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  • Capillary  (1)
  • Intracellular structure  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    A vascular network closely linked to the epithelium of the urinary bladder of the rat (1991)
    Springer
    Cell & tissue research 263 (1991), S. 137-143 
    Details
    ISSN: 1432-0878
    Keywords: Urinary bladder ; Capillary ; Urothelium ; Vascularization ; Rat (Sprague-Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Scanning electron microscopy was used on the mucosa of the rat urinary bladder after digestion with strong alkali and microdissection. The underside of the epithelium (and the plane of the epithelium-tunica propria interface) is not smooth but is scored by grooves-10 μm wide and 3–4 μm deep—connected into a fine mesh. A net of blood capillaries located in the uppermost part of the tunica propria occupies these grooves. They measure 3–9 μm in diameter, are separated from the epithelium by a gap of 0.3 μm, often show fenestrations, and are accompanied by numerous and extensive pericytes and by some fibroblasts. We discuss these observations in the light of current knowledge of blood flow in the bladder, contraction and distension of the bladder wall and formation of mucosal folds, transport of solutes through the epithelium, and plasma extravasation from mucosal blood vessels in neurogenic inflammation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318409
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  • 2
    Electronic Resource
    Electronic Resource
    An application of rapid freezing, freeze substitution-fixation for scanning electron microscopy (1985)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 2 (1985), S. 201-208 
    Details
    ISSN: 0741-0581
    Keywords: SEM ; Rapid freezing ; Osmium ; Dimethyl sulfoxide ; Intracellular structure ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: A combined technique of the rapid freezing, freeze substitution-fixation method and the osmium-DMSO-osmium method was devised. By this combined method we clearly observed the architecture of intracellular components in three dimensions. Morphological characteristics were generally similar to those of tissue prepared by the osmium-DMSO-osmium method but different in some respects. Mucigen droplets in intestinal goblet cells, for example, appeared as separated spheres, while in specimens prepared by chemical fixation they were observed as a mass of fused droplets. In the Golgi complex, all cisternae were extremely flat, although they usually dilated on the cis side after chemical fixation. Particles on the mitochondrial tubules of liver cells were well distinguished. They were mushroom shaped, as are those observed by negative staining. The combined method, that is, the rapid freezing, osmium-DMSO-osmium method, is thought to be effective for studying the true structure of intracellular components by scanning electron microscopy.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060020305
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