ALBERT

Description: Despite growing evidence that methane (CH4) formation could also occur in well-oxygenated surface fresh waters, its significance at the ecosystem scale is uncertain. Empirical models based on data gathered at high latitude predict that the contribution of oxic CH4 increases with lake size and should represent the majority of CH4 emissions in large lakes. However, such predictive models could not directly apply to tropical lakes, which differ from their temperate counterparts in some fundamental characteristics, such as year-round elevated water temperature. We conducted stable-isotope tracer experiments, which revealed that oxic CH4 production is closely related to phytoplankton metabolism and is a common feature in five contrasting African lakes. Nevertheless, methanotrophic activity in surface waters and CH4 emissions to the atmosphere were predominantly fuelled by CH4 generated in sediments and physically transported to the surface. Indeed, CH4 bubble dissolution flux and diffusive benthic CH4 flux were several orders of magnitude higher than CH4 production in surface waters. Microbial CH4 consumption dramatically decreased with increasing sunlight intensity, suggesting that the freshwater “CH4 paradox” might be also partly explained by photo-inhibition of CH4 oxidizers in the illuminated zone. Sunlight appeared as an overlooked but important factor determining the CH4 dynamics in surface waters, directly affecting its production by photoautotrophs and consumption by methanotrophs.

Description: We report a data-set obtained in the three branches (My Tho, Ham Luong, Co Chien) of the Mekong delta (Bến Tre province, Vietnam) in December 2003, April 2004, and October 2004, of biogeochemical variables related to carbon cycling (pH, total alkalinity (TA), O2 saturation level (%O2), calculated partial pressure of CO2 (pCO2), dissolved CH4 concentration, particulate (POC) and dissolved (DOC) organic carbon concentration and stable isotope composition (δ13C-POC, δ13C-DOC), particulate nitrogen (PN), dissolved inorganic carbon (DIC) stable isotope composition (δ13C-DIC), total suspended matter (TSM)). Both the inner estuary (upstream of the mouth) and the outer estuary (river plume) were sampled, as well as side channels. The values of pCO2 ranged between 232 and 4,085 ppm, %O2 between 63 and 114 %, and CH4 between 2 and 2,217 nmol L−1, within the ranges of values previously reported in temperate and tropical macro-tidal estuaries. Strong seasonal variations were observed. In the upper oligohaline estuary, low pCO2 (479–753 ppm) and high %O2 (98–106 %) values were observed in April 2004 most probably related to freshwater phytoplankton growth owing to low freshwater discharge (1,400 m3 s−1) and increase of water residence time; during the two other sampling periods with a higher freshwater discharge (9,300-17,900 m3 s−1), higher pCO2 (1,895–2,664 ppm) and lower %O2 (69–84 %) values were observed in the oligohaline part of the estuary. During the October 2004 sampling, important phytoplankton growth occurred in the off-shore part of the river plume as attested by changes in the contribution of POC to TSM (%POC), δ13C-POC, POC : PN ratios, possibly related to low TSM values (improvement of light conditions for phytoplankton development), leading to low pCO2 (232 ppm) and high %O2 (114 %) values. Water in the side channels in the Mekong delta was strongly impacted by inputs from the extensive shrimp farming ponds. The values of pCO2, CH4, %O2, δ13C-DIC indicated intense organic matter degradation that was partly mediated by sulfate reduction (presumably in sediments), as indicated by the slope of TA and DIC co-variations. The δ13C-POC variations also indicated intense phytoplankton growth in the side channels, presumably due to nutrient enrichment related to the shrimp farming ponds. A dataset in the mangrove creeks of the Ca Mau province (part of the Mekong delta) was also acquired in April 2004 and October 2004. These data extended the range of variability of pCO2 and %O2 with more extreme values than in the Mekong delta (Bến Tre), with maxima and minima of 6,912 ppm and 37 %, respectively. Similarly, the maximum CH4 concentration (686 nmol L−1) was higher in the Ca Mau province mangrove creeks than in the Mekong delta (Bến Tre, maximum 222 nmol L−1), during the October 2004 cruise (rainy season and high freshwater discharge period). In April 2004 (dry season and low freshwater discharge period), the CH4 values were much lower than in October 2004 (average 19 ± 13 and 210 ± 158 nmol L−1, respectively) in the Ca Mau province mangrove creeks, owing to the higher salinity (average 33.2 ± 0.6 and 14.1 ± 1.2, respectively) that probably led to higher sediment sulfate reduction, leading to inhibition of sediment methanogenesis and higher anaerobic CH4 oxidation. In the inner estuarine region (three branches of the Mekong delta), CO2 emissions to the atmosphere averaged 121 mmol m−2 d−1, and the CH4 emissions averaged 118 µmol m−2 d−1. The CO2 emission to the atmosphere from the Mekong inner estuary was higher than reported in the Yangtze and Pearl River inner estuaries. This was probably due to the lower salinity in the Mekong delta branches, possibly due to different morphology; relatively linear channels in the Mekong delta versus funnel-shaped estuaries for the Yangtze and Pearl River inner estuaries.

