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BEHAV3D is a 3D live imaging platform for analyzing engineered T cell behavior and tumor response. This provides insights into the mode of action of cellular immunotherapy, capturing heterogeneity within and between tumors during treatment response.
We provide a twisting fabrication process for fiber electrodes that can be assembled into electronic threads and then integrated in electronic textile-based wearables.
The authors describe how to prepare antimicrobial phage-based microgels, using polystyrene film templates, and detail their use to kill antibiotic-resistant bacteria in food contamination tests as an example application.
This protocol provides detailed guidelines for using rMATS-turbo—the latest implementation of the popular software for the discovery and quantification of alternative splicing events from RNA sequencing data—exemplified in two representative scenarios.
The Protocol Exchange is an open repository of community-contributed protocols. See the latest, including highly scalable multiplex serology by mass spectrometry analysis of beads, microCT scanning and analysis in the zebrafish skeleton, and DPC-seq: genome-wide mapping of DNA-protein crosslinks (DPC) induced by endogenous aldehydes.
Our authors are invited to write blog posts that describe how they conceived and developed their protocols, prior to publication at Nature Protocols. These stories are published on a community website for researchers who are interested in techniques and methods.
This Collection highlights articles on how to improve methodological clarity in science publications from a spectrum of different journals and subject areas.
Isotopically labeled amino acids are useful in pharmacology and for medical imaging. In this protocol, C1-labeled α-amino acids are prepared via late-stage carboxylate exchange of unprotected α-amino acids with [*C]CO2 where *C is 13C, 11C or 14C.
We present a protocol for achieving efficient generation of hPSC-CM aggregates in suspension culture, emphasizing process simplicity, robustness and GMP compliance. The strategy promotes clinical translation and other applications that require large numbers of CMs.
This protocol can be used to generate three-dimensional vascularized bone marrow organoids from human induced pluripotent stem cells. The organoids contain key stromal and hematopoietic cell types and can be engrafted with normal and malignant cells from adult donors to model niche interactions.
Cell engineering using polymeric material is an area that remains largely unexplored. This protocol describes two light-driven approaches for synthesizing bioactive polymers within intricate intracellular settings.
This protocol is for using PanSyn, the first software package for the identification of micro- and macrosynteny and their functional integration for comprehensive characterization of genome architecture and regulatory evolution.
Genetic interactions have been found to influence phenotypes in a variety of systems, yet their specific contribution to complex diseases remains unclear. This protocol describes Bridging Gene sets with Epistasis (BridGE), a computational approach for discovering interactions between biological pathways from genome-wide association studies data.
The protocol presents for an optimized culture system for deriving porcine expanded potential stem cells from preimplantation embryos and reprogrammed somatic cells, and for validation and characterization.
This protocol presents a method for single-cell RNA sequencing of tissue-resident murine eosinophils, with a complementary method for CRISPR screening of bone marrow-derived eosinophils.
BEHAV3D is a 3D live imaging platform for analyzing engineered T cell behavior and tumor response. This provides insights into the mode of action of cellular immunotherapy, capturing heterogeneity within and between tumors during treatment response.