ALBERT

Description: Optical techniques of photobiostimulation, which use transducer as lasers and LEDs, have been employed in the treatment of several diseases. The laser systems usually irradiate in a reduced area of the target biological tissue, presenting high cost of acquisition. Devices with a LED of “Surface Mount Device” (SMD) type have high angle of light emission, implying in intense decrease of the density of optical potency. Furthermore, the use of this device requires the utilization of a cooling system, which provokes an increase in the cost of this product. In the present work, it is suggested the application of a novel opto-mechanical device constituted by usual LEDs with package dimension of 5 mm of diameter, which presents significant efficient and very low cost. The arrangement of the geometric configuration of these LEDs is elaborated in such way that all units emit photons in a unique section of an specific area. An opto-mechanical system was developed, which includes seven LEDs, being that six of these LEDs are disposed in a specific angle around a unique central LED. It is important to notice that all the opto-mechanical system with seven LEDs emit light in a unique area. Besides, a methodology was employed to allow the verification of the distribution of light intensity upon this respective area. This arrangement allows that this novel device propitiate measurements of the degree of homogeneity of the light intensity in specific areas, which are accessed by the seven light emitting units. The potency employing LEDs in the red (visible) and near infrared regions were also measured, being that the result demonstrated the capability of clinical application in optical therapy of photobiostimulation, which was also used in a pilot test of clinical application. Content Type Journal Article Pages 9-18 DOI 10.3233/SPE-2012-0567 Authors Celso J. Barbosa, Laboratório de Instrumentação Biomédica, Centro de Engenharia Biomédica, Universidade Camilo Castelo Branco, Parque Tecnológico de São José dos Campos, São José dos Campos, SP, Brazil Crisaldo Teles, Laboratório de Instrumentação Biomédica, Centro de Engenharia Biomédica, Universidade Camilo Castelo Branco, Parque Tecnológico de São José dos Campos, São José dos Campos, SP, Brazil Leonardo M. Moreira, Universidade Federal de São João Del Rei, São João Del Rei, MG, Brazil Álvaro J. Damião, Laboratório de Medição de Superfícies Ópticas-LMSO-IEAv-CTA, São José dos Campos, São Paulo, SP, Brazil Carlos J. de Lima, Laboratório de Instrumentação Biomédica, Centro de Engenharia Biomédica, Universidade Camilo Castelo Branco, Parque Tecnológico de São José dos Campos, São José dos Campos, SP, Brazil Journal Spectroscopy: An International Journal Online ISSN 1875-922X Print ISSN 0712-4813 Journal Volume Volume 27 Journal Issue Volume 27, Number 1 / 2012

Description: The interaction between bovine serum albumin (BSA) and Prodigiosin (PG) was investigated by UV-vis absorption, fluorescence, synchronous fluorescence, FT-IR and circular dichroism (CD) techniques. The data of UV-vis absorption and fluorescence spectra displayed that there existed interaction between PG and aromatic amino acid residues of BSA. The synchronous fluorescence and CD spectrum experiment both showed that the secondary structure of BSA changed with addition of PG. All these results revealed that the conformation and microenvironment of BSA were changed. Content Type Journal Article Pages 19-26 DOI 10.3233/SPE-2012-0561 Authors Shu-Chao Liu, College of Life Science, Nanjing Normal University, Nanjing, China Jing Tang, Center for Analysis and Testing, Nanjing Normal University, Nanjing, China Xi-Hai Zhang, College of Life Science, Nanjing Normal University, Nanjing, China Yuan-Yuan Gao, College of Life Science, Nanjing Normal University, Nanjing, China Fei Ma, Center for Analysis and Testing, Nanjing Normal University, Nanjing, China Qi-yin Yang, College of Life Science, Nanjing Normal University, Nanjing, China Journal Spectroscopy: An International Journal Online ISSN 1875-922X Print ISSN 0712-4813 Journal Volume Volume 27 Journal Issue Volume 27, Number 1 / 2012