Description: Quantification of sediment and carbon (C) fluxes in rivers with strong seasonal and inter-annual variability presents a challenge for global flux estimates as measurement periods are often too short to cover all hydrological conditions. We studied the dynamics of the Tana River (Kenya) from 2012 to 2014 through daily monitoring of sediment concentrations at three sites (Garissa, Tana River Primate Reserve and Garsen) and daily monitoring of C concentrations in Garissa and Garsen during three distinct seasons. In wet seasons, C fluxes were dominated by particulate organic C (POC) and decreased downstream. Dry season fluxes of dissolved inorganic C (DIC) and POC had a similar share in total C flux at both locations while POC fluxes increased downstream. The dissolved organic C (DOC) flux did not show strong spatial nor temporal variations. The construction of constituent rating curves with a bootstrap method in combination with daily discharge data (1942–2014) provided potential sediment and C flux ranges as a function of annual discharge. At low annual discharge, our estimates generally predict a net decrease of sediment and C storage between the upstream and downstream site. As the annual discharge increases, our simulations shift toward net retention. This analysis allowed us to infer how variations in discharge regime, related to climate or human impacts, may affect riverine fluxes. Overall, we estimate that retention was dominant: integration over all simulations resulted in an average net retention of sediment (~2.9 Mt yr−1), POC (~18000 tC yr−1), DOC (~920 tC yr−1) and DIC (~1200 tC yr−1) over the 73 years of discharge measurements.

Description: Inland waters impart considerable influence on nutrient cycling and budget estimates across local, regional and global scales, whilst anthropogenic pressures, such as rising populations and the appropriation of land and water resources, are undoubtedly modulating the flux of carbon (C), nitrogen (N), and phosphorus (P) between terrestrial biomes to inland waters, and the subsequent flux of these nutrients to the marine and atmospheric domains. Here, we present a two year biogeochemical record (October 2011–December 2013) at bi-weekly sampling resolution for the lower Sabaki River, Kenya, and provide estimates for suspended sediment and nutrient export fluxes from the Athi-Galana-Sabaki (A-G-S) river basin under pre-dam conditions, and in light of the approved construction of the Thwake Multi-purpose Dam on the Athi River. Erratic seasonal variation was typical for most parameters, with generally poor correlation between discharge and material concentrations and stable isotopic signatures of C (δ13C) and N (δ15N). Although high total suspended matter (TSM) concentrations are reported here (up to ~ 3.8 g L−1), peak concentrations of TSM rarely coincided with peak discharge. The contribution of particulate organic C (POC) to the TSM pool indicates a wide bi-annual variation in suspended sediment load from OC-poor (0.3 %) to OC-rich (14.9 %), with the highest %POC occurring when discharge is  80 % of the total load for TSM (~ 86 %), POC (~ 89 %), DOC (~ 81 %), PN (~ 89 %) and TPP (~ 82 %), with 〉 50 % of each fraction exported during the long wet season (March–May). Our estimated sediment yield of 85 Mg km−2 yr−1 is relatively low on the global scale and is considerably less than the recently reported average sediment yield of ~ 630 Mg km−2 yr−1 for African river basins. Regardless, sediment and OC yields were all at least equivalent or greater than reported yields for the neighbouring and flow-regulated Tana River. Rapid pulses of heavily 13C-enriched POC coincided with peak concentrations of PN, ammonium, CH4 and low dissolved oxygen saturation, lead to the suggestion that large mammalian herbivores (e.g. hippopotami) may mediate the delivery of C4 organic matter to the river during the dry season. Given recent projections for increasing dissolved nutrient export from African rivers, as well as planned flow regulation on the Athi River, these first estimates of material fluxes from the Sabaki River provide base-line data for future research initiatives assessing anthropogenic perturbation of the A-G-S river basin.

