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  • 1
    Electronic Resource
    Electronic Resource
    Cryopreservation of zygotic embryos of a Japanese terrestrial orchid (Bletilla striata) by vitrification (1997)
    Springer
    Plant cell reports 16 (1997), S. 754-757 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsBletilla striata ; Cryopreservation ; Embryo ; Orchid ; Vitrification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The seeds of a Japanese terrestrial orchid (Bletilla striata Rchb.f.) were germinated and cultured on solidified new Dogashima (ND) medium for 10 days. These embryos were then precultured on ND medium supplemented with 0.3 m sucrose for 3 days at 25°C in continuous dark. The embryos were then overlaid with a mixture of 2 m glycerol and 0.4 m sucrose for 15 min at 25°C and finally dehydrated with highly concentrated vitrification solution (PVS2) for 3 h at 0°C prior to immersion into liquid nitrogen for 30 min. After rapid warming, the embryos were washed with liquid ND medium supplemented with 1.2 m sucrose for 20 min and then plated on ND medium. Successfully vitrified and warmed embryos developed into normal plantlets. The rate of plant regeneration amounted to about 60%. This vitrification method appears to be a promising technique for cryopreservation of orchids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050314
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  • 2
    Electronic Resource
    Electronic Resource
    In vitro propagation, restriction fragment length polymorphism, and random amplified polymorphic DNA analyses of Angelica plants (1998)
    Springer
    Plant cell reports 18 (1998), S. 187-192 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsAngelica ; Restriction fragment length polymorphism ; Random amplified polymorphic DNA ; Ligustilide ; In vitro propagation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Angelica acutiloba, a medicinal plant used as a natural medicine Touki, was clonally propagated through axillary buds in vitro. No substantial differences were found in the random amplified polymorphic DNA (RAPD) pattern between the original A. acutiloba and the plant propagated in vitro, suggesting no changes in the DNA sequences and structure during in vitro propagation. The genetic similarities of several Angelica plants were investigated by restriction fragment length polymorphism (RFLP) and RAPD analyses. The RFLP and RAPD patterns of A. sinensis Diels were substantially different from those of A. acutiloba. Using ten different restriction enzymes, no RFLP was observed in the varieties of A. acutiloba. By RAPD analysis, A. acutiloba varieties can be classified into two major subgroups, i.e., A. acutiloba Kitagawa and A. acutiloba Kitagawa var. sugiyamae Hikino. The varieties of A. acutiloba Kitagawa in Japan and Angelica spp. in northeast China exhibited a very close genetic relationship.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050554
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation and identification of littorine from hairy roots of Atropa belladonna (1998)
    Springer
    Plant cell reports 18 (1998), S. 249-251 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsAtropa belladonna ; Hairy root ; Littorine ; Root culture ; Tropane alkaloid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A hairy root clone (M8) of Atropa belladonna, producing high levels of tropane alkaloids, was established by transformation with Agrobacterium rhizogenes (MAFF 03-01724). Littorine, an intermediate of tropane alkaloids, was detected by high-performance liquid chromatography and gas chromatography-mass spectrometry in the alkaloid fraction of the hairy roots and identified by nuclear magnetic resonance analysis. Littorine was also detected in the non-transformed root culture of A. belladonna.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050565
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  • 4
    Electronic Resource
    Electronic Resource
    High forskolin production in hairy roots of Coleus forskohlii (1998)
    Springer
    Plant cell reports 17 (1998), S. 457-459 
    Details
    ISSN: 1432-203X
    Keywords: Key words Forskolin ; Coleus forskohlii ; Hairy root
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hairy roots of Coleus forskohlii were induced by infection with the Agrobacterium rhizogenes MAFF 03-01724 strain. Growth and forskolin production of two hairy root clones cultured in various liquid media were examined. Hairy root clone B9 grew well in woody plant liquid medium and showed a high forskolin yield (ca. 1.3 mg/ 100 ml flask) after 5 weeks of culture. The time course of growth and forskolin production of the clone B9 cultured in woody plant liquid medium was also examined. Rapid growth started at week 2 and continued until week 5. The highest forskolin yield (ca. 1.6 mg/100 ml flask) was obtained at week 5. Productivity was much higher than that previously reported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050425
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  • 5
    Electronic Resource
    Electronic Resource
    Production of tropane alkaloids by transformed root cultures of Atropa belladonna in stirred bioreactors with a stainless steel net (1999)
    Springer
    Plant cell reports 18 (1999), S. 567-571 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsAtropa belladonna ; Transformed root cultures ; Scale-up ; Tropane alkaloids ; Modified stirred bioreactors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A scale up of transformed root cultures of Atropa belladonna from a 300-ml flask to a 30-l tank was accomplished without any reduction in alkaloid productivity. Cutting treatment of seed cultures showed no distinct effect on root growth, morphology, and alkaloid content in conical flasks during 1 month of culture. Randomly cut roots thus grown were further cultivated in 3-l and 30-l modified stirred bioreactors for a scale-up culture. After 1 month of culture, 1490 mg of tropane alkaloids was produced by a 30-l culture of A. belladonna transformed roots. These roots contained the same level of atropine (5.4 mg/ g dw) as the roots of this plant grown in the field for 12 months and still contained a considerable amount of other alkaloids including 1.6 mg/g dw of 6-β-hydroxyhyoscyamine, 0.9 mg/g dw of scopolamine, and 2.0 mg/g dw of littorine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050623
