ISSN:
1432-2242
Schlagwort(e):
Key words Wheat
;
Triticum aestivum
;
Transformation
;
Nuclear male sterility
;
DNA-integration
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract Nuclear male sterility within Triticum aestivum is considered as the ideal basis for the development of a hybridization system for wheat. We engineered nuclear male sterility in wheat by introducing the barnase gene under the control of tapetum-specific promoters derived from corn and rice. A biolistic-mediated transformation method, based on the use of the poly(ADP-ribose)polymerase inhibitor niacinamide, was set up which enriched for low-copy integrations (1–3 copies). Most of these copies were not linked and segregated in the next generation.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/s001220050540
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