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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 137 (1984), S. 236-240 
    ISSN: 1432-072X
    Schlagwort(e): Desulfovibrio vulgaris ; Dissimilatory sulfate reduction ; Growth yields ; Chemostat cultures ; Pyruvate metabolism ; ATP synthesis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Desulfovibrio vulgaris (strain Marburg) was grown on H2 and sulfate as sole energy source in a chemostat limited by the sulfate supply. The biomass concentration and the sulfate concentration in the culture were determined as a function of the dilution rate. From the data a K S (saturation constant) for sulfate of 10 μM, a μmax of 0.23 h−1, and a $${\text{Y}}_{{\text{SO}}_{\text{4}} ^{2 - } }^{{\text{max}}}$$ of 13 g/mol were calculated. The organism was also grown in chemostat culture on H2 and sulfite, H2 and thiosulfate, and pyruvate (without sulfate). $${\text{Y}}_{{\text{SO}}_3 ^{2 - } }^{{\text{max}}}$$ was found to be 35 g/mol, $${\text{Y}}_{{\text{S}}_{\text{2}} {\text{O}}_{\text{3}} ^{2 - } }^{{\text{max}}}$$ 36 g/mol, and Y pyr max 10 g/mol. The growth yields are discussed with respect to ATP gains in dissimilatory sulfate reduction.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 116 (1978), S. 41-49 
    ISSN: 1432-072X
    Schlagwort(e): Desulfovibrio ; Chemolithotrophic growth ; H2-Oxidation ; Sulfate-reduction ; Growth yields ; Cell carbon synthesis ; Acetate assimilation ; Desulfoviridin ; Cytochrome c3
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Two sulfate reducing bacteria (Madison and Marburg strains) that grew on H2 plus sulfate in a mineral salts medium that contained acetate and CO2 as sole carbon source were isolated from diverse environments. During growth in this medium 4.2 mol of H2 were consumed per mol of sulfate reduced to sulfide. Acetate was required for biosynthetic purposes only. Approximately 70% of the cell carbon synthesized was derived from acetate and 30% from CO2. Acetate was not involved in dissimilatory sulfate reduction. Growth of the bacteria on H2 plus sulfate was linear rather than exponential, and a doubling time at the beginning of linear growth of approximately 3 h was observed. The optimal growth temperature was found to be near 35° C. Cultures could be grown up to a density of 500 mg cells (dry weight) per liter. Growth yield studies demonstrated that between 4 and 5 g of cells (dry weight) were formed per mol of sulfate reduced to sulfide. The chemolithotrophically growing sulfate reducing isolates were identified as Desulfovibrio species by being obligately anaerobic, gram negative, non spore forming vibrios that contained desulfoviridin and cytochrome c3 (350–450 nmol/g protein). The organisms were found to be monopolarly and monotrichously flagellated. The abilities of the two strains to grow on electron donors other than H2 and to use electron acceptors other than sulfate differed considerably. The DNA base composition of the Madison and Marburg strains were 60 and 63.5 mol % GC, respectively. The taxonomic status of the strains was discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    ISSN: 1432-072X
    Schlagwort(e): Desulfovibrio ; Chemolithothrophic growth ; H2 oxidation ; Sulfate reduction ; Thiosulfate reduction ; Growth rates ; Growth yields ; Maintenance coefficients ; Y ATP max
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Desulfovibrio vulgaris (Marburg) was grown on H2 plus sulfate and H2 plus thiosulfate as the sole energy sources and acetate plus CO2 as the sole carbon sources. Conditions are described under which the bacteria grew exponentially. Specific growth rates (μ) and molar growth yields (Y) at different pH were determined. μ and Y were found to be strongly dependent on the pH. Highest growth rates and molar growth yields were observed for growth on H2 plus sulfate at pH 6.5 (μ=0.15h-1; Y SO 4 2- =8.3g·mol-1) and for growth on H2 plus thiosulfate at pH 6.8 (μ=0.21h-1; Y S 2O 3 2 =16.9g·mol-1). The growth yields were found to increase with increasing growth rates: plots of 1/Y versus 1/μ were linear. Via extrapolation to infinite growth rates a Y SO4 2- /max of 12.2g·mol-1 and a YS2O 3 2- /max of 33.5g·mol-1 was obtained. The growth yield data are interpred to indicate that dissimilatory sulfate reduction to sulfide is associated with a net synthesis of 1 mol of ATP and that near to 3 mol of ATP are formed during dissimilatory sulfite reduction to sulfide.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 127 (1980), S. 59-65 
    ISSN: 1432-072X
    Schlagwort(e): Methanobacterium thermoautotrophicum ; Growth rates ; Growth yields ; Nickel ; Maintenance coefficient ; Interspecies hydrogen transfer ; Saturation constants
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Methanobacterium thermoautotrophicum was grown on a mineral salts medium in a fermenter gassed with H2 and CO2, which were the sole carbon and energy sources. Under the conditions used the bacterium grew exponentially. The dependence of the growth rate (μ) on the concentration of H2 and CO2 in the incoming gas and the dependence of the growth yield ( $$Y_{CH_4 }$$ ) on the growth rate were determined at pH 7 (the pH optimum) and 65° C (the temperature optimum). The curves relating growth rate to the H2 and CO2 concentration were hyperbolic. From reciprocal plots apparent K s values for H2 and CO2 and μmax were obtained: app. $$K_{{\text{H}}_{\text{2}} }$$ = 20%; app. $$K_{{\text{CO}}_{\text{2}} }$$ = 11%; μ = 0.69 h-1; t δ (max)=1 h. $$Y_{CH_4 }$$ was 1.6 g mol-1 and almost independent of the growth rate, when the rate of methane formation was not limited by the supply of either H2 or CO2. The yield increased to near 3 g mol-1 when H2 or CO2 were limiting. These findings indicate that methane formation and growth are less tightly coupled at high concentrations of H2 or CO2 in the medium than at low concentrations. The physiological significance of these findings is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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