Journal Description
Animals
Animals
is an international, peer-reviewed, open access journal devoted entirely to animals, including zoology and veterinary sciences, published semimonthly online by MDPI. The World Association of Zoos and Aquariums (WAZA), European College of Animal Welfare and Behavioural Medicine (ECAWBM), and Federation of European Laboratory Animal Science Associations (FELASA) are affiliated with Animals and their members receive a discount on the article processing charges.
- Open Access— free for readers, with article processing charges (APC) paid by authors or their institutions.
- High Visibility: indexed within Scopus, SCIE (Web of Science), PubMed, PMC, Embase, PubAg, AGRIS, Animal Science Database, CAB Abstracts, and other databases.
- Journal Rank: JCR - Q1 (Veterinary Sciences) / CiteScore - Q1 (General Veterinary)
- Rapid Publication: manuscripts are peer-reviewed and a first decision is provided to authors approximately 18.1 days after submission; acceptance to publication is undertaken in 2.5 days (median values for papers published in this journal in the second half of 2023).
- Recognition of Reviewers: reviewers who provide timely, thorough peer-review reports receive vouchers entitling them to a discount on the APC of their next publication in any MDPI journal, in appreciation of the work done.
- Companion journals for Animals include: Birds, Ruminants and Zoonotic Diseases.
Impact Factor:
3.0 (2022);
5-Year Impact Factor:
3.2 (2022)
Latest Articles
Automatic Identification of Pangolin Behavior Using Deep Learning Based on Temporal Relative Attention Mechanism
Animals 2024, 14(7), 1032; https://doi.org/10.3390/ani14071032 (registering DOI) - 28 Mar 2024
Abstract
With declining populations in the wild, captive rescue and breeding have become one of the most important ways to protect pangolins from extinction. At present, the success rate of artificial breeding is low, due to the insufficient understanding of the breeding behavior characteristics
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With declining populations in the wild, captive rescue and breeding have become one of the most important ways to protect pangolins from extinction. At present, the success rate of artificial breeding is low, due to the insufficient understanding of the breeding behavior characteristics of pangolins. The automatic recognition method based on machine vision not only monitors for 24 h but also reduces the stress response of pangolins. This paper aimed to establish a temporal relation and attention mechanism network (Pangolin breeding attention and transfer network, PBATn) to monitor and recognize pangolin behaviors, including breeding and daily behavior. There were 11,476 videos including breeding behavior and daily behavior that were divided into training, validation, and test sets. For the training set and validation set, the PBATn network model had an accuracy of 98.95% and 96.11%, and a loss function value of 0.1531 and 0.1852. The model is suitable for a 2.40 m × 2.20 m (length × width) pangolin cage area, with a nest box measuring 40 cm × 30 cm × 30 cm (length × width × height) positioned either on the left or right side inside the cage. A spherical night-vision monitoring camera was installed on the cage wall at a height of 2.50 m above the ground. For the test set, the mean Average Precision (mAP), average accuracy, average recall, average specificity, and average F1 score were found to be higher than SlowFast, X3D, TANet, TSN, etc., with values of 97.50%, 99.17%, 97.55%, 99.53%, and 97.48%, respectively. The recognition accuracies of PBATn were 94.00% and 98.50% for the chasing and mounting breeding behaviors, respectively. The results showed that PBATn outperformed the baseline methods in all aspects. This study shows that the deep learning system can accurately observe pangolin breeding behavior and it will be useful for analyzing the behavior of these animals.
Full article
(This article belongs to the Section Wildlife)
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Exploring the Importance of Environmental Complexity for Newly Hatched Zebrafish
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Maria Santacà, Elia Gatto, Marco Dadda, Matteo Bruzzone, Marco Dal Maschio and Angelo Bisazza
Animals 2024, 14(7), 1031; https://doi.org/10.3390/ani14071031 (registering DOI) - 28 Mar 2024
Abstract
The effects of an early impoverished social or physical environment on vertebrate neural development and cognition has been known for decades. While existing studies have focused on the long-term effects, measuring adult cognitive phenotypes, studies on the effects of environmental complexity on the
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The effects of an early impoverished social or physical environment on vertebrate neural development and cognition has been known for decades. While existing studies have focused on the long-term effects, measuring adult cognitive phenotypes, studies on the effects of environmental complexity on the early stages of development are lacking. Zebrafish (Danio rerio) hatchlings are assumed to have minimal interaction with their environment and are routinely reared in small, bare containers. To investigate the effects of being raised under such conditions on development of behaviour and cognition, hatchlings housed for 10 days in either an enriched or a standard environment underwent two cognitive tasks. The results were mixed. Subjects of the two treatments did not differ in performance when required to discriminate two areas. Conversely, we found a significant effect in a number discrimination task, with subjects from impoverished condition performing significantly worse. In both experiments, larvae reared in impoverished environment showed a reduced locomotor activity. Given the effects that enrichment appears to exert on larvae, a third experiment explored whether hatchlings exhibit a spontaneous preference for more complex environments. When offered a choice between a bare setting and one with objects of different shapes and colors, larvae spent over 70% of time in the enriched sector. Deepening these effects of an early impoverished environment on cognitive development is crucial for the welfare of captive zebrafish populations and for enhancing the quality and reliability of studies on larval zebrafish.