Description: The evaluation of the electronic charge distribution in metal complexes enables more precise interpretation of mechanism by which particular metal ions affect biochemical properties of ligands [J. Inorg. Biochem. 99 (2005), 1407–1423, J. Mol. Struct. 919 (2009), 284–289]. In this paper we investigated the influence of alkali metal cations (lithium, sodium, potassium, rubidium and cesium) on the electronic structure of p-coumaric acid (p-CA). It allowed to observe the systematic changes in the spectra of investigated complexes depending on the position of the element in the periodic table. p-Coumaric acid is a derivative of cinnamic acid that occurs in several plant species. Li, Na, K, Rb and Cs p-coumarates were synthesized and the experimental and theoretical FT-IR, FT-Raman, 1 H and 13 C NMR spectra of p-coumaric acid and its salts were registered and analyzed. The structures, atomic charges, infrared and NMR spectra of p-coumaric acid and Li, Na, K salts were calculated by B3LYP/6-311++G ** method. Content Type Journal Article Pages 35-48 DOI 10.3233/SPE-2012-0568 Authors Renata Świsłocka, Division of Chemistry, Bialystok University of Technology, Bialystok, Poland Małgorzata Kowczyk-Sadowy, Division of Chemistry, Bialystok University of Technology, Bialystok, Poland Monika Kalinowska, Division of Chemistry, Bialystok University of Technology, Bialystok, Poland Włodzimierz Lewandowski, Division of Chemistry, Bialystok University of Technology, Bialystok, Poland Journal Spectroscopy: An International Journal Online ISSN 1875-922X Print ISSN 0712-4813 Journal Volume Volume 27 Journal Issue Volume 27, Number 1 / 2012

Description: Salt formation is a good method of increasing solubility, dissolution rate and consequently the bioavailability of poor soluble acidic or basic drugs [Polymorphism in Pharmaceutical Solids, Drugs and the Pharmaceutical Sciences, Vol. 192, 2nd edn, Informa Healthcare, New York, 2009]. The aim of this study was to obtain and to investigate the structural properties of the compound obtained by solvent drop grinding (SDG) method [Chem. Commun. (Camb.) 20 (2002), 2372–2373] at room temperature starting from the 1:1 molar ratios of ambazone (AMB) and niflumic acid (NIA). The thermal behavior of the obtained compound (AMB · NIA) was investigated by differential scanning calorimetry (DSC) and thermogravimetry (TG), the thermal diffusivity and effusivity parameters were determined by photothermal radiometry (PTR). The structural characterization was performed with X-ray powder diffraction (XRPD) and infrared spectroscopy (FTIR). XRPD data and FTIR spectra demonstrated a new structure for AMB · NIA compound as compared to those of the starting materials. Content Type Journal Article Pages 49-58 DOI 10.3233/SPE-2012-0570 Authors Irina Kacso, National Institute for Research and Development of Isotopic and Molecular Technologies, Cluj-Napoca, Romania Lucia Rus, Department of Drug Analysis, Faculty of Pharmacy, University of Medicine and Pharmacy, Cluj-Napoca, Romania Mircea Pop, National Institute for Research and Development of Isotopic and Molecular Technologies, Cluj-Napoca, Romania Gheorghe Borodi, National Institute for Research and Development of Isotopic and Molecular Technologies, Cluj-Napoca, Romania Ioan Bratu, National Institute for Research and Development of Isotopic and Molecular Technologies, Cluj-Napoca, Romania Journal Spectroscopy: An International Journal Online ISSN 1875-922X Print ISSN 0712-4813 Journal Volume Volume 27 Journal Issue Volume 27, Number 1 / 2012

