ISSN:
1615-6102
Keywords:
Catalase
;
Cytochemistry
;
Ozone
;
Peroxisomes
;
Picea abies
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Catalase (EC 1.11.1.6) activity (both total and specific activity) of particulate fractions of needles of Norway spruce [Picea abies (L.) Karst.] was elevated approximately 2-fold following exposure of trees to 60–70 μg/m3 of ozone during the growing season compared to trees receiving charcoal filtered air (about 15 μg/m3 ozone). Measurements were from homogenates fractionated into particulate and soluble (supernatent) activities. In contrast, the catalase activity of the supernatant was unchanged in response to ozone treatment. Catalase activity declined as the needles aged comparing current, 1-, and 2-year needles but the ozone-induced increment remained constant. Electron microscope cytochemistry using peroxidatic coupling with 3,3′-diaminobenzidine carried out in parallel, revealed catalase-containing peroxisomes both in situ and in the particulate fractions analyzed for catalase activity. The tissue volume occupied by peroxisomes in response to needle age and ozone appeared to vary approximately in proportion to the measured catalase activity. Overall cytochemical reactivity for catalase declined with needle age, but, for all years, was greater in needles of trees receiving air supplemented with ozone compared to those of trees receiving charcoal filtered air.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01322615
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