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  • 1
    Electronic Resource
    Electronic Resource
    Molecular analysis of the nuclear organellar genotype of somatic hybrid plants between tomato (Lycopersicon esculentum) and Lycopersicon chilense (1992)
    Springer
    Plant cell reports 10 (1992), S. 629-632 
    Details
    ISSN: 1432-203X
    Keywords: Protoplast fusion ; RFLP ; Mitochondrial DNA ; Chloroplast DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Somatic hybrid plants were recovered following fusion of leaf mesophyll protoplasts isolated from tomato (Lycopersicon esculentum) cultivar UC82 with protoplasts isolated from suspension cultured cells of L. chilense, LA 1959. Iodoacetate was used to select against the growth of unfused tomato protoplasts. Two somatic hybrids were recovered in a population of 16 regenerants. No tomato regenerants were recovered; all of the non-hybrid regenerants were L. chilense. The L. chilense protoplast regenerants were tetraploid. The hybrid nature of the plants was verified using species-specific restriction fragment length polymorphisms for the nuclear, chloroplast and mitochondrial genomes. The somatic hybrids had inherited the chloroplast DNA of the tomato parent, and portions of the mitochondrial DNA of the L. chilense parent. The somatic hybrids formed flowers and developed seedless fruit.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232385
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Examination of genome stability in cultured Lycopersicon (1986)
    Springer
    Plant cell reports 5 (1986), S. 276-279 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genetic stability of plants regenerated from either mesophyll protoplasts or leaf slices of the F1 hybrid between Lycopersicon esculentum and L. pennellii was assayed by comparing the ploidy level, leaf morphology and isozyme patterns of the regenerants with their somatic parents. Regenerants from protoplasts were predominantly tetraploid, regenerants from leaf slices were predominantly diploid; both classes of regenerants had isozyme patterns identical to those of the parent plant. Callus was analyzed that grew up from cultures containing fused protoplasts from either irradiated or untreated protoplasts of L. esculentum and L. pennellii. The L. pennellii cell line used was 18 months old and could no longer regenerate. Out of 75 calli scored at 3 isozyme loci, 51 were heterozygous at only one or two of the loci. Irradiation of the two parental lines was not necessary to produce fusion products exhibiting asymmetric expression of parental genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269821
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  • 3
    Electronic Resource
    Electronic Resource
    Heat shock protein expression in thermotolerant and thermosensitive lines of cotton (1989)
    Springer
    Plant cell reports 8 (1989), S. 37-40 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The expression of heat shock proteins (HSPs) was compared between genetically characterized heat tolerant and heat sensitive lines of cotton (Gossypium hirsutum andG. barbadense) using electrophoretic analysis ofin vivo labelled proteins. No differences were observed between the two lines with regard to: the temperature at which HSP synthesis was induced (37°C); the temperature at which HSP synthesis was maximal (45°C); the rates of recovery from HSP synthesis; the duration of HSP synthesis; or the major size classes of HSPs expressed in these two lines. Several HSPs were identified on 2D gels which were expressed uniquely in either the tolerant or sensitive cotton line. However, the HSP pattern displayed in a heat tolerant BC-3 individual was that of the heat sensitive parent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00735774
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  • 4
    Electronic Resource
    Electronic Resource
    Structure and characterization of a putative drought-inducible H1 histone gene (1996)
    Springer
    Plant molecular biology 30 (1996), S. 255-268 
    Details
    ISSN: 1573-5028
    Keywords: abscisic acid ; diurnal cycling ; Lycopersicon spp.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A drought- and abscisic acid (ABA)-inducible gene, His1, was isolated from Lycopersicon pennellii, a drought-resistant relative of cultivated tomato, and the gene structure was defined experimentally. The nucleotide sequence of His1 predicts a protein of 202 amino acid residues, with a significant sequence homology to plant H1 histones. Consensus sequences for both H1 histone-specific promoter elements as well as an ABA-responsive element were identified in the 5′-flanking region of His1. Transcripts of this gene accumulate in leaf tissue in response to drought in three tomato species including cultivated tomato (L. esculentum), L. pennellii, and L. chilense, as well as in tobacco. Transcripts for His1 are constitutively expressed in roots; transcript abundances in tomato root tips were equivalent to transcript abundances in more mature regions of the seedling root. The accumulation in leaves of transcripts for His1 preceded visible symptoms of drought stress in the plants. Transcript accumulation was detected in both drought-sensitive and drought-resistant species at similar leaf water potentials, 255-1 −1.3 to −1.4 MPa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020112
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  • 5
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    ADAR RNA editing in human disease; more to it than meets the I (2017)
    Springer
    Details
    Publication Date: 2017-09-01
    Print ISSN: 0340-6717
    Electronic ISSN: 1432-1203
    Topics: Biology , Medicine
    Published by Springer
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