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(This article belongs to the Section Aquatic Animals)
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Morphometric Evaluation of Thoracolumbar Spinal Canal and Cord by Magnetic Resonance Imaging in Normal Small-Breed Dogs
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Gabchol Choi, Myungryul Yang, Seungweon Yang, Sungbeen Park, Suyoung Heo and Namsoo Kim
Animals 2024, 14(7), 1030; https://doi.org/10.3390/ani14071030 (registering DOI) - 28 Mar 2024
Abstract
Thoracolumbar intervertebral disc disease (IVDD) is the most common cause of spinal injury in dogs. MRI has been considered the gold standard for neurologic diagnosis, but studies focusing on the thoracolumbar spinal canal and spinal cord using MRI in small-breed dogs are limited.
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Thoracolumbar intervertebral disc disease (IVDD) is the most common cause of spinal injury in dogs. MRI has been considered the gold standard for neurologic diagnosis, but studies focusing on the thoracolumbar spinal canal and spinal cord using MRI in small-breed dogs are limited. Therefore, this study aimed to establish an MRI reference range for the spinal cord and canal measurements (height, width, cord-to-canal ratio of height, width, cross-sectional area (CSA)) of each intervertebral disc level from T11 to L5 (total of seven levels) on transverse T2-weighted images in normal small-breed dogs. We hypothesized that the spinal cord and spinal canal measurements might vary according to the body weight and age. The width and height of the spinal cord and canal increased as the body weight increased at all levels (p < 0.05). The cord-to-canal ratio of the width showed a negative correlation to the body weight at all levels. The cord-to-canal ratio of the height did not show any correlation to the body weight at all levels. All measurements (height, width, cord-to-canal ratio of height, width, CSA) did not show any statistical correlation between the groups subdivided by age. These measurements could serve as a morphometric baseline for thoracolumbar spinal diseases and clinical research in small-breed dogs.
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(This article belongs to the Section Veterinary Clinical Studies)
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Open AccessArticle
Diphyllobothrium sp. and Other Parasites of Migrating and Rare Fish Species in the Southern Baltic Sea and Coastal Waters, Poland
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Izabella Rząd, Beata Więcaszek, Angelika Linowska, Agata Korzelecka-Orkisz and Ewa Dzika
Animals 2024, 14(7), 1029; https://doi.org/10.3390/ani14071029 (registering DOI) - 28 Mar 2024
Abstract
The present study is the first to analyse the parasite fauna of sole Solea solea, dab Limanda limanda, hake Merluccius merluccius, whiting Merlangius merlangus, and plaice Pleuronectes platessa in the Pomeranian Bay, as well as saithe Pollachius virens from
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The present study is the first to analyse the parasite fauna of sole Solea solea, dab Limanda limanda, hake Merluccius merluccius, whiting Merlangius merlangus, and plaice Pleuronectes platessa in the Pomeranian Bay, as well as saithe Pollachius virens from the Szczecin Lagoon (Poland). The aim of this study was to determine the occurrence of parasites in migrating and rare fish in the Pomeranian Bay and the Szczecin Lagoon and to determine the composition of the diet of these fish. The fish for analysis were obtained in the years 2010–2019. The typical marine nematode Capillaria (Procapillaria) gracilis, rarely recorded in Poland, was found, in addition to the following parasites: Ichthyophonus hoferi, Trichodina jadranica, Diphyllobothrium sp., Dichelyne (Cucullanellus) minutus, Raphidascaris acus, Anisakis simplex, Contracaecum osculatum, Hysterothylacium aduncum, Pseudoterranova decipiens, and Echinorhynchus gadi. Because the fish species analysed in the study are not typically present in the Baltic (with the exception of plaice), and because we do not know how long they feed while they are in the Baltic, we cannot be certain which parasites they acquired in the water bodies analysed in the study and which were introduced during the migration of fish. Although these fish are outside of their normal geographic range (except for plaice), in the new environment, there were enough suitable intermediate hosts for the parasites of these fish to complete their life cycle and survive.
Full article
(This article belongs to the Special Issue Diagnosis, Evaluation, and Management of Wildlife, Exotic and Zoo Animals Disease)
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Development of a Predictive Model for Iron Levels in Bovine Muscle Tissue Using Hair as a Predictor
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Kirill Narozhnykh
Animals 2024, 14(7), 1028; https://doi.org/10.3390/ani14071028 (registering DOI) - 28 Mar 2024
Abstract
The assessment of iron levels in cattle muscle tissue is crucial for livestock management because it influences both animal health and meat quality, key factors in sustainable development. This study aimed to develop an optimal model for noninvasively predicting the iron content in
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The assessment of iron levels in cattle muscle tissue is crucial for livestock management because it influences both animal health and meat quality, key factors in sustainable development. This study aimed to develop an optimal model for noninvasively predicting the iron content in Hereford cattle muscle tissue, contributing to a comprehensive understanding of the animals’ elemental status. The research involved the atomic absorption analysis of muscle tissue and hair samples from cattle. A regression model was constructed using the least squares method to identify the most effective approach. These findings have ecological applications, aiding in evaluating environmental health and establishing acceptable iron thresholds for animals. The proposed mathematical model utilizing biomarkers (levels of Mg, K, Fe, Al, Cr in hair) will allow for the assessment of iron levels in cattle muscle tissue throughout the period of productive use, with the possibility of adjustment and tracking the changes in elemental status over time. The utilization of the developed method will enable the diagnosis of animal elementosis and assessment of the iron level burden. Subsequently, this will allow for the improvement of the qualitative characteristics of the final product. Thus, the obtained data contribute to fundamental knowledge regarding the content and variability of iron levels in the muscle tissue of cattle.
