Publication Date:
1999-05-15
Description:
Coinjection of unfertilized mouse oocytes with sperm heads and exogenous DNA encoding either a green fluorescent protein (GFP) or beta-galactosidase reporter produced 64 to 94 percent transgene-expressing embryos, reflecting DNA-sperm head association before coinjection. Nonselective transfer to surrogate mothers of embryos in the GFP series generated about 20 percent offspring expressing the integrated transgene. These data indicate that exogenous DNA can reproducibly be delivered into an oocyte by microinjected spermatozoa and suggest an adaptable method of transgenesis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Perry, A C -- Wakayama, T -- Kishikawa, H -- Kasai, T -- Okabe, M -- Toyoda, Y -- Yanagimachi, R -- HD-34362/HD/NICHD NIH HHS/ -- New York, N.Y. -- Science. 1999 May 14;284(5417):1180-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, HI 96822, USA. perry@hawaii.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10325231" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Blastocyst/physiology
;
Cell Membrane/physiology
;
Culture Techniques
;
Embryo Transfer
;
Embryonic and Fetal Development
;
Female
;
*Fertilization in Vitro
;
*Gene Transfer Techniques
;
Genes, Reporter
;
Green Fluorescent Proteins
;
Lac Operon
;
Luminescent Proteins/genetics
;
Male
;
Mice
;
*Mice, Transgenic/embryology/genetics
;
Microinjections
;
Morula/physiology
;
Oocytes
;
Sperm Head/*physiology
;
*Transgenes
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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