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Fragmentation of condensed chromatin in nuclei of a plant sperm, pteridium aquilinum (L.) Kuhn (1980)

Wakeford, R. J. ; Bell, P. R.

New York, NY : Wiley-Blackwell

Gamete Research 3 (1980), S. 291-298

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ISSN: 0148-7280

Keywords: condensed chromatin ; sperm ; Pteridium ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology

Notes: Nuclei of pteridium sperm have been dispersed by turbulence in natural or slightly alkaline buffer after stripping off the cytoplasm with nonionic detergent. The nuclei tended to break up into fragments arranged in a linear order. These fragments fluoresced brightly with acridine orange as did intact nuclei. Grounds are given for identifying the smaller fragments with chromosomes. It is proposed that the sperm nucleus of British Pteridium, possibly an autotetrapolid, consists of a sequence of paired homologues.

Additional Material: 8 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/mrd.1120030311

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