Full article
(This article belongs to the Special Issue Role of Trace Element in Animal Health and Metabolic: Second Edition)
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Open AccessArticle
Detection of Protein Biomarkers Relevant to Sperm Characteristics and Fertility in Semen in Three Wild Felidae: The Flat-Headed Cat (Prionailurus planiceps), Fishing Cat (Prionailurus viverrinus), and Asiatic Golden Cat (Catopuma temminckii)
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Manita Wittayarat, Supalak Kiatsomboon, Navapol Kupthammasan, Wanlaya Tipkantha, Surasak Yimprasert, Ampika Thongphakdee and Saritvich Panyaboriban
Animals 2024, 14(7), 1027; https://doi.org/10.3390/ani14071027 (registering DOI) - 28 Mar 2024
Abstract
Effective wild cat conservation programs with assisted reproductive technologies are being developed in different parts of the world. The flat-headed cat, fishing cat, and Asiatic golden cat are three species among nine wild Felidae in Thailand that are in need of urgent conservation
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Effective wild cat conservation programs with assisted reproductive technologies are being developed in different parts of the world. The flat-headed cat, fishing cat, and Asiatic golden cat are three species among nine wild Felidae in Thailand that are in need of urgent conservation efforts. Here, we assessed routine sperm characteristics and we report the detection of protein biomarkers related to the fertilization process, IZUMO1 and the CRISP family, and apoptotic markers, active or cleaved caspase-3, in semen samples collected from these wild cats. IZUMO1 was located in the equatorial segment of the sperm head, which is the region involved in gamete interaction. The highest levels of IZUMO1 were found in both the sperm pellet and the seminal plasma of the flat-headed cat, as determined by immunoblotting. CRISP2, a sperm–egg fusion assisting protein, and CRISP3 were found in both the sperm pellet and the seminal plasma, and the highest levels were observed in the fishing cat. Positive correlations between certain semen parameters and IZUMO1, CRISP2, and CRISP3 expression were also demonstrated. Cleaved caspase-3 was found in all sperm samples in all three species and was associated with an increase in DNA fragmentation and a decrease in certain semen characteristics such as motility, viability, and intact acrosomes. Our results suggest that the analysis of IZUMO1, the CRISP family, and cleaved caspase-3, along with the routine sperm characteristics, may allow for better success in breeding management in wild Felidae, particularly in the flat-headed cat and the fishing cat.
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(This article belongs to the Section Animal Reproduction)
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Effect of Dietary Vitamin C Supplementation on Growth Performance and Biochemical Parameters in Grower Walleye Pollock, Gadus chalcogrammus
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Ki Wook Lee, Hae Kyun Yoo, So-Sun Kim, Gyeong Sik Han, Min Min Jung and Hee Sung Kim
Animals 2024, 14(7), 1026; https://doi.org/10.3390/ani14071026 (registering DOI) - 28 Mar 2024
Abstract
The optimal dietary vitamin C (VC) levels for walleye pollock (Gadus chalcogrammus) remain undefined. This study aimed to assess the effect of dietary VC levels on the growth performance and biochemical parameters of grower walleye pollock and determine the optimal VC
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The optimal dietary vitamin C (VC) levels for walleye pollock (Gadus chalcogrammus) remain undefined. This study aimed to assess the effect of dietary VC levels on the growth performance and biochemical parameters of grower walleye pollock and determine the optimal VC level for their diet. Six experimental diets (VC0, VC1, VC3, VC5, VC7, and VC10) with VC levels of 3.24, 21.92, 63.31, 101.42, 145.46, and 202.51 mg kg−1 diet, respectively, were fed to fish (initial mean weight: 173.5 ± 0.31 g) for 8 weeks. At the end of the feeding trial, fish fed the VC7 and VC10 diets exhibited significantly higher growth (final body weight, weight gain, and specific growth rate) and improved feed utilization (feed efficiency and protein efficiency ratio) compared with fish fed the VC0 diet (p < 0.05). The VC3–VC10 diets significantly reduced plasma superoxide dismutase (SOD) activity (p < 0.05). Compared with the VC0 group, fish fed the VC7 and VC10 diets showed significantly elevated growth hormone and insulin-like growth factor-1 levels in plasma (p < 0.05). In conclusion, dietary VC supplementation in walleye pollock improved growth performance and SOD activity. Moreover, broken-line analysis on weight gain indicated that the optimal dietary VC level for grower walleye pollock was approximately 156.42 mg kg−1 diet.
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(This article belongs to the Section Aquatic Animals)
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Is It Possible to Mitigate Fear of Fireworks in Dogs? A Study on the Behavioural and Physiological Effects of a Psychoactive Supplement
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Daniela Ramos, Karina V. B. Yazbek, Amanda C. Brito, Barbara Georgetti, Luisa M. L. Dutra, Fabiola O. P. Leme and Angélica S. Vasconcellos
Animals 2024, 14(7), 1025; https://doi.org/10.3390/ani14071025 (registering DOI) - 28 Mar 2024
Abstract
Canine fear of fireworks is a common problem worldwide, with serious implications for the welfare of both dogs and their owners. Therapies for the problem are available, and herbal and nutraceutical agents are increasingly suggested by professionals; nonetheless, studies on their real efficacy
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Canine fear of fireworks is a common problem worldwide, with serious implications for the welfare of both dogs and their owners. Therapies for the problem are available, and herbal and nutraceutical agents are increasingly suggested by professionals; nonetheless, studies on their real efficacy in reducing firework fear are lacking. In a randomised, double-blinded, placebo-controlled study, 44 dogs (25 in the “supplement” group and 19 in the “placebo” group) completed a long-term continuous treatment with either a supplement made of tryptophan, valerian, and passiflora or a placebo, including two real exposures to fireworks (on 2020 Christmas and 2021 New Years’ Eve, after 42 and 48 days of treatment, respectively). Owners of both groups received the same general environmental management and food/toy offering recommendations for trying with their dogs on those nights. Behavioural (measured by LSSS—Lincoln Sound Sensitivity Scale and PANAS—Positive and Negative Activation scale, as rated by the owners) and stress (measured via salivary cortisol measures) reactions were evaluated. Significantly greater fear decrease (LSSS) was recorded in the “supplement” dogs, as compared to the “placebo” group. Cortisol dosages on New Year’s Eve (“noisy” night) were in line with behavioural results; “supplement” dogs showed a smaller increase in the stress response from 22:30 to 00:30 h on New Year’s Eve and a greater decrease in their stress response from 02:30 h to 10:30 h on New Year’s Day compared to “placebo” dogs. Smaller cortisol levels were also shown by “supplement” dogs than “placebo” dogs on a controlled “quiet night” (27th December). Owners’ rates on PANAS remained stable during the whole period of therapy for both groups. The evaluated supplement, a combination of tryptophan, valerian, and passiflora, showed satisfactory results and rare side effects when treating dogs fearful of fireworks.