Description: We carried out 10 field expeditions between 2010 and 2015 in the lowland part of the Congo River network in the eastern part of the basin (Democratic Republic of the Congo), to describe the spatial variations in fluvial dissolved carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) concentrations. We investigate the possible drivers of the spatial variations in dissolved CO2, CH4 and N2O concentrations by analyzing covariations with several other biogeochemical variables, aquatic metabolic processes (primary production and respiration), catchment characteristics (land cover) and wetland spatial distributions. We test the hypothesis that spatial patterns of CO2, CH4 and N2O are partly due to the connectivity with wetlands, in particular with a giant wetland of flooded forest in the core of the Congo basin, the “Cuvette Centrale Congolaise” (CCC). Two transects of 1650 km were carried out from the city of Kisangani to the city of Kinshasa, along the longest possible navigable section of the river and corresponding to 41 % of the total length of the main stem. Additionally, three time series of CH4 and N2O were obtained at fixed points in the main stem of the middle Congo (2013–2018, biweekly sampling), in the main stem of the lower Kasaï (2015–2017, monthly sampling) and in the main stem of the middle Oubangui (2010–2012, biweekly sampling). The variations in dissolved N2O concentrations were modest, with values oscillating around the concentration corresponding to saturation with the atmosphere, with N2O saturation level (%N2O, where atmospheric equilibrium corresponds to 100 %) ranging between 0 % and 561 % (average 142 %). The relatively narrow range of %N2O variations was consistent with low NH4+ (2.3±1.3 µmol L−1) and NO3- (5.6±5.1 µmol L−1) levels in these near pristine rivers and streams, with low agriculture pressure on the catchment (croplands correspond to 0.1 % of catchment land cover of sampled rivers), dominated by forests (∼70 % of land cover). The covariations in %N2O, NH4+, NO3- and dissolved oxygen saturation level (%O2) indicate N2O removal by soil or sedimentary denitrification in low O2, high NH4+ and low NO3- environments (typically small and organic matter rich streams) and N2O production by nitrification in high O2, low NH4+ and high NO3- (typical of larger rivers that are poor in organic matter). Surface waters were very strongly oversaturated in CO2 and CH4 with respect to atmospheric equilibrium, with values of the partial pressure of CO2 (pCO2) ranging between 1087 and 22 899 ppm (equilibrium ∼400 ppm) and dissolved CH4 concentrations ranging between 22 and 71 428 nmol L−1 (equilibrium ∼2 nmol L−1). Spatial variations were overwhelmingly more important than seasonal variations for pCO2, CH4 and %N2O as well as day–night variations for pCO2. The wide range of pCO2 and CH4 variations was consistent with the equally wide range of %O2 (0.3 %–122.8 %) and of dissolved organic carbon (DOC) (1.8–67.8 mg L−1), indicative of generation of these two greenhouse gases from intense processing of organic matter either in “terra firme” soils, wetlands or in-stream. However, the emission rate of CO2 to the atmosphere from riverine surface waters was on average about 10 times higher than the flux of CO2 produced by aquatic net heterotrophy (as evaluated from measurements of pelagic respiration and primary production). This indicates that the CO2 emissions from the river network were sustained by lateral inputs of CO2 (either from terra firme or from wetlands). The pCO2 and CH4 values decreased and %O2 increased with increasing Strahler order, showing that stream size explains part of the spatial variability of these quantities. In addition, several lines of evidence indicate that lateral inputs of carbon from wetlands (flooded forest and aquatic macrophytes) were of paramount importance in sustaining high CO2 and CH4 concentrations in the Congo river network, as well as driving spatial variations: the rivers draining the CCC were characterized by significantly higher pCO2 and CH4 and significantly lower %O2 and %N2O values than those not draining the CCC; pCO2 and %O2 values were correlated to the coverage of flooded forest on the catchment. The flux of greenhouse gases (GHGs) between rivers and the atmosphere averaged 2469 mmol m−2 d−1 for CO2 (range 86 and 7110 mmol m−2 d−1), 12 553 µmol m−2 d−1 for CH4 (range 65 and 597 260 µmol m−2 d−1) and 22 µmol m−2 d−1 for N2O (range −52 and 319 µmol m−2 d−1). The estimate of integrated CO2 emission from the Congo River network (251±46 TgC (1012 gC) yr−1), corresponding to nearly half the CO2 emissions from tropical oceans globally (565 TgC yr−1) and was nearly 2 times the CO2 emissions from the tropical Atlantic Ocean (137 TgC yr−1). Moreover, the integrated CO2 emission from the Congo River network is more than 3 times higher than the estimate of terrestrial net ecosystem exchange (NEE) on the whole catchment (77 TgC yr−1). This shows that it is unlikely that the CO2 emissions from the river network were sustained by the hydrological carbon export from terra firme soils (typically very small compared to terrestrial NEE) but most likely, to a large extent, they were sustained by wetlands (with a much higher hydrological connectivity with rivers and streams).

