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  • 1
    Electronic Resource
    Electronic Resource
    Distribution of SchistoFLRFamide-like immunoreactivity in the adult ventral nervous system of the locust, Schistocerca gregaria (1995)
    Springer
    Cell & tissue research 281 (1995), S. 339-348 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Insect ; Nervous system ; insect ; Neuropeptides ; SchistoFLRFamide ; Schistocerca gregaria (Insecta)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. SchistoFLRFamide (PDVDHVFLRF-NH2) is one of the major endogenous neuropeptides of the FMRF-amide family found in the nervous system of the locust, Schistocerca gregaria. To gain insights into the potential physiological roles of this neuropeptide we have examined the distribution of SchistoFLRFamide-like immunoreactivity in the ventral nervous system of adult locusts by use of a newly developed N-terminally specific antibody. SchistoFLRFamide-like immunoreactivity in the ventral nerve cord is found in a subgroup of the neurones that are immunoreactive to an antiserum raised against bovine pancreatic polypeptide (BPP). In the suboesophageal ganglion three groups of cells stain, including one pair of large posterior ventral cells. These cells are the same size, in the same location in the ganglion and have the same branching pattern as a pair of BPP immunoreactive cells known to innervate the heart and retrocerebral glandular complex of the locust. In the thoracic and abdominal ganglia two and three sets of cells, respectively, stain with both the SchistoFLRFamide and BPP antisera. In the abdominal ganglia the immunoreactive cells project via the median nerves to the intensely immunoreactive neurohaemal organs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050430
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  • 2
    Electronic Resource
    Electronic Resource
    An FMRFamide antiserum differentiates between populations of antigens in the brain and retrocerebral complex of the locust, Schistocerca gregaria (1987)
    Springer
    Cell & tissue research 250 (1987), S. 93-99 
    Details
    ISSN: 1432-0878
    Keywords: Nervous system ; Immunocytochemistry ; FMRFamide ; Bovine pancreatic polypeptide ; Insects ; Schistocerca gregaria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of FMRFamide-irmunoreactive cell bodies in the brain and retrocerebral complex of the locust, Schistocerca gregaria, is described. Most of the immunoreactive cell bodies are found in the pars intercerebralis and in the optic lobes. Many, but not all, of the cell bodies also react with an antiserum raised against bovine pancreatic polypeptide, but this antiserum also reveals another population of cells that stain selectively with this antiserum. In addition to the cell bodies, numerous immunoreactive processes are revealed by both antisera in neuropilar regions of the brain. The results of blocking experiments suggest that a differential distribution of three locust antigens can be determined from the examination of alternate serial sections stained with the two antisera used.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00214659
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  • 3
    Electronic Resource
    Electronic Resource
    An FMRFamide antiserum differentiates between populations of antigens in the ventral nervous system of the locust, Schistocerca gregaria (1985)
    Springer
    Cell & tissue research 242 (1985), S. 109-114 
    Details
    ISSN: 1432-0878
    Keywords: Nervous system ; Insects ; Immunocytochemistry ; FMRFamide ; Bovine pancreatic polypeptide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of FMRFamide immunoreactive neurones in the ventral nerve cord of the locust, Schistocerca gregaria, is described. These neurones are found only in the suboesophagael and thoracic ganglia, although immunoreactive processes are found in the neuropils of the abdominal ganglia. Many of these neurones also react with an antiserum raised against bovine pancreatic polypeptide (BPP), but this antiserum also reveals another population of cells in the abdominal ganglia. The staining obtained with the BPP antiserum is blocked by preabsorption of the antiserum with FMRFamide; the converse is not true: FMRFamide-like immunoreactivity is not suppressed by preincubation with BPP. These results suggest that there are at least two endogenous peptide antigens in the locust nerve cord: one is found in cells of the suboesophageal and thoracic ganglia, and the other is found in cells of the abdominal ganglia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225568
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