Full article
(This article belongs to the Special Issue Management of Stress Impact on Domestic Animals Physiology and Welfare)
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Potential Implications of Acid-Sensing Ion Channels ASIC2 and ASIC4 in Gonadal Differentiation of Dicentrarchus labrax Subjected to Water Temperature Increase during Gonadal Development
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Kamel Mhalhel, Rosaria Arena, Maria Rizzo, Giuseppe Piccione, Marialuisa Aragona, Maria Levanti, Francesca Aragona and Francesca Arfuso
Animals 2024, 14(7), 1024; https://doi.org/10.3390/ani14071024 - 27 Mar 2024
Abstract
In this study, the expression and implication of acid-sensing ion channels 2 and 4 (ASIC2 and ASIC4) in the gonadal sex differentiation of Dicentrarchus labrax (D. labrax), subjected to increasing water temperatures during gonadal development, were evaluated. Two groups were selected:
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In this study, the expression and implication of acid-sensing ion channels 2 and 4 (ASIC2 and ASIC4) in the gonadal sex differentiation of Dicentrarchus labrax (D. labrax), subjected to increasing water temperatures during gonadal development, were evaluated. Two groups were selected: a control group (CG), in which the average water temperature was maintained at 15 °C and increased to 20 °C in 20 days until weaning; and an experimental group (EG), in which the water temperature was retained at 15 °C for 60 days; thereafter, the temperature was increased daily by 0.5 °C until it reached 20 °C up to the weaning time. Ten fish from the CG and 13 fish from the EG were sampled randomly on the 335th day after hatching (dph). A higher percentage of gonad differentiation in ovaries rather than in testes was observed in the EG compared to the CG (p = 0.01). ASIC2 and ASIC4 were detected for the first time in D. labrax ovaries by indirect immunofluorescence. Both ASIC2 and ASIC4 were expressed in previtellogenic oocytes of ovaries and in scattered cells within some testes, and were most likely intratesticular previtellogenic oocytes in both the CG and EG groups. The CG group showed a higher expression of ASIC4 than the EG cohort (p < 0.05). The results gathered in this study revealed the capacity of water temperature to influence both gonadal differentiation and growth in this gonochoristic fish species, and suggests the possible role of ASIC2 and ASIC4 in gonad differentiation and gamete development in D. labrax.
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(This article belongs to the Special Issue Sexual Maturation and Spawning in Fish)
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Dogs in Lithuania from the 12th to 18th C AD: Diet and Health According to Stable Isotope, Zooarchaeological, and Historical Data
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Giedrė Piličiauskienė, Raminta Skipitytė, Viktorija Micelicaitė and Povilas Blaževičius
Animals 2024, 14(7), 1023; https://doi.org/10.3390/ani14071023 - 27 Mar 2024
Abstract
This article presents the results of research that focused on the nutrition and related health issues of medieval and early modern dogs found in the territory of present-day Lithuania. In this study, we present bone collagen carbon (δ13C) and nitrogen (δ
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This article presents the results of research that focused on the nutrition and related health issues of medieval and early modern dogs found in the territory of present-day Lithuania. In this study, we present bone collagen carbon (δ13C) and nitrogen (δ15N) isotope ratios for seventy-five dogs recovered from seven sites which were dated back to the between the 12th and 18th C AD. In addition, by studying the remains of almost 200 dogs, we were able to estimate changes in the sizes and morphotypes of canines across over 600 years. On the basis of stable isotope and historical data, as well as the osteometric analysis, we discuss the dietary patterns of different sizes and types of dogs from the sites related to different social strata and time periods. The results of our study demonstrate that the size, type, diet, and health of canines from different time periods and sociocultural environments varied. Overall, carbon isotopic signals indicate that dogs’ diets were based on C3 plant environment foods (cereals and animals), while freshwater fish was more important for some individuals in coastal Klaipėda/Memelburg Castle. The stable isotope analysis supported the historical records, indicating that cereals were highly important in the diet of elite dogs. Meanwhile, urban dogs had a different nutrition. In the Middle Ages, the consumption of plant-based foods was likely higher compared to the early modern period. Our study also revealed that the diets of dogs did not correlate with individual size. Compared to pigs, dogs had a higher intake of animal foods in their diet. In general, the nutrition of the studied canines was similar to that of the rural human population of the same period.
