197, 795 (1963).
Love, R., and Walsh, R. J., J. Histochem. Cytochem., 11, 188 (1963).
Newton, A., and Stoker, M. G. P., Virology, 5, 549 (1958).
Fleck, A., and Munro, H. N., Biochim. Biophys. Acta, 55, 571 (1962).
Burton, K., Biochem. J., 62, 315 (1956).
Kerr, S. E., and Seraidarian, K., J. Biol. Chem., 159, 211 (1945).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).
Love, R., J. Histochem. Cytochem. (in the press).
Love, R., Walsh, R. J., and Studzinski, G. P., Proc. Amer. Assoc. Cancer Res., 4, 40 (1963).
Reich, E., Franklin, R. M., Shatkin, A. J., and Tatum, E. L., Proc. U.S. Nat. Acad. Sci., 48, 1238 (1962).
Perry, R. P., Proc. U.S. Nat. Acad. Sci., 48, 2179 (1962).
Hurwitz, J., Furth, J. J., Malamy, M., and Alexander, M., Proc. U.S. Nat. Acad. Sci., 48, 1222 (1962).
Lieberman, I., Abrams, R., and Ove, P., J. Biol. Chem., 238, 2141 (1963).
Paul, J., and Struthers, M. G., Biochem. Biophys. Res. Commun., 11, 135 (1963).
Watson, J. D., Science, 140, 17 (1963).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
LOVE, R., CLARK, A. & STUDZINSKI, G. Cytochemical Investigations of Deoxyribonucleic Acid-mediated Control of Ribonucleoprotein Metabolism. Nature 203, 1384 (1964). https://doi.org/10.1038/2031384a0
Issue Date:
DOI: https://doi.org/10.1038/2031384a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.