Description: Our work aims to explore Raman spectroscopy (RS) to study the effects of total protein of nasopharyngeal carcinoma (NPC) cell line CNE2 treated by different doses of X-ray irradiation. The total protein is extracted from the cell line after irradiation with no incubation (0 h) and with incubation for 72 h, respectively. Both paired-samples t test and support vector machine (SVM) are employed for statistical analysis of Raman spectrum. The results show that several Raman bands assigned to total protein can be detected; and the X-ray irradiation have various effects on different components in total protein, especially for the dose higher than 6 Gy, and also associated with the incubation time after irradiation. The differences of content between 0 h groups and 72 h groups appear at bands of C–S stretching, C–H stretching, O=C–O − stretching and L-phenylalanine. It indicated no significant diversification on structures of protein, while the content may be varied during irradiation. Our spectroscopic results reveal that RS analysis of total protein of cell line irradiated by X-ray, in conjunction with mathematical statistical model, can be a potential method to explore the Raman characterization target of cell radiosensitivity, and then for making known the mechanism of radiosensitivity of tumor further. Content Type Journal Article Pages 1-7 DOI 10.3233/SPE-2012-0569 Authors Lin Ou, Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, China Yang Chen, Zhicheng College, Fuzhou University, Fuzhou, China Yangwen Huang, Key Laboratory of Instrumentation Science & Dynamic Measurement, Ministry of Education, North University of China, Taiyuan, China Ying Su, Fujian Provincial Tumor Hospital, Fuzhou, China Duo Lin, Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, China Changyan Zou, Fujian Provincial Tumor Hospital, Fuzhou, China Jinping Lei, Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, China Journal Spectroscopy: An International Journal Online ISSN 1875-922X Print ISSN 0712-4813 Journal Volume Volume 27 Journal Issue Volume 27, Number 1 / 2012

Description: In our previous study thermodynamic denaturation of bovine β-lactoglobulin variant A (BLG-A), has been investigated in the presence of cetylpyridinium chloride (CPC) as a cationic surfactant. Here, the retinol binding property of BLG was determined at 298 K and pH 8.0 by spectrofluorimeter titration method, in the presence of CPC to elucidate the still unknown structure–function relationship in this protein. Comparison of the results allowed determining the binding of retinol by BLG in the presence of CPC. The two-way chemometrics method was used, to estimate the equilibrium concentration of components by analysis of fluorescence emission spectrum, in order to obtain its equilibrium concentration. The results indicate that the retinol binding properties of BLG do not show significant changes in the presence of this surfactant. Content Type Journal Article Pages 27-34 DOI 10.3233/SPE-2012-0565 Authors Mehdi Sahihi, Department of Chemistry, Isfahan University of Technology, Isfahan, Iran Yousef Ghayeb, Department of Chemistry, Isfahan University of Technology, Isfahan, Iran Abdol-Khalegh Bordbar, Department of Chemistry, University of Isfahan, Isfahan, Iran Journal Spectroscopy: An International Journal Online ISSN 1875-922X Print ISSN 0712-4813 Journal Volume Volume 27 Journal Issue Volume 27, Number 1 / 2012

Description: Adipogenesis plays an important role in energy homeostasis by storing excess energy as lipid droplets. However, these reservoirs are implicated in a host of major human health problems, such as obesity. Elucidation of the mechanisms underlying adipogenesis is thus crucial to overcome these problems. The preadipocyte cell lines represent an optimal model to examine adipogenesis. Cells differentiate into adipocytes with various speeds of conversion and fat accumulation. Here, we have presented a novel method for detecting adipogenic differentiation at the single-cell level using atomic force microscopic analysis. Data obtained with this method revealed a good correlation between membrane stiffness and the degree of adipogenic differentiation. Although we could not determine the underlying cause for membrane stiffness reduction during adipogenic differentiation, the technique clearly offers advantages over the existing detection systems, such as lipid drop staining and extraction. Furthermore, the degree of adipogenic differentiation at the single-cell level can be detected with this method. Content Type Journal Article Pages 329-335 DOI 10.3233/SPE-2012-0566 Authors Young-Nam Kwon, Samsung Advanced Institute of Technology, Kyung-gi, Korea Won Kon Kim, Analytical Science Group, Medical Proteomics Research Center, KRIBB, Daejeon, Korea Sang-Hak Lee, Department of Chemistry, Seoul National University, Seoul, Korea Keewon Kim, Samsung Advanced Institute of Technology, Kyung-gi, Korea Eun Young Kim, Analytical Science Group, Medical Proteomics Research Center, KRIBB, Daejeon, Korea Tai Hwan Ha, Immunotherapy Research Center, KRIBB, Daejeon, Korea HyoukSoo Han, Samsung Advanced Institute of Technology, Kyung-gi, Korea Kwang-Hee Bae, Analytical Science Group, Medical Proteomics Research Center, KRIBB, Daejeon, Korea Journal Spectroscopy: Biomedical Applications Online ISSN 1875-922X Print ISSN 0712-4813 Journal Volume Volume 26 Journal Issue Volume 26, Number 6 / 2011