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(This article belongs to the Special Issue Zooarchaeology: The History of Animal-Human Relations in the Natural and Cultural Environment)
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Open AccessSystematic Review
Toxocara cati Infection in Cats (Felis catus): A Systematic Review and Meta-Analysis
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Jorge Luis Bonilla-Aldana, Alba Cristina Espinosa-Nuñez, D. Katterine Bonilla-Aldana and Alfonso J. Rodriguez-Morales
Animals 2024, 14(7), 1022; https://doi.org/10.3390/ani14071022 - 27 Mar 2024
Abstract
Introduction: Toxocariasis is an infection caused in canines, felines, humans, and other vertebrates by species of the genus Toxocara, such as T. canis and T. cati. The embryonated eggs of these parasites are the primary means of acquiring the infection for
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Introduction: Toxocariasis is an infection caused in canines, felines, humans, and other vertebrates by species of the genus Toxocara, such as T. canis and T. cati. The embryonated eggs of these parasites are the primary means of acquiring the infection for both definitive hosts, dogs and cats, respectively, and for intermediates, such as humans and other vertebrates. When deposited on park soils, environmental contamination becomes a risk to environmental, human, and animal health. Objective: To determine the global prevalence of Toxocara cati in cats (Felis catus). Methods: A systematic review of the literature was carried out in six databases (Scopus, PubMed, ScienceDirect, SciELO and Google Scholar) to evaluate the global prevalence of Toxocara cati in cats, defined by coproparasitological, histological, and molecular techniques. A meta-analysis was performed using a random effects model to calculate pooled prevalence and 95% confidence intervals (95% CI). A two-tailed 5% alpha level was used for hypothesis testing. Results: Two hundred and eighty-nine studies were included. The global pooled prevalence of Toxocara cati in cats using coproparasitological methods was 17.0% (95.0% CI: 16.2–17.8%). In the subgroup analysis according to country, Nepal had the highest prevalence of T. cati infection (94.4%; 95% CI 89.7–99.2%). The pooled prevalence of T. cati infection by PCR in four studies was 4.9% (95.0% CI: 1.9–7.9%). Conclusions: This systematic review underscores the need for preventive action against toxocariasis due to its widespread prevalence. The interplay between animal and human health should be emphasised, necessitating measures like deworming cats, hygiene practices, and public education to mitigate risks. Safeguarding feline health can also reduce human transmission, benefiting both species.
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(This article belongs to the Special Issue Vector-Borne and Zoonotic Diseases in Dogs and Cats)
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A Model for Consumer Acceptance of Insect-Based Dog Foods among Adult UK Dog Owners
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Joanne Pinney and Montserrat Costa-Font
Animals 2024, 14(7), 1021; https://doi.org/10.3390/ani14071021 - 27 Mar 2024
Abstract
The use of alternative proteins is becoming more common in pet feed, and insect-based dog foods (IBDFs) are becoming more widely available. However, little research has been conducted to date in respect of the drivers for consumers’ acceptance of IBDF. This study aimed
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The use of alternative proteins is becoming more common in pet feed, and insect-based dog foods (IBDFs) are becoming more widely available. However, little research has been conducted to date in respect of the drivers for consumers’ acceptance of IBDF. This study aimed to investigate the acceptance of IBDF among adult UK dog owners and the factors influencing the decision to try and buy such products. A theoretical model was developed following a review of the existing literature. An online survey of 280 participants was carried out and the results were analysed using structural equation modelling (SEM) to test the theoretical model. The following constructs all had a significant impact on attitudes towards IBDF and/or intentions to try and buy IBDF: food preferences for animal welfare, health and environment; attitudes towards uses of animals; beliefs about insect sentience; disgust; perceptions of benefits and risks; and social norms. Social norms had the strongest influence of any single construct. Consumer acceptance of IBDF is multi-faceted including social, cultural and ethical components, and it is likely that the better availability of information and opportunities for consumers to familiarise themselves with IBDF would help to drive consumer acceptance. In order to allow dog owners to make informed decisions in line with their ethical preferences, further research is needed to establish the overall health and welfare implications of IBDF on the animals involved in production, as well as the companion animals, who are the ultimate consumers.
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(This article belongs to the Special Issue Animal Health: Potential Benefits of Edible Insects)
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The Development of a Risk Assessment Model for Inedible Rendering Plants in Canada: Identifying and Selecting Feed Safety-Related Factors
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Virginie Lachapelle, Geneviève Comeau, Sylvain Quessy, Romina Zanabria, Mohamed Rhouma, Tony van Vonderen, Philip Snelgrove, Djillali Kashi, My-Lien Bosch, John Smillie, Rick Holley, Egan Brockhoff, Marcio Costa, Marie-Lou Gaucher, Younes Chorfi and Manon Racicot
Animals 2024, 14(7), 1020; https://doi.org/10.3390/ani14071020 - 27 Mar 2024
Abstract
The Canadian Food Inspection Agency (CFIA) is developing an establishment-based risk assessment model to categorize rendering plants that produce livestock feed ingredients (ERA-Renderer model) according to animal and human health risks (i.e., feed safety risks) and help in determining the allocation of inspection
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The Canadian Food Inspection Agency (CFIA) is developing an establishment-based risk assessment model to categorize rendering plants that produce livestock feed ingredients (ERA-Renderer model) according to animal and human health risks (i.e., feed safety risks) and help in determining the allocation of inspection resources based on risk. The aim of the present study was to identify and select feed-safety-related factors and assessment criteria for inclusion in the ERA-Renderer model. First, a literature review was performed to identify evidence-based factors that impact the feed safety risk of livestock feed during its rendering processes. Secondly, a refinement process was applied to retain only those that met the inclusion conditions, such as data availability, lack of ambiguity, and measurability. Finally, an expert panel helped in selecting factors and assessment criteria based on their knowledge and experience in the rendering industry. A final list of 32 factors was developed, of which 4 pertained to the inherent risk of a rendering plant, 8 were related to risk mitigation strategies, and 20 referred to the regulatory compliance of a rendering plant. A total of 179 criteria were defined to assess factors based on practices in the Canadian rendering industry. The results of this study will be used in the next step of the model development to estimate the relative risks of the assessment criteria considering their impact on feed safety. Once implemented, the CFIA’s ERA-Renderer model will provide an evidence-based, standardized, and transparent approach to help manage the feed safety risks in Canada’s rendering sector.
