Summary
The endocrine cells of the processus uncinatus in the dog pancreas were investigated with special reference to the formerly known F-cell. The F-cell was detected frequently in the periphery of pancreatic islets as well as among exocrine tissue. In both localizations the F-cell shows similar ultrastructural features. Membrane-bound irregularly shaped secretory granules of variable electron density were seen.
The cell possesses all features of an endocrine polypeptide secreting cell. Using the immunofluorescence and immunoperoxidase technique in the uncinate processus of the dog, we could reveal that the anti-sera against bovine pancreatic polypeptide (BPP) reacts with the cell which is localized at the same sites as the F-cell. We therefore concludes that the pancreatic F-cell is identical to the pancreatic polypeptide-producing cell. The other endocrine cell types of the dog pancreas are glucagon-producing A-cells, insulin-producing B-cells, and somatostatin-producing D-cells, as well as serotonin-producing EC-cells which are regularly present in the dog pancreatics islets and also scattered among exocrine tissue and the duct epithelial cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baum, J., Simons, B.E., Unger, R.H., Madison, L.L.: Localization of glucagon in the alpha-cells in the pancreatic islets by immunofluorescent technics. Diabetes 11, 371–374 (1962)
Bencosme, S.A., Liepa, E.: Regional differences of the pancreatic islets. Endocrinology 57, 588–593 (1955)
Caramia, F., Munger, B.L., Lacy, P.E.: The ultrastructural basis for the identification of cell types in the pancreatic islets. I. Guinea pig. Z. Zellforsch. 67, 533–546 (1965)
Deconinck, J.F., Van Assche, F.A., Potvliege, P.R., Gepts, W.: The ultrastructure of the human pancreatic islets. II. The islets of neonates. Diabetologia 8, 326–333 (1972)
Forssmann, A.: The ultrastructure of the cell types in the endocrine pancreas of the horse. Cell Tiss. Res. 167, 179–195 (1976)
Forssmann, W.G., Grube, D.: Studien über die endokrinen Zellen des Magendarmtraktes. I. Histochemische, fluoreszenzmikroskopische und ultrastrukturelle Untersuchung an Katze und Ratte. Z. Zellforsch. 140, 535–550 (1973)
Forssmann, W.G., Helmstaedter, V., Chance, R.E.: Ultrastructural and immunohistochemical demonstration of pancreatic polypeptide containing F-cells in the stomach, upper duodenum and pancreas of Tupaia belangeri. Cell Tiss. Res. (in press)
Forssmann, W.G. Ito, S., Weihe, E., Aoki, A., Dym, M., Fawcett, D.W.: An improved perfusion fixation method for the male genital tract. Anat. Rec. (submitted for publication)
Hazelwood, R.L., Turner, S.D., Kimmel, J.R., Pollock, H.G.: Spectrum effects of a new polypeptide (third hormone?) isolated from the chicken pancreas. Gen. comp. Endocr. 21, 485–497 (1973)
Helmstaedter, V., Feurle, G.E., Forssmann, W.G.: Glucagon-somatostatin cell and gastrin-somatostatin cell relationship in the stomach of a monkey. Cell Tiss. Res. (in press, 1976)
Helmstaedter, V., Feurle, G.E., Forssmann, W.G.: Insulin, glucagon and somatostatin immunoreactive endocrine cells in equine pancreas. Cell Tiss. Res. (in press)
Hökfelt, T., Effendic, S., Hellerström, C., Johansson, O., Luft, R., Arimura, A.: Cellular localisation of somatostatin in endocrine-like cells and neurons of the rat with special references to the A1-cell of the pancreatic islets and to the hypothalamus. Acta endocr. (Kbh.), Suppl. 200, 5–41 (1975)
Jirásek, K., Kubés, L.: A new endocrine cell type of the human pancreatic islets. Experientia (Basel) 28, 966–967 (1972)
Kimmel, J.R., Pollock, H.G., Hazelwood, R.L.: Isolation and characterization of chicken insulin. Endocrinology 83, 1323–1330 (1968)
Lacy, P.E., Davies, J.: Preliminary studies on the demonstration of insulin in the islets by the fluorescent antibody technic. Diabetes 6, 354–356 (1957)
Langslow, D.R., Kimmel, J.R., Pollock, H.G.: Studies on the distribution of a new avian pancreatic polypeptide and insulin among birds, reptiles, amphibians and mammals. Endocrinology 93, 558–565 (1973)
Larsson, L.-I., Sundler, F., Hakanson, R.: Immunohistochemical localization of human pancreatic polypeptide (HPP) to a population of islet cells. Cell Tis. Res. 156, 167–171 (1975)
Larsson, L.-I., Sundler, F., Hakanson, R., Pollock, H.G., Kimmel, J.R.: Localization of APP, a postulated new hormone, to a pancreatic endocrine cell type. Histochemistry 42, 377–382 (1974)
Lin, T.M., Chance, R.E.: Candidate hormones of the gut. VI. Bovine pancreatic polypeptide (BPP) and avian pancreatic polypeptide (APP). Gastroenterology 67, 737–738 (1974)
Misugi, K., Misugi, N., Satos, J., Smith, B.: The pancreatic islet of infants with severe hypoglycemia. Arch. Path. 89, 208–220 (1970)
Munger, B.L., Caramia, F., Lacy, P.E.: The ultrastructural basis for the identification of cell types in the pancreatic islets. II: Rabbit, dog and oppossum. Z. Zellforsch 67, 776–798 (1965)
Orci, L., Unger, R.H.: Functional subdivision of islets of Langerhans and possible role of D-cells. Lancet 1975II, 1243–1244
Pearse, A.G.E., Polak, J.M., Adams, C., Kendall, P.A.: Diethylpyrocarbonate, a vapour-phase fixative for immunofluorescence studies on polypeptide hormones. Histochem. J. 6, 347–352 (1974)
Polak, J.M., Adrian, T.E., Bryant, M.G., Bloom, S.R., Heitz, Ph., Pearse, A.G.E.: Pancreatic polypeptide in insulinomas gastrinomas, vipomas, and glucagonomas. Lancet 1976II, 328–330
Rufener, C., Amherdt, M., Dubois, M.P., Orci, L.: Ultrastructural immunocytochemical localization of somatostatin in rat pancreatic monolayer culture. J. Histochem. Cytochem. 23, 866–869 (1975)
Vassallo, G., Solcia, E., Bussolati, G., Polak, J.M., Pearse, A.G.E.: Non-G-cell gastrinproducing tumors of the pancreas. Virchows Arch. Abt. B 11, 66–79 (1972)
Author information
Authors and Affiliations
Additional information
We are indebted to Mrs. B. Brühl, M. Wagner and Ch. Müller for their technical assistance and Mrs. R. Botz for preparing the manuscript
Rights and permissions
About this article
Cite this article
Forssmann, W.G., Helmstaedter, V., Metz, J. et al. The identification of the F-cell in the dog pancreas as the pancreatic polypeptide producing cell. Histochemistry 50, 281–290 (1977). https://doi.org/10.1007/BF00507121
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00507121