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  • 1
    Electronic Resource
    Electronic Resource
    Chromosome-mediated transfer of murine alleles for hypoxanthine-guanine phosphoribosyl transferase (HGPRT) and ouabain resistance into human cell lines (1985)
    Springer
    Biochemical genetics 23 (1985), S. 1-15 
    Details
    ISSN: 1573-4927
    Keywords: chromosome-mediated gene transfer ; hypoxanthine-guanine phosphoribosyl transferase (HGPRT) ; ouabain resistance ; HT 1080 fibrosarcoma cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Genetic drug-resistance markers were transferred via purified metaphase chromosomes from mouse L cells into the human fibrosarcoma line HT1080 and HeLa S3 cells. Interspecific chromosome-mediated transfer of hypoxanthine-guanine phosphoribosyl transferase (HGPRT; EC 2.4.2.8) from mouse L cells into HGPRT− HT1080 cells occurred at a frequency of approximately 1×10−7. The presence of the mouse allele for HGPRT in transferent isolates was confirmed by isoelectric focusing. Transfer of ouabain resistance from mouse L cells to HT1080 and HeLa S3 cells occurred at an average frequency of approximately 4×10−7. Expression of the mouse trait in transferent isolates was confirmed by their ability to withstand doses of ouabain which would be lethal to spontaneous ouabain-resistant mutants of the human cells but not to mouse L cells. Ouabain-resistant transferents of human cells showed 104- to 〉105-fold enhanced drug resistance, characteristic of either wild-type or mutant alleles, respectively, from ouabain-resistant donor L cells. Unstable expression of the transferred phenotypes in the absence of selection was seen in some isolates, but expression was lost at slow rates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00499108
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  • 2
    Electronic Resource
    Electronic Resource
    Soldier-specific chemicals of the termiteCurvitermes strictinasus Mathews (Isoptera, Nasutitermitinae) (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 127-133 
    Details
    ISSN: 1573-1561
    Keywords: Isoptera ; Nasutitermitinae ; isolation ; identification ; Curvitermes strictinasus (Mathews) ; defense secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The defense secretion of the soldier termites ofCurvitermes strictinasus Mathews has been analyzed. Seven components have been isolated and identified, including limonene, terpinolene,p-cymen-8-ol, tridecan-2-one, tridecen-2-onecis,trans-farnesal, andtrans,trans-farnesal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00988640
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  • 3
    Electronic Resource
    Electronic Resource
    Chemical composition of the frontal gland secretion ofSyntermes soldiers (Isoptera, Termitidae) (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 135-145 
    Details
    ISSN: 1573-1561
    Keywords: Isoptera ; Nasutitermitinae ; Syntermes species ; defensive secretion ; isolation ; identification ; terpenoid compounds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The defensive secretions from the frontal glands of soldier termites of the genusSyntermes contain similar mixtures of mono- and sesquiterpene hydrocarbons. The major components inS. dirus, S. molestus, S. brevimalatus, S. peruanus, and a new species (Syntermes sp. n), iscis-β-ocimene. A substantial amount of aristolochene is found inSyntermes sp. n. and is present at lower levels in all the other species;S. brevimalatus contains onlycis-β-ocimene and aristolochene. The four other species also contain minor amounts of epi-α-selinene and germacrene A. The latter compound has been identified on the basis of its rearrangement product β-elemene. The termiteS. grandis differed markedly from the otherSyntermes species in that no terpenoid components were found in the soldier extract. With the obvious exception ofS. grandis, the same soldier-specific mono- and sesquiterpenes occurred in all species. The total amount of secretion per unit weight of soldiers varies with the species and is inversely proportional to the development of the mandibular apparatus. InS. molestus smaller gland size is compensated for by a greater number of soldiers foraging trails.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00988641
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