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(This article belongs to the Section Public Policy, Politics and Law)
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Trends in Rescue and Rehabilitation of Marsupials Surviving the Australian 2019–2020 Bushfires
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Holly R. Cope, Clare McArthur, Rachael Gray, Thomas M. Newsome, Christopher R. Dickman, Aditi Sriram, Ron Haering and Catherine A. Herbert
Animals 2024, 14(7), 1019; https://doi.org/10.3390/ani14071019 - 27 Mar 2024
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The 2019–2020 Australian bushfire season had a devastating impact on native wildlife. It was estimated that 3 billion native animals were impacted by the fires, yet there are few estimates of the number of animals that were rescued and rehabilitated post-fire. Focusing on
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The 2019–2020 Australian bushfire season had a devastating impact on native wildlife. It was estimated that 3 billion native animals were impacted by the fires, yet there are few estimates of the number of animals that were rescued and rehabilitated post-fire. Focusing on the state of New South Wales (NSW) and Kangaroo Island, South Australia, we used a case study approach to determine the number of marsupials that were reported rescued due to the 2019–2020 bushfires in these areas and analysed species-specific trends in rescue and release success. In NSW, we found 889 reports of fire-affected marsupials in 2019–2020, mostly comprising kangaroos and wallabies (macropods; n = 458), koalas (n = 204), and possums (n = 162), with a smaller number of wombats (n = 43) and other marsupial species. Most reports of fire-affected marsupials occurred 6–8 weeks after fire ignition, and there was no difference in temporal frequency of rescues between marsupial groups. For the three main groups, the probability of survival and subsequent release differed, with macropods having the lowest probability of release after rescue (0.15 ± 0.04) compared to koalas (0.47 ± 0.04) and possums (0.55 ± 0.10). The type of injury was the main predictor of survival during rehabilitation for all three marsupial groups, with those malnourished/moribund or with traumatic injuries less likely to survive rehabilitation. Death or euthanasia occurred on the day of rescue for 77% of macropods, 48% of possums and 15% of koalas. Koalas most often died during rehabilitation rather than on the day of rescue, with 73% either dying or being euthanised between day 1 and 30 post-rescue, representing a potential welfare concern. On Kangaroo Island, koalas were the most frequently rescued marsupial species; most euthanasia cases and deaths occurred in a hospital, whereas other marsupials were mostly euthanised at triage. In both jurisdictions, koalas were over-represented while possums were under-represented relative to baseline population densities and wildlife rescue trends in the years before the 2019–2020 bushfires. These species differences in presentation post-fire warrant further investigation, as do the differences in triage, survival and release outcomes. It is hypothesised that the high intensity and large scale of the 2019–2020 fires impeded marsupial fire evasion tactics, as evidenced by the small number of animals found for rescue, and the differing rates of presentation relative to underlying population densities for the main marsupial groups. Based on our findings, there is a need for detailed record keeping and data sharing, development of consistent and evidence-based triage, treatment and euthanasia guidelines and deployment of trained wildlife emergency rescue teams with advanced search techniques to minimise animal suffering where safe to do so.
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Open AccessArticle
Genetic Diversity of Avian Influenza Viruses Detected in Waterbirds in Northeast Italy Using Two Different Sampling Strategies
by
Giulia Graziosi, Caterina Lupini, Federica Gobbo, Bianca Zecchin, Giulia Quaglia, Sara Pedrazzoli, Gabriele Lizzi, Geremia Dosa, Gabriella Martini, Calogero Terregino and Elena Catelli
Animals 2024, 14(7), 1018; https://doi.org/10.3390/ani14071018 - 27 Mar 2024
Abstract
Avian influenza viruses (AIVs), which circulate endemically in wild aquatic birds, pose a significant threat to poultry and raise concerns for their zoonotic potential. From August 2021 to April 2022, a multi-site cross-sectional study involving active AIV epidemiological monitoring was conducted in wetlands
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Avian influenza viruses (AIVs), which circulate endemically in wild aquatic birds, pose a significant threat to poultry and raise concerns for their zoonotic potential. From August 2021 to April 2022, a multi-site cross-sectional study involving active AIV epidemiological monitoring was conducted in wetlands of the Emilia-Romagna region, northern Italy, adjacent to densely populated poultry areas. A total of 129 cloacal swab samples (CSs) and 407 avian faecal droppings samples (FDs) were collected, with 7 CSs (5.4%) and 4 FDs (1%) testing positive for the AIV matrix gene through rRT-PCR. A COI-barcoding protocol was applied to recognize the species of origin of AIV-positive FDs. Multiple low-pathogenic AIV subtypes were identified, and five of these were isolated, including an H5N3, an H1N1, and three H9N2 in wild ducks. Following whole-genome sequencing, phylogenetic analyses of the hereby obtained strains showed close genetic relationships with AIVs detected in countries along the Black Sea/Mediterranean migratory flyway. Notably, none of the analyzed gene segments were genetically related to HPAI H5N1 viruses of clade 2.3.4.4b isolated from Italian poultry during the concurrent 2021–2022 epidemic. Overall, the detected AIV genetic diversity emphasizes the necessity for ongoing monitoring in wild hosts using diverse sampling strategies and whole-genome sequencing.