Description: Large rivers transport considerable amounts of terrestrial dissolved organic matter (DOM) to the ocean. Yet, downstream gradients and temporal variability in DOM fluxes and characteristics are poorly studied at the scale of large river basins, especially in tropical areas. Here, we report longitudinal patterns in DOM content and composition based on absorbanc e and fluorescence measurements along the Zambezi River and its main tributary, the Kafue River, during two hydrological seasons. During high flow periods, a greater proportion of aromatic and humic DOM was mobilized along rivers due to the hydrological connectivity with wetlands and high flow velocities, while low flow periods were characterized by lower DOM content of less aromaticity resulting from loss of connectivity with wetlands, more efficient degradation of terrestrial DOM and enhanced autochthonous productivity. Changes in water residence time due to contrasting water discharge were found to modulate the fate of DOM along the river continuum. Thus, terrestrial DOM dynamics shifted from transport-dominated during the wet seasons towards degradation during the dry season, with substantial consequences on longitudinal DOM content and composition. The longitudinal evolution of DOM was also strongly impacted by a hydrological buffering effect in large reservoirs in which the seasonal variability of DOM fluxes and composition was strongly reduced.

Description: Marine sediments, particularly those located in estuarine and coastal zones, are key locations for the burial of organic carbon (C). However, organic C delivered to the sediment is subjected to a range of biological C-cycling processes, the rates and relative importance of which vary markedly between sites, and which are thus difficult to predict. In this study, stable isotope tracer experiments were used to quantify the processing of C by microbial and faunal communities in two contrasting Scottish estuarine sites: a subtidal, organic C rich site in Loch Etive with cohesive fine-grained sediment, and an intertidal, organic C poor site on an Ythan estuary sand flat with coarse-grained permeable sediments. In both experiments, sediment cores were recovered and amended with 13C labelled phytodetritus to quantify whole community respiration of the added C and to trace the isotope label into faunal and bacterial biomass. Similar respiration rates were found in Loch Etive and on the Ythan sand flat (0.64 ± 0.04 and 0.63 ± 0.12 mg C m−2h−1, respectively), which we attribute to the experiments being conducted at the same temperature. Faunal uptake of added C over the whole experiment was markedly greater in Loch Etive (204 ± 72 mg C m−2) than on the Ythan sand flat (0.96 ± 0.3 mg C m−2), and this difference was driven by a difference in both faunal biomass and activity. Conversely, bacterial C uptake over the whole experiment in Loch Etive was much lower than that on the Ythan sand flat (1.80 ± 1.66 and 127 ± 89 mg C m−2, respectively). This was not driven by differences in biomass, indicating that the bacterial community in the permeable Ythan sediments was particularly active, being responsible for 48 ± 18 % of total biologically processed C. This type of biological C processing appears to be favoured in permeable sediments. The total amount of biologically processed C was greatest in Loch Etive, largely due to greater faunal C uptake, which was in turn a result of higher faunal biomass. When comparing results from this study with a wide range of previously published isotope tracing experiments, we found a strong correlation between total benthic biomass (fauna plus bacteria) and total biological C processing rates. Therefore, we suggest that the total C-cycling capacity of benthic environments is primarily determined by total biomass.

