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  • 1
    Electronic Resource
    Electronic Resource
    Analysis of the parsley chalcone-synthase promoter in response to different light qualities (1994)
    Springer
    Planta 193 (1994), S. 275-282 
    Details
    ISSN: 1432-2048
    Keywords: Chalcone synthase ; Footprinting in vivo ; Gene expression (transient) ; Light regulation (UV-B photoreceptor, blue-light photoreceptor) ; Petroselinum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We examined the chalcone synthase (chs) promoter from parsley [Petroselinum crispum Miller (A.W. Hill)] for the existence of separate promoter elements responsible for transcriptional activation of the chs gene by UV-B and by blue light. A combination of in-vivo footprinting in parsley cells and light-induced transient expression assays with different chs promoter constructs in parsley protoplasts was used. Dark controls and bluelight-irradiated cells gave identical in-vivo footprints on the chs promoter. Pre-irradiation with blue light prior to a UV-B-light pulse is known to cause a shift in the timing of UV-B-light-induced increase in chs transcription rates. This shift was also manifested on the DNA template, since UV-B-light-induced in-vivo footprints in cells pretreated with blue light were detected earlier than in cells which had been irradiated with a UV-B-light pulse only. Although there was a clear shift in the timing of footprint appearance, the patterns of footprinting did not change. Light-induced transient-expression assays revealed that the shortest tested chs promoter which retained any light responsiveness, was sufficient for mediating both induction by UV light and the blue-light-mediated kinetic shift. These findings argue against a spatial separation of UV-B- and blue-light-responsive elements on the chs promoter. We interpret these data by postulating that the signal transduction pathways originating from the excitation of UV-B- and blue-light receptors merge at the chs promoter, or somewhere between light perception and protein-DNA interaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00192541
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    Paper (German National Licenses)
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  • 2
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    Titan Montgolfiere Balloon Analysis and Design using Computational Fluid Dynamics Simulations (2012)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: No abstract available
    Keywords: Fluid Mechanics and Thermodynamics
    Type: 9th International Planetary Probe Workshop (IPPW-9); Jun 18, 2012 - Jun 22, 2012; Toulouse; France
    Format: text
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    NASA TECHNICAL REPORTS
  • 3
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    Overview of a Proposed Flight Validation of Aerocapture System Technology (2006)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: Aerocapture is a very useful capability for NASA that can be used across a wide range of planetary mission sizes and destinations. A substantial mass advantage may be realized through aerocapture maneuver implementation. The mass advantage is enabling for certain outer planet mission profiles. Aerocapture technology provides corollary benefits to the related applications of atmospheric entry and precision landing on worlds with atmospheres through aero/aerothermodynamic model validation, hypersonic guided flight, tps materials, and performance model validation. The ST9 Aerocapture flight validation will be sufficient to immediately infuse aerocapture technology into future NASA science missions. The advanced technologies being flight validated will enable the system level goal of performing an aerocapture maneuver. The advanced technologies include: The GN&C System, TPS materials, plus Advanced recession and heat flux sensors.
    Keywords: Fluid Mechanics and Thermodynamics
    Type: M09-0147 , 42nd AIAA/ASME/SAE/ASEE Joint Propulsion Conference and Exhibit; Jul 10, 2006 - Jul 12, 2006; Sacramento, CA; United States
    Format: application/pdf
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    NASA TECHNICAL REPORTS
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