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(This article belongs to the Special Issue Interdisciplinary Perspectives on Wildlife Disease Ecology)
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Open AccessArticle
Outcome of the Use of Clinical Reasoning Alone in Dogs with Acute Thoracolumbar Myelopathy and/or Pain
by
Bruno Scalia and Paul Freeman
Animals 2024, 14(7), 1017; https://doi.org/10.3390/ani14071017 - 27 Mar 2024
Abstract
Acute thoracolumbar myelopathy is a common neurological presentation in dogs. Although certain spinal conditions present with characteristic clinical pictures, managing such cases with clinical reasoning alone (i.e., without cross-sectional imaging) has never been explored. The aim of this study was to define the
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Acute thoracolumbar myelopathy is a common neurological presentation in dogs. Although certain spinal conditions present with characteristic clinical pictures, managing such cases with clinical reasoning alone (i.e., without cross-sectional imaging) has never been explored. The aim of this study was to define the outcome of patients with suspected intervertebral disc extrusion (IVDE), ischaemic myelopathy (IM) or acute non-compressive nucleus pulposus extrusion (ANNPE) that were managed with clinical reasoning alone. The database of the Queen’s Veterinary School Hospital (Cambridge) was searched for paraparetic or paraplegic dogs with suspected IVDE or IM/ANNPE that were initially managed medically without undergoing imaging. Clinical presentation and outcome were recorded. If cross-sectional imaging was subsequently performed, information about the final diagnosis was collected and compared with the initially suspected diagnosis. A total of 123 IVDE cases were collected: 81% had a successful outcome with no imaging performed; 16% had IVDE confirmed with imaging and successful outcome with surgery or medical management; and just 3% were found to have an alternative diagnosis or were euthanised without imaging. A total of 16 IM/ANNPE cases were collected: 94% had a successful outcome, and one dog was euthanised. Successful outcomes can be obtained by using clinical reasoning alone in most dogs with suspected IVDE or IM/ANNPE.
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(This article belongs to the Section Veterinary Clinical Studies)
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Open AccessArticle
Rumen-Protected Choline Improves Metabolism and Lactation Performance in Dairy Cows
by
Fábio Soares de Lima, Manoel Francisco Sá Filho, Leandro Ferreira Greco and José Eduardo Portela Santos
Animals 2024, 14(7), 1016; https://doi.org/10.3390/ani14071016 - 27 Mar 2024
Abstract
Choline is required for the synthesis of phosphatidylcholine, an important constituent of lipoproteins. Early lactation cows presumably synthesize insufficient phosphatidylcholine, and choline supplementation in a rumen-protected form might benefit metabolism and lactation. The objectives of this study were to determine the effects of
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Choline is required for the synthesis of phosphatidylcholine, an important constituent of lipoproteins. Early lactation cows presumably synthesize insufficient phosphatidylcholine, and choline supplementation in a rumen-protected form might benefit metabolism and lactation. The objectives of this study were to determine the effects of feeding rumen-protected choline (RPC) on lactation and metabolism in dairy cows. In experiment 1, 369 nulliparous and parous Holstein cows housed in four pens per treatment were fed 12.9 g/day of choline as RPC from 25 days prepartum until 80 days postpartum. In experiment 2, 578 nulliparous cows housed in five pens/treatment were fed 12.9 g/day of choline as RPC in the last 22 days of gestation only. In both experiments, blood was sampled and analyzed for concentrations of nonesterified fatty acids (NEFAs) and glucose at 1, 14, and 21 days postpartum and of choline at 1 and 14 days postpartum. Blood from all cows was sampled and analyzed for concentrations of β-OH butyrate (BHB) at 1 and 14 days postpartum. Cows with BHB > 1.2 mmol/L were classified as having hyperketonemia. Hepatic tissue was collected from 46 cows from the eight pens in experiment 1 at 9 days postpartum and analyzed for concentrations of glycogen and triacylglycerol. Milk yield and components were measured for 80 days postpartum in experiment 1, whereas only milk yield was measured in experiment 2. The pen was the experimental unit of analysis. Supplementing RPC tended to increase dry matter intake (DMI) prepartum in experiments 1 and 2 and postpartum in experiment 1. Feeding cows with RPC increased yields of 3.5% fat-corrected milk (42.8 vs. 44.8 kg/day), energy-corrected milk (38.5 vs. 40.3 kg/day), milk fat (1.52 vs. 1.61 kg/day), and true protein (1.16 vs. 1.21 kg/day) in experiment 1. Milk yield tended to be greater with RPC (26.4 vs. 27.4 kg/day) in experiment 2. Supplementing RPC increased plasma choline concentrations on day 14 postpartum in experiment 1 (3.32 ± 0.27 vs. 4.34 ± 0.28 µM) and on day 1 in experiment 2 (3.35 ± 0.16 and 13.73 ± 0.15 µM). Treatment did not affect the concentrations of glucose, NEFAs, or BHB in plasma, but the incidence of hyperketonemia was less in multiparous cows fed RPC than those fed the control in experiment 1. Feeding cows with RPC reduced hepatic triacylglycerol content and tended to reduce the ratio of triacylglycerol to glycogen and the risk of hepatic lipidosis in cows in experiment 1. The concentrations of hepatic triacylglycerol on day 9 postpartum were inversely related to those of choline in plasma on day 1 postpartum. Feeding cows with RPC improved lactation and metabolism, but more benefits were noted when it was fed before and after calving.
