ISSN:
1432-2242
Keywords:
Cytoplasmic male sterility
;
Mitochondrial DNA
;
Cytoplasm-type assay
;
RNA
;
Plantago lanceolata L
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary To establish the feasibility of a cytoplasmtype assay based on molecular hybridizations, mitochondrial DNAs from the two male-sterile cytoplasms of Plantago lanceolata (P and R) were compared by restriction endonuclease digestion. We cloned a 1.1 kbp Eco RI-HindIII fragment from P-mtDNA (pPl-311), which on hybridization to Southern blots of Bam HI digested mtDNA and total DNA from plants with P-cytoplasm, hybridized to a specific 1.1 kbp Bam HI fragment. Hybridizations of pPl-311 to Northern blots of total RNA resulted in the detection of 1,250 and 1,100 nucleotides transcripts specific to P- and R-cytoplasm, respectively, and of a common transcript of 930 nt. Evidence was obtained for additional cytoplasmic variation within P. lanceolata populations.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00249143
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