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  • 1
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    In:  rcowen@rsmas.miami.edu | http://aquaticcommons.org/id/eprint/14490 | 403 | 2014-02-13 04:26:39 | 14490 | United States National Marine Fisheries Service
    Publication Date: 2021-06-26
    Description: Plankton and larval fish sampling programs often are limited by a balance between sampling frequency (for precision) and costs. Advancements in sampling techniques hold the potential to add considerable efficiency and, therefore, add sampling frequency to improve precision. We compare a newly developed plankton imaging system, In Situ Ichthyoplankton Imaging System (ISIIS), with a bongo sampler, which is a traditional plankton sampling gear developed in the 1960s. Comparative sampling was conducted along 2 transects ~30–40 km long. Over 2 days, we completed 36 ISIIS tow-yo undulations and 11 bongo oblique tows, each from the surface to within 10 m of the seafloor. Overall, the 2 gears detected comparable numbers of larval fishes, representing similar taxonomic compositions, although larvae captured with the bongo were capable of being identified to lower taxonomic levels, especially larvae in the small (〈5 mm), preflexion stages. Size distributions of the sampled larval fishes differed considerably between these 2 sampling methods, with the size range and mean size of larval fishes larger with ISIIS than with the bongo sampler. The high frequency and fine spatial scale of ISIIS allow it to add considerable sampling precision (i.e., more vertical sections) to plankton surveys. Improvements in the ISIIS technology (including greater depth of field and image resolution) should also increase taxonomic resolution and decrease processing time. When coupled with appropriate net sampling (for the purpose of collecting and verifying the identification of biological samples), the use of ISIIS could improve overall survey design and simultaneously provide detailed, process-oriented information for fisheries scientists and oceanographers.
    Keywords: Biology ; Ecology ; Fisheries
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 1-12
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  • 2
    Publication Date: 2021-07-12
    Description: The identification of sea bass (Centropristis) larvae to species is difficult because of similar morphological characters, spawning times, and overlapping species ranges. Black sea bass (Centropristis striata) is an important fishery species and is currently considered to be overfished south of Cape Hatteras, North Carolina. We describe methods for identifying three species of sea bass larvae using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) assays based on species-specific amplification of rDNA internal transcribed spacer regions. The assays were tested against DNA of ten other co-occurring reef fish species to ensure the assay's specificity. Centropristis larvae were collected on three cruises during cross-shelf transects and were used to validate the assays. Seventy-six Centropristis larva were assayed and 69 (91%) were identified successfully. DNA was not amplified from 5% of the larvae and identification was inconclusive for 3% of the larvae. Those assays can be used to identify sea bass eggs and larvae and will help to assess spawning locations, spawning times, and larval dispersal.
    Keywords: Ecology ; Fisheries ; Chemistry ; sea basses ; Centropristis spp. ; genetic identification
    Repository Name: AquaDocs
    Type: article
    Format: application/pdf
    Format: application/pdf
    Format: 183-193
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  • 3
    Publication Date: 2021-07-12
    Description: We analyzed the relationships between the larval and juvenile abundances of selected estuarine-dependent fishes that spawn during the winter in continental shelf waters of the U.S. Atlantic coast. Six species were included in the analysis based on their ecological and economic importance and relative abundance in available surveys: spot Leiostomus xanthurus, pinfish Lagodon rhomboides, southern flounder Paralichthys lethostigma, summer flounder Paralichthys dentatus, Atlantic croaker Micropogonias undulatus, and Atlantic menhaden Brevoortia tyrannus. Cross-correlation analysis was used to examine the relationships between the larval and juvenile abundances within species. Tests of synchrony across species were used to find similarities in recruitment dynamics for species with similar winter shelf-spawning life-history strategies. Positive correlations were found between the larval and juvenile abundances for three of the six selected species (spot, pinfish, and southern flounder). These three species have similar geographic ranges that primarily lie south of Cape Hatteras. There were no significant correlations between the larval and juvenile abundances for the other three species (summer flounder, Atlantic croaker, and Atlantic menhaden); we suggest several factors that could account for the lack of a relationship. Synchrony was found among the three southern species within both the larval and juvenile abundance time series. These results provide support for using larval ingress measures as indices of abundance for these and other species with similar geographic ranges and winter shelf-spawning life-history strategies.
    Keywords: Ecology ; Management ; Fisheries ; North Carolina
    Repository Name: AquaDocs
    Type: article
    Format: application/pdf
    Format: application/pdf
    Format: 12-21
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  • 4
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    In:  http://aquaticcommons.org/id/eprint/2111 | 403 | 2014-02-18 18:49:34 | 2111 | United States National Marine Fisheries Service
    Publication Date: 2021-07-12
    Description: Patterns were investigated in juvenile fish use of unconsolidated sediments on the southeast United States continental shelf off Georgia. Juvenile fish and environmental data were sampled at ten stations along a110-km cross-shelf transect, including four stations surrounding Gray’s Reef National Marine Sanctuary (Gray’sReef NMFS). Cross-shelf stations were sampled approximately quarterly from spring 2000 to winter 2002. Additional stations were sampled on three transects inshore of Gray’s Reef NMS and four transects offshore of the Sanctuary during three cruises to investigate along-shelf patterns inthe juvenile fish assemblages. Samples were collected in beam trawls, and 121 juvenile taxa, of which 33 were reef-associated species, were identified. Correspondence analysison untransformed juvenile fish abundance indicated a cross-shelf gradient in assemblages, and the station groupings and assemblages varied seasonally. During the spring,fall, and winter, three cross-shelf regions were identified: inner-shelf, mid-shelf, and outer-shelf regions. In the summer, the shelf consisted of a single juvenile fish assemblage. Water depth was the primary environmental variable correlated with cross-shelfassemblages. However, salinity, density, and water column stratification also correlated with the distributionof assemblages during the spring, fall, and winter, and along with temperature likely influenced the distributionof juvenile fish. No along-shelf spatial patterns were found in the juvenile fish assemblages, but the along-shelf dimension sampled was small (~60 km). Our results revealedthat a number of commercially and recreationally important species used unconsolidated sediments on the shelf off Georgia as juvenile habitat. We conclude that management efforts would be improved through a greater recognition of the importance of these habitats to fish production and theinterconnectedness of multiple habitats in the southeast U.S. continental shelf ecosystem.
    Keywords: Ecology ; Management ; Fisheries
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 256-277
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