ISSN:
1573-5028
Keywords:
cyclophilin
;
Oryza sativa
;
peptidyl-prolylcis-trans isomerase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract cDNA clones were isolated and sequenced that encode two related but distinct rice cyclophilins,Cyp1 andCyp2. The predicted amino acid sequences of each are 72% identical to human T-cell cyclophilin. Genomic DNA gel blot analysis suggests cyclophilins in rice are encoded by a small, 6–10-member gene family. BothCyp1 andCyp2 have seven extra amino acid residues in the N-terminal portion of the proteins that are not found in human or other non-plant cyclophilins, suggesting that this is a characteristic of plant cyclophilins.Cyp2 was expressed as 1000 nt transcripts in leaf and root tissues.Cyp1 was expressed as 800 and 900 nt transcripts. Whereas the 900 nt transcript was present in both root and leaf mRNA, the 800 nt transcript was only detectable in root mRNA. A genomic clone ofCyp2 was isolated, sequenced and shown to lack introns. A single transcriptional start site was identified 27 residues downstream of a putative TATA box. The 5′ end of the transcript was shown to contain a region rich in adenyl residues (27 of 35). This region would not be conducive to secondary structure formation, which raises the possibility thatCyp2 might be preferentially translated during stress conditions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00028878
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