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  • Transformation  (4)
  • 1
    Electronic Resource
    Electronic Resource
    The promoter of aBrassica napus polygalacturonase gene directs pollen expression ofβ-glucuronidase in transgenicBrassica plants (1997)
    Springer
    Plant cell reports 16 (1997), S. 373-378 
    Details
    ISSN: 1432-203X
    Keywords: Pollen ; Polygalacturonase ; Promoter ; Brassica ; Transformation ; GUS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A 647-bp 5′-flanking fragment obtained from genomic clone Sta 44G(2) belonging to a family of polygalacturonase genes expressed inBrassica napus pollen was fused to theβ-glucuronidase (GUS) marker gene. This fusion construct was introduced intoB. napus plants viaAgrobacterium tumefaciens transformation. Analysis of the transgenicB. napus plants revealed that this promoter fragment is sufficient to direct GUS expression specifically in the anther and that GUS activity increases in pollen during maturation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01146776
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  • 2
    Electronic Resource
    Electronic Resource
    The promoter of a Brassica napus polygalacturonase gene directs pollen expression of β-glucuronidase in transgenic Brassica plants (1997)
    Springer
    Plant cell reports 16 (1997), S. 373-378 
    Details
    ISSN: 1432-203X
    Keywords: Key words Pollen ; Polygalacturonase ; Promoter ; Brassica ; Transformation ; GUS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A 647-bp 5′-flanking fragment obtained from genomic clone Sta 44G(2) belonging to a family of polygalacturonase genes expressed in Brassica napus pollen was fused to the β-glucuronidase (GUS) marker gene. This fusion construct was introduced into B. napus plants via Agrobacterium tumefaciens transformation. Analysis of the transgenic B. napus plants revealed that this promoter fragment is sufficient to direct GUS expression specifically in the anther and that GUS activity increases in pollen during maturation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01146776
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Development of an efficient Agrobacterium-mediated transformation system for Brassica carinata (1998)
    Springer
    Plant cell reports 17 (1998), S. 183-188 
    Details
    ISSN: 1432-203X
    Keywords: Key words Brassica ; Mustard ; Transgenic ; Transformation ; Regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shoot organogenesis and plant regeneration were readily achieved from cotyledonary petioles and hypocotyls of Brassica carinata. These explants were used for Agrobacterium-mediated transformation. A construct containing the selectable marker genes, neomycin phosphotransferase II, phosphinothricin acetyl transferase and the reporter gene β-glucuronidase, under the control of a tandem 35S promoter, was used for transformation. Although transformation was achieved with both cotyledonary petioles and hypocotyls, cotyledonary petioles responded best, with 30–50% of the explants producing GUS-positive shoots after selection on 25 mg/l kanamycin. Direct selection on L-phosphinothricin also produced resistant shoots but at a lower frequency (1–2%).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050375
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  • 4
    Electronic Resource
    Electronic Resource
    Fertile somatic hybrids between transgenicNicotiana tabacum and transgenicN. debneyi selected by dual-antibiotic resistance (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 450-456 
    Details
    ISSN: 1432-2242
    Keywords: Nicotiana tabacum ; N. Debneyi ; Somatic hybridization ; Transformation ; Organelle inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A simple, yet effective selection system was used to produce fertile somatic hybrids betweenNicotiana tabacum andN. debneyi. This approach utilized transgenic antibiotic-resistantN. tabacum andN. Debneyi as donor plants for mesophyll protoplast fusions. Thirteen somatic hybrid plants were regenerated from calli capable of growth on medium containing both antibiotics. The majority of the hybrids displayed a range of leaf and floral morphologies and growth habits that were intermediate to those of the parental species, and had chromosome numbers varying from amphidiploid (2n = 96) to hypoaneuploid (2n = 60). Isoenzyme and RFLP analysis demonstrated the presence and expression of nuclear genes from both parents in all of the hybrids. Most plants are fully fertile. Thus, these plants differ from the malesterile tobacco ‘cybrids’ and alloplasmic lines produced by transferring theN. debneyi cytoplasm to tobacco. A nonrandom pattern of cytoplasmic segregation in the fusion products occurred with a bias towards the presence ofN. debneyi cp and mtDNA. Evidence for the presence of rearranged or recombinant cp and mtDNA in some of the hybrids was obtained. The somatic hybrids were successfully backcrossed to theN. tabacum parent and are now being tested for immunity to black root rot, a trait specific toN. debneyi, but not existent in theN. tabacum parental line.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00588598
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