Description: The interaction between anionic form of copper (II) N,N′,N″,N‴-tetrasulfonated phthalocyanine Cu (tspc) and to calf thymus deoxyribonucleic acid (ct-DNA) is investigated by measuring UV-vis absorption and fluorescence spectroscopy in phosphate buffer. The binding constant and stoichiometry were determined by analysis of optical absorption spectra of phthalocyanine at various ct-DNA concentrations using SQUAD software. The static mode of fluorescence quenching of phthalocyanine by calf thymus deoxyribonucleic acid indicates the formation of a ground-state complex. The formation of ground-state complex is a spontaneous molecular interaction procedure in which outside groove binding through the formation of an axial bond between the base pairs of nucleotide and Cu in the central core of phthalocyanine. Content Type Journal Article Pages 357-365 DOI 10.3233/SPE-2012-0564 Authors Hamid Dezhampanah, Laboratory of Physical Chemistry, Department of Chemistry, Faculty of Science, University of Guilan, Rasht, Iran Termeh Darvishzad, Laboratory of Physical Chemistry, Department of Chemistry, Faculty of Science, University of Guilan, Rasht, Iran Mehrnaz Aghazadeh, Laboratory of Physical Chemistry, Department of Chemistry, Faculty of Science, University of Guilan, Rasht, Iran Journal Spectroscopy: Biomedical Applications Online ISSN 1875-922X Print ISSN 0712-4813 Journal Volume Volume 26 Journal Issue Volume 26, Number 6 / 2011

Description: Royal jelly is the mixture of acids like 2-Decenoic acid (DA), 10-Hydroxy 2-Decenoic acid and many others. 10-HDA possesses strong inhibition to the growth of cancer cells, namely transferable AKR leukaemia, TA3 breast cancer, etc. The equilibrium geometry of 2-Decenoic acid and its derivative 10-Hydroxy 2-Decenoic acid in the ground state have been determined and analyzed by Density functional theory (DFT) employing 6-311 G (d, p) as the basis set. The harmonic frequencies of 2-DA and 10-HDA have also been calculated to understand its complete vibrational dynamics. The study of simulated spectra provides important information about the ability of the computational method to describe the vibrational modes. Content Type Journal Article Pages 367-385 DOI 10.3233/SPE-2012-0562 Authors Apoorva Dwivedi, Department of Physics, Lucknow University, Lucknow, India Anoop Kumar Pandey, Department of Physics, Lucknow University, Lucknow, India Neeraj Misra, Department of Physics, Lucknow University, Lucknow, India Journal Spectroscopy: Biomedical Applications Online ISSN 1875-922X Print ISSN 0712-4813 Journal Volume Volume 26 Journal Issue Volume 26, Number 6 / 2011

Description: An ultrasensitive method for determining picomolar midecamycin (MID) by flow injection (FI) chemiluminescence (CL) was first described based on the inhibitory effect of MID on luminol–BSA reaction. It was found that the CL intensity decrements were linear with the logarithm of MID concentrations in the range of 1.0–5000 pmol·l −1 with a detection limit as low as 0.3 pmol·l −1 (3σ). The relative standard deviation of seven repetitive measurements for 10 pmol·l −1 MID was 3.0%. At a flow rate of 2.0 ml·min −1 , the whole analysis procedure including sampling and washing could be finished in 30 s, offering the sample efficiency of 120 h −1 . This proposed method was successfully applied to determine MID in human serum samples with the recoveries from 96.0 to 110.0%. The CL mechanism of luminol–BSA–MID reaction was also given. Content Type Journal Article Pages 349-355 DOI 10.3233/SPE-2012-0563 Authors Hairu Lv, Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Material Science, Northwest University, Xi'an, China Xijuan Tan, Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Material Science, Northwest University, Xi'an, China Yun Zhang, Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Material Science, Northwest University, Xi'an, China Zhenghua Song, Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Material Science, Northwest University, Xi'an, China Journal Spectroscopy: Biomedical Applications Online ISSN 1875-922X Print ISSN 0712-4813 Journal Volume Volume 26 Journal Issue Volume 26, Number 6 / 2011