Description: Large rivers transport considerable amounts of terrestrial dissolved organic matter (DOM) to the ocean. However, downstream gradients and temporal variability in DOM fluxes and characteristics are poorly studied at the scale of large river basins, especially in tropical areas. Here, we report longitudinal patterns in DOM content and composition based on absorbance and fluorescence measurements along the Zambezi River and its main tributary, the Kafue River, during two hydrological seasons. During high-flow periods, a greater proportion of aromatic and humic DOM was mobilized along rivers due to the hydrological connectivity with wetlands, while low-flow periods were characterized by lower DOM content of less aromaticity resulting from loss of connectivity with wetlands, more efficient degradation of terrestrial DOM and enhanced autochthonous productivity. Changes in water residence time due to contrasting water discharge were found to modulate the fate of DOM along the river continuum. Thus, high water discharge promotes the transport of terrestrial DOM downstream relative to its degradation, while low water discharge enhances the degradation of DOM during its transport. The longitudinal evolution of DOM was also strongly impacted by a hydrological buffering effect in large reservoirs in which the seasonal variability of DOM fluxes and composition was strongly reduced.

Description: The role of river ecosystems in processing inputs of dissolved organic matter (DOM) from the terrestrial environment during downstream transport in river networks is poorly constrained. In this study we report a data-set of DOM concentrations (dissolved organic carbon) and composition (stable carbon isotopic composition, absorption and fluorescence properties) acquired along a 1700 km stretch in the Congo River Basin, the second river in the World. Samples were collected in the main river and its tributaries in the central part of the Basin during high waters (HW) and falling waters (FW) periods. The longitudinal increase in DOC concentrations and changes in DOM characteristics along the mainstem was found to differ between the two periods, especially because of greater photodegradation of terrestrial inputs from the DOM-rich waters from the Cuvette Centrale during FW as water residence time (WRT) increased. DOM degradation within the Congo Basin was found to result in the transition from aromatic to aliphatic DOM, resulting from the losses of aromatic compounds by photodegradation and the production of aliphatic compound by biological degradation. This study highlights that landscape properties and changes in WRT can play a major role on the functioning of river ecosystems in processing DOM during its downstream transformation in river networks.

Description: The processing of terrestrially derived dissolved organic matter (DOM) during downstream transport in fluvial networks is poorly understood. Here, we report a dataset of dissolved organic carbon (DOC) concentrations and DOM composition (stable carbon isotope ratios, absorption and fluorescence properties) acquired along a 1700 km transect in the middle reach of the Congo River basin. Samples were collected in the mainstem and its tributaries during high-water (HW) and falling-water (FW) periods. DOC concentrations and DOM composition along the mainstem were found to differ between the two periods because of a reduced lateral mixing between the central water masses of the Congo River and DOM-rich waters from tributaries and also likely because of a greater photodegradation during FW as water residence time (WRT) increased. Although the Cuvette Centrale wetland (one of the world's largest flooded forests) continuously releases highly aromatic DOM in streams and rivers of the Congo Basin, the downstream transport of DOM was found to result in an along-stream gradient from aromatic to aliphatic compounds. The characterization of DOM through parallel factor analysis (PARAFAC) suggests that this transition results from (1) the losses of aromatic compounds by photodegradation and (2) the production of aliphatic compounds by biological reworking of terrestrial DOM. Finally, this study highlights the critical importance of the river–floodplain connectivity in tropical rivers in controlling DOM biogeochemistry at a large spatial scale and suggests that the degree of DOM processing during downstream transport is a function of landscape characteristics and WRT.