ISSN:
0306-042X
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Two isotope dilution mass spectrometric methods have been developed for the determination of D-glucose in human serum. Each uses a uniformly labeled (13C)glucose as the internal standard. The first method involves conversion of glucose into 1,2: 5,6-di-O-isopropylidene-α-D-glucofuranose and an extensive clean-up, followed by quantitation using packed column gas chromatography mass spectrometry. In the second method, glucose is converted into α-D-glucofuranose cyclic 1,2: 3,5-bis(butylboronate)-6-acetate. The wet chemistry work-up is simpler, but analysis by capillary gas chromatography mass spectrometry is required. Both methods exhibit excellent precision (coefficients of variation 〈0.3%) and provided mean values that agree within 1% for all serum pools tested.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200090907
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