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  • 1
    Electronic Resource
    Electronic Resource
    In vitro culture of isolated microspores and regeneration of plants in Brassica campestris (1992)
    Springer
    Plant cell reports 11 (1992), S. 234-237 
    Details
    ISSN: 1432-203X
    Keywords: Brassica campestris ; Microspore ; Embryogenesis ; Haploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A protocol previously developed for B. napus microspore culture was modified to produce embryos from several lines of Brassica campestris. Bud size, genotype, media constituents, and incubation time and temperature were examined. Donor plants were grown in a growth cabinet at a day/night temperature of 10/5°C. Microspores were isolated from buds 2.0 – 2.9 mm in length and cultured in modified Lichter (1982) medium containing 17% sucrose, pH 6.2. After 48 h at 32°C, the incubation medium was replaced with NLN (Lichter 1982) medium containing 10% sucrose. Microspores were cultured at 24°C in darkness and embryos developed after three weeks. More than 1000 plants have thus far been regenerated. Genotypic differences were observed for microspore embryogenesis. The majority of the regenerants were haploid, however colchicine could be effectively used to achieve chromosome doubling.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00235072
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  • 2
    Electronic Resource
    Electronic Resource
    Evaluation of Brassica rapa L. genotypes for microspore culture response and identification of a highly embryogenic line (1995)
    Springer
    Plant cell reports 14 (1995), S. 580-584 
    Details
    ISSN: 1432-203X
    Keywords: Brassica rapa ; Microspore ; Embryogenesis ; Haploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isolated microspore culture techniques are being widely used in Brassica breeding programs to generate haploid and doubled haploid plants. A number of factors influence regeneration response in vitro including genotype. In order to assess the effect of genotype on microspore embryogenesis in B. rapa L. var. oleifera, 17 cultivars and breeding lines were evaluated. Embryos developed from all but one genotype when using NLN medium with 17% sucrose, followed by a reduction in sucrose concentration to 10%, 48 h later. The number of embryos /100 buds differed between genotypes, ranging from 0 to 70. Further studies indicated that sucrose concentration and incubation time influenced embryogenesis. Selection studies carried out with an Agriculture and Agri-Food Canada breeding line have resulted in the identification of a highly embryogenic B. rapa line. This line produced thousands of microspore-derived embryos /100 buds and will be useful in mutant selection and gene transfer as well as biochemical and developmental studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231942
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  • 3
    Electronic Resource
    Electronic Resource
    Stimulation of embryogenesis and haploid production in Brassica campestris anther cultures by elevated temperature treatments (1979)
    Springer
    Theoretical and applied genetics 55 (1979), S. 65-67 
    Details
    ISSN: 1432-2242
    Keywords: Brassica campestris ; Embryogenesis ; Haploid ; Microspore ; Temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Culture of Brassica campestris anthers at 35°C for one or three days prior to culture at 25°C significantly stimulated the yield of microspore-derived embryos. More than 100 plants were regenerated from cultured embryos and haploids were identified amongst them. The haploid frequency was greater than 70% if all small-flowered sterile plants were considered to be haploid. The yield of microspore-derived plants in B. campestris is approaching the level where anther culture may be utilized as a practical breeding tool.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285191
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