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  • Springer  (4)
  • 1980-1984  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Soldier-specific chemicals of the termiteCurvitermes strictinasus Mathews (Isoptera, Nasutitermitinae) (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 127-133 
    Details
    ISSN: 1573-1561
    Keywords: Isoptera ; Nasutitermitinae ; isolation ; identification ; Curvitermes strictinasus (Mathews) ; defense secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The defense secretion of the soldier termites ofCurvitermes strictinasus Mathews has been analyzed. Seven components have been isolated and identified, including limonene, terpinolene,p-cymen-8-ol, tridecan-2-one, tridecen-2-onecis,trans-farnesal, andtrans,trans-farnesal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00988640
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  • 2
    Electronic Resource
    Electronic Resource
    Chemical composition of the frontal gland secretion ofSyntermes soldiers (Isoptera, Termitidae) (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 135-145 
    Details
    ISSN: 1573-1561
    Keywords: Isoptera ; Nasutitermitinae ; Syntermes species ; defensive secretion ; isolation ; identification ; terpenoid compounds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The defensive secretions from the frontal glands of soldier termites of the genusSyntermes contain similar mixtures of mono- and sesquiterpene hydrocarbons. The major components inS. dirus, S. molestus, S. brevimalatus, S. peruanus, and a new species (Syntermes sp. n), iscis-β-ocimene. A substantial amount of aristolochene is found inSyntermes sp. n. and is present at lower levels in all the other species;S. brevimalatus contains onlycis-β-ocimene and aristolochene. The four other species also contain minor amounts of epi-α-selinene and germacrene A. The latter compound has been identified on the basis of its rearrangement product β-elemene. The termiteS. grandis differed markedly from the otherSyntermes species in that no terpenoid components were found in the soldier extract. With the obvious exception ofS. grandis, the same soldier-specific mono- and sesquiterpenes occurred in all species. The total amount of secretion per unit weight of soldiers varies with the species and is inversely proportional to the development of the mandibular apparatus. InS. molestus smaller gland size is compensated for by a greater number of soldiers foraging trails.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00988641
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of dominant hamster cell mutants resistant to oxygenated sterols (1982)
    Springer
    Somatic cell and molecular genetics 8 (1982), S. 557-574 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Stable mutants of Dede and CHO cells, resistant to suppression of cholesterogenesis by oxygenated sterols, have been isolated in a single step. Luria-Delbrück fluctuation analysis indicated a random occurrence of resistance at a rate of 1×10 −7 mutations/cell/generation. Cholesterol biosynthesis, 3-hydroxy-3-methylglutaryl coenzyme A reductase activity, and growth of the mutant cells were coordinately resistant to oxygenated sterols in the culture medium, and this resistance was expressed as a dominant traint in somatic cell hybrids of the wild-type and mutant cells. The dominant resistance was employed in the selection of various cell hybrids. There was complete additivity of reductase activities in mixed lysates of inhibited wild-type and uninhibited mutant cells, indicating that cytosolic (in)activation factors were not causative of this resistance. We suggest that oxygenated sterols are (co)repressors in suppression of the synthesis of the reductase and that the resistance mutant phenotypes result from altered regulatory loci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01542851
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  • 4
    Electronic Resource
    Electronic Resource
    Chromosome-mediated gene transfer of HPRT and APRT in an intraspecific human cell system (1983)
    Springer
    Somatic cell and molecular genetics 9 (1983), S. 175-188 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Chromosome-mediated transfer of genes between human cell lines was accomplished using HeLa cells as chromosome donors and HT1080 fibrosarcoma lines as recipients. This report describes the intraspecific transfer of two genetic markers, hypoxanthine-guanine-phosphoribosyl-transferase (HPRT +)and adenine phosphoribosyltransferase (APRT +).The isolation and characterization of the necessary enzyme-deficient (HPRT − and APRT −)recipient HT1080 cell lines are also described. The chromosome-mediated gene transfer was carried out using a modification of the procedure of Miller and Ruddle, including treatment of the donor chromosomes with calcium phosphate and subsequent exposure of the recipient cells of dimethyl sulfoxide. In experiments to optimize this procedure for HT1080 cell recipients, we found that a brief (2-min) exposure to high DMSO concentration (20%) was effective for enhancing transfer efficiencies in this system. Transfer frequencies (transferents per recipient cells assayed) averaged approximately 1×10−6 for HPRT + and were 〉2×10−6 for APRT +.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01543176
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