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  • 1
    Publication Date: 2021-05-19
    Description: The WSD is the important viral shrimp disease in past decade. The detection of virus in each country was investigated by polymerase chain reaction for sensitivity and accurate. In this research study we collected 23 samples from shrimp suspected to WSD. The DNA from samples collected and extracted and design two kind of primer from VP24 identified in gene bank by DNAsis software. A primer also designs for House keeping gene for positive and negative samples in all examined. The results showed the gene colon for wssv is the similar with others and 97% is consistency. The product of PCR was colon in plasmid and confirmed and this plasmid used for internal control.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: White spot syndrome virus ; DNA ; Polymerase chain reaction ; WSD ; Viral ; Shrimp ; Samples ; Penaeus indicus ; Polymerase ; WSSV ; Virus
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 39pp.
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  • 2
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25123 | 18721 | 2018-08-26 13:40:27 | 25123 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The WSD is the important viral shrimp disease in past decade. The detection of virus in each country was investigated by polymerase chain reaction for sensitivity and accurate. In this research study we collected 23 samples from shrimp suspected to WSD. The DNA from samples collected and extracted and design two kind of primer from VP24 identified in gene bank by DNAsis software. A primer also designs for Housekeeping gene for positive and negative samples in all examined. The results showed the gene colon for wssv is the similar with others and 97% is consistency. The product of PCR was colon in plasmid and confirmed and this plasmid used for internal control.
    Keywords: Health ; Iran ; White spot syndrome virus ; DNA ; Polymerase chain reaction ; WSD ; Viral ; Shrimp ; Samples ; Penaeus indicus ; Polymerase ; WSSV ; Virus
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 39
    Location Call Number Expected Availability
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