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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 41 (1986), S. 231-235 
    ISSN: 1570-7458
    Keywords: genetic sexing ; Ceratitis capitata ; field cages ; competitiveness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Des expériences en cage en champ ont été réalisées en Italie avec des populations naturelles de Ceratitis capitata en utilisant une lignée dont le sexage était basé sur le dimorphisme de la couleur des pupes. Les expériences ont été conçues pour contrôler la compétitivité des mâles de cette lignée face aux mâles sauvages, et d'évaluer l'effet du retrait des femelles avant le lâcher sur le pourcentage de femelles sauvages accouplées avec des mâles lâchés. Les expériences montrent que la compétition ne défavorise pas la lignée génétiquement sexable mais révèlent une fréquence élevée d'accouplement intralignée. Le retrait des femelles avant le lâcher modifie radicalement l'orientation des accouplements d'intra vers interlignées. Ce changement accroît fortement l'efficacité de la technique d'utilisation des mâles stériles dans la lutte biologique. On en a conclu que non seulement des intérêts économiques évidents mais aussi des avantages biologiques significatifs seraient retirés de l'intégration de telles lignées sexables génétiquement dans les programmes de lutte avec des mâles stériles.
    Notes: Abstract Using a genetic sexing line based on pupal colour dimorphism in Ceratitis capitata Wied., field cage experiments were carried out with a wild population in Italy. The experiments were designed to monitor the competitiveness of males of this line in comparison with wild males and to assess the effect of removal of females prior to release on the proportion of wild females mated by the released males. The results showed that the genetic sexing strain competed well with the wild population but there was a large degree of intra-strain mating. Removal of females prior to release radically changed the mating pattern from intra to inter-strain mating. This change greatly increases the efficiency of the sterile insect method of control. It was concluded that as well as the obvious economic advantages of genetic sexing, significant biological advantages would also be associated with the integration of such a strain in a sterile insect control programme.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Biochemical genetics 24 (1986), S. 765-774 
    ISSN: 1573-4927
    Keywords: Ceratitis capitata ; alcohol dehydrogenase ; null mutants ; genetic sexing ; Y-linked translocations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Alcohol dehydrogenase null mutants have been induced with X rays in Ceratitis capitata, for use in a genetic sexing system. A combination of selective medium and electrophoretic screening was used to identify these mutants. The results indicate that they are probably large deletions since no homozygotes were found for any of the induced mutants.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 72 (1986), S. 455-457 
    ISSN: 1432-2242
    Keywords: Genetic sexing ; ADH ; Allyl alcohol ; Ceratitis capitata ; Medfly
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using the alcohol dehydrogenase (ADH) locus a genetic sexing system is being developed in the Mediterranean fruit fly Ceratitis capitata based on the sensitivity of ADH null mutations to environmental ethanol. A series of null mutants have been induced at this locus, however, none proved viable as homozygotes. One of these null mutants was translocated to the male determining chromosome and this line can be used for genetic sexing. When larvae from this line were reared on larval medium containing various concentrations of allyl alcohol, 97% of the emerging adults were males; in the absence of the allyl alcohol the sex ratio in the line is distorted in favour of the females. It is proposed that the higher ADH activity of the females (homozygous positive) in comparison with the males (heterozygous null) is responsible for their lower survival in larval medium containing allyl alcohol. ADH converts the allyl alcohol to the lethal ketone. The possible use of this line to sex large populations of medflies for use in sterile insect release programmes is discussed.
    Type of Medium: Electronic Resource
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