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(This article belongs to the Special Issue Research Advances in Dairy Cow Nutrition)
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Open AccessReview
Coccidiosis in Egg-Laying Hens and Potential Nutritional Strategies to Modulate Performance, Gut Health, and Immune Response
by
Milan Kumar Sharma and Woo Kyun Kim
Animals 2024, 14(7), 1015; https://doi.org/10.3390/ani14071015 - 27 Mar 2024
Abstract
Avian coccidiosis, despite advancements in management, nutrition, genetics, and immunology, still remains the most impactful disease, imposing substantial economic losses to the poultry industry. Coccidiosis may strike any avian species, and it may be mild to severe, depending on the pathogenicity of Eimeria
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Avian coccidiosis, despite advancements in management, nutrition, genetics, and immunology, still remains the most impactful disease, imposing substantial economic losses to the poultry industry. Coccidiosis may strike any avian species, and it may be mild to severe, depending on the pathogenicity of Eimeria spp. and the number of oocysts ingested by the bird. Unlike broilers, low emphasis has been given to laying hens. Coccidiosis in laying hens damages the gastrointestinal tract and causes physiological changes, including oxidative stress, immunosuppression, and inflammatory changes, leading to reduced feed intake and a drastic drop in egg production. Several countries around the world have large numbers of hens raised in cage-free/free-range facilities, and coccidiosis has already become one of the many problems that producers have to face in the future. However, limited research has been conducted on egg-laying hens, and our understanding of the physiological changes following coccidiosis in hens relies heavily on studies conducted on broilers. The aim of this review is to summarize the effect of coccidiosis in laying hens to an extent and correlate it with the physiological changes that occur in broilers following coccidiosis. Additionally, this review tries to explore the nutritional strategies successfully used in broilers to mitigate the negative effects of coccidiosis in improving the gut health and performance of broilers and if they can be used in laying hens.
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(This article belongs to the Special Issue Coccidiosis in Poultry: Current Thinking on Eimeria spp., Host–Parasite Relationships, and Potential for Advancements in Control)
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Open AccessCommunication
How Do Escape Distance Behavior of Broiler Chickens Change in Response to a Mobile Robot Moving at Two Different Speeds?
by
Glauber da Rocha Balthazar, Robson Mateus Freitas Silveira and Iran José Oliveira da Silva
Animals 2024, 14(7), 1014; https://doi.org/10.3390/ani14071014 - 27 Mar 2024
Abstract
In poultry farming, robots are considered by birds as intruder elements to their environment, because animals escape due to their movement. Their escape is measured using the escape distance (ED) technique. This study analyzes the behavior of animals in relation to their ED
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In poultry farming, robots are considered by birds as intruder elements to their environment, because animals escape due to their movement. Their escape is measured using the escape distance (ED) technique. This study analyzes the behavior of animals in relation to their ED through the use of a robot with two speeds: 12 rpm and 26 rpm. The objective is to understand whether the speeds cause variations in ED and their implications for animal stress. A broiler breeding cycle was analyzed (six weeks) through the introduction of the robot weekly. ED analyses were carried out on static images generated from footage of the robot running. The results indicate higher escape distance rates (p < 0.05) peaking midway through the production cycle, notably in the third week. Conversely, the final weeks saw the lowest ED, with the most significant reduction occurring in the last week. This pattern indicates a gradual escalation of ED up to the fourth week, followed by a subsequent decline. Despite RPM12 having shown low ED results, it did not show enough ED to move the animals away from their path of travel, causing bumps and collisions. RPM26 showed higher ED in all breeding phases, but showed ED with no bumps and collisions.
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(This article belongs to the Section Poultry)
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Clinical and Clinico-Pathological Observations of the Erythrocyte Sedimentation Rate in Dogs Affected by Leishmaniosis and Other Inflammatory Diseases
by
George Lubas, Saverio Paltrinieri, Roberto Amerigo Papini, Ilaria Lensi, Silvia Lucia Benali, Oscar Cortadellas, Nunzio D’Anna, Alessandra Fondati, Xavier Roura and Eric Zini
Animals 2024, 14(7), 1013; https://doi.org/10.3390/ani14071013 - 27 Mar 2024
Abstract
The erythrocyte sedimentation rate (ESR) has been used in canine medicine in several disorders, above all, to evaluate levels of inflammation. This study evaluated the ESR in canine leishmaniosis (CanL) and other inflammatory conditions. Three groups of dogs were examined: CanL affected dogs
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The erythrocyte sedimentation rate (ESR) has been used in canine medicine in several disorders, above all, to evaluate levels of inflammation. This study evaluated the ESR in canine leishmaniosis (CanL) and other inflammatory conditions. Three groups of dogs were examined: CanL affected dogs without clinical signs (INFECTED group, #25) or with clinical signs (SICK group, #43) and dogs affected by acute or acute-on-chronic conditions (OTHER DISEASE group, #65). The ESR was compared with acute phase proteins or reactants either positive or negative (leukogram, fibrinogen, iron, unsaturated iron binding capacity, ferritin, haptoglobin, and albumin) and immunological markers (gamma-globulins, IgG, and IgM). The ESR was higher in the SICK group than in the INFECTED group (median 39 vs. 11 mm/h; p < 0.0001), as well as in the OTHER DISEASE than in the INFECTED groups (median 41 vs. 11 mm/h; p < 0.0001). The ESR appeared outside the reference range for all dogs in the SICK and OTHER DISEASE groups and almost with similar values (mm/h; median 39, 95% CI 31–51 vs. 41, 95% CI 12–87; p > 0.05). The extent of changes in ESR can help to establish the severity of CanL and other inflammatory disorders. As a point-of-care test, the ESR can be used to screen dogs for unhealthy conditions, and its values correlate with the severity of any disease, including CanL.
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(This article belongs to the Section Veterinary Clinical Studies)
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