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  • 6
    Electronic Resource
    Electronic Resource
    Kinetics of littorine content in various developing stages of regenerates of Atropa belladonna (2000)
    Springer
    Plant cell reports 19 (2000), S. 1021-1026 
    Details
    ISSN: 1432-203X
    Keywords: Key words Atropa belladonna ; Hairy root ; Littorine ; Root growth ; Tropane alkaloid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Aseptically propagated regenerates were cultivated in a hydroponic apparatus, a phytotron or in the field, and their growth and littorine contents were investigated. No littorine was detected in aseptic regenerates cultured on solidified Murashige and Skoog medium, nor was it found in leaves under the three conditions tested. In roots, it was common features to all three conditions tested that littorine increased dramatically after transplantation from culture tubes and was a major alkaloid up to week 4; subsequently the littorine contents varied depending on the cultivation conditions. Roots cultivated in the field showed a marked thickening and rapid disappearance of littorine; those cultivated in the hydroponic apparatus were thin and maintained a high level of littorine for a long time. In a plant cultivated for 16 weeks in a pot, littorine content in the roots decreased with increasing root diameter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000231
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  • 7
    Electronic Resource
    Electronic Resource
    Shikonin derivative formation on the stem of cultured shoots in Lithospermum erythrorhizon (2000)
    Springer
    Plant cell reports 19 (2000), S. 1121-1126 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsLithospermum erythrorhizon ; Shoot cultures ; Stem ; Shikonin derivatives ; Abbrevations B5: Gamborg B5 (Gamborg et al. 1968) ; HF: Phytohormone-free ; HPLC: High-performance liquid chromatography ; IAA: Indole-3-acetic acid ; IBA: Indole-3-butyric acid ; LS: Linsmaier and Skoog (1965) ; MS: Murashige and Skoog (1962) ; 1/2 MS: Half strength Murashige and Skoog (1962) ; NMR: Nuclear magnetic resonance ; RC: Root culture (Thomas and Davey 1982) ; SD: Standard deviation ; TLC: Thin-layer chromatography ; WP: Woody plant (Lloyd and McCown 1980)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Lithospermum erythrorhizon , which are capable of producing red pigments, have been established. The red pigments were formed on the stems of L. erythrorhizon shoots cultured both on solid and in liquid media without phytohormones at 25 °C in the dark. Thin-layer chromatography, high-performance liquid chromatography and 1 H nuclear magnetic resonance analyses revealed that the red pigments which accumulated on the cultured shoots were shikonin derivatives. The effects of various basal media and phytohormones (indole-3-acetic acid, indole-3-butyric acid and kinetin) on the growth and the formation of shikonin derivatives were investigated. When the shoots were cultured on Murashige and Skoog solid medium, the addition of kinetin remarkably enhanced shikonin derivative accumulation in the shoots. However, these effects of kinetin were not observed in the liquid culture when cultured in Gamborg B5 medium. The maximum content of shikonin derivatives (2.3% as dry weight, ca. 1.5 mg/100 ml flask) was observed in the shoots cultured in phytohormone-free B5 liquid medium for 5 weeks.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000237
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  • 8
    Electronic Resource
    Electronic Resource
    Factors affecting the in vitro rapid germination of Taxus embryos and the evaluation of taxol content in the plantlets (1994)
    Springer
    Plant cell, tissue and organ culture 39 (1994), S. 261-263 
    Details
    ISSN: 1573-5044
    Keywords: embryo culture ; Taxaceae ; yew
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A simple and efficient in vitro method for breaking the dormancy of Taxus baccata L. cv. Stricta seeds was investigated. The highest rate of germination (100%) of embryos isolated from seeds, which had been washed with running tap water for 7 days, was obtained after 7 days of culture on modified Murashige and Skoog or Heller media. The taxol equivalent content in 2-month-old Taxus plantlets was investigated using anti-taxol polyclonal antibodies. The results showed that the taxol equivalent content varied, depending on Taxus species and on the individuals in the same taxon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035979
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  • 9
    Electronic Resource
    Electronic Resource
    Development of muon polarization preserved scintillators (1997)
    Springer
    Hyperfine interactions 106 (1997), S. 291-293 
    Details
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We measured the residual μ+ polarizations in various liquid scintillators to search for an ideal material for an active muon stopper in which the muon spin polarization can be preserved and at the same time their energy loss and tracks can be measured by scintillation lights from the stopper material. We found the residual polarizations of mineral‐oil base liquid scintillators were \sim 4 times larger than that of a plastic scintillator. Further increase of the residual polarization was observed with a mixture of CCl4.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1012678916503
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  • 10
    Electronic Resource
    Electronic Resource
    Photon recycling transport in bulk GaAs probed by μSR with laser irradiation (1999)
    Springer
    Hyperfine interactions 120-121 (1999), S. 595-599 
    Details
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A newly developed μSR method combined with a tunable, high power and sharply pulsed laser was applied to GaAs. Anomalous transport phenomena of the photo excited effects into bulk crystal were observed, given that the stopping range of implanted muons is about 200 times larger than photo-absorption depth. In addition, the measurement of photon energy dependence of muon spin depolarization under laser irradiation suggests the impurity level associated with Mu BC 0 is located within the band gap of GaAs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1017055119248
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