ISSN:
1573-5001
Schlagwort(e):
4D NMR
;
deuteration
;
high molecular weight proteins
;
protein assignment
;
triple resonance NMR
;
TROSY
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Chemie und Pharmazie
Notizen:
Abstract A 4D TROSY-based triple resonance experiment, 4D-HNCOi−1CAi, is presented which correlates intra-residue 1HN, 15N, 13 Cα chemical shifts with the carbonyl (13C′) shift of the preceding residue. The experiment is best used in concert with recently described 4D TROSY-HNCOCA and -HNCACO experiments [Yang, D. and Kay, L.E. (1999) J. Am. Chem. Soc., 121, 2571–2575]. In cases where degeneracy of (1HN,15N) spin pairs precludes assignment using the HNCOCA and HNCACO, the HNCOi−1CAi often allows resolution of the ambiguity by linking the 13Cα and 13C′ spins surrounding the (1HN,15N) pair. The experiment is demonstrated on a sample of 15N, 13C, 2 H labeled maltose binding protein in complex with β-cyclodextrin that tumbles with a correlation time of 46 ns.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1023/A:1008310617047
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