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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 47 (1979), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Protoplasts were isolated from leaves of in vitro grown plants of Nicotiana rustica L. by the one step enzymatic method. With 3% cetlulase in 0.5 M mannitol at 25°C and pH 4.6, within 10–12 h about half the total cells were transformed into protoplasts. The enzyme activity had two pH maxima, one at pH 3.5 and the other at pH 6.5, indicating the presence of isoenzymes. A time-course study at different temperatures indicated that ai 30°C the protoplasts’ liberation was quicker but it resulted in their subsequent bursting. The protoplasts were cultured in liquid as well as on agar-jelled medium of Ohyama and Nitsch (1972) supplemented with 2.4-D and benzylaminopurine, each at I mg/1. and 14% sucrose. Regular divisions could be seen only on agar-jelted medium. Colonies on transfer to Murashige and Skoog (1962) medium, containing IAA and kinetin, differentiated into plantlets. These plants, within 2 months, flowered in vitro and set seed of which about 20% germinated.
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Ground water 13 (1975), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: Ground water of Shiraz alluvial basin in Zagros range (south Iran) shows three principal anion types, namely chloride, sulfate and bicarbonate. Intermediate types between these groups are also present, which seem to represent modifications in ground water due to diagenesis.Possible chemical origin of the ground water is mainly due to two major processes either acting individually or collectively, i.e. solution of minerals of the geological formation and mixing of ground water accompanied by the precipitation of some constituents such as calcium.
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 260 (1976), S. 508-509 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Hill6 used a self-gravitating galactic disk model of the galactic gas distribution that was exposed to an ionising photon flux calculated on a variety of possible extrapolations of the high latitude soft X-ray data to lower energies. Bochkarev and Sunyaev7 used a variety of possible gas density ...
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 45 (1976), S. 411-420 
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of flooding on chemical composition of solutions of two alkaline calcareous soils were studied for incubation periods of 0, 2, 3, 4, 5 and 8 weeks. Flooding slightly reduced pH of both the soils. The concentration of Ca, Mg, HCO3, Mn, Fe, P, and total soluble salts markedly increased. This occurred rapidly in the beginning but at a much slower rate later on during incubation. Drying the soils from 7th to 8th week of submergence greatly depressed their concentration. Flooding had no effect on Cu but increased Zn concentration. The contents of various ions in submerged soils generally had little relationship to those in dry soils. Manganese and Fe strongly depressed 65 Zn absorption by rice (Oryza sativa L.) from solution cultures. Flooding induced increased contents of various ions in soil solutions appear to be a major cause of accentuating Zn and Cu deficiency in rice and of obscuring the predictability of soil tests for Zn and Cu availability to lowland rice. re]19750515
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 47 (1977), S. 297-302 
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary By contrast of strong phosphorus-zinc antagonism in upland crops, P strongly enhanced Zn uptake in flooded rice on a calcareous soil. Radioisotopic studies indicated increase to occur preferentially from applied Zn fertilizer. Phosphorus appeared to stimulate uptake predominantly by enhancing Zn concentration in soil solution and by increasing metabolic Zn absorption by plant roots. The ‘A’ value was not a reliable measure of labile soil Zn for rice as it markedly changed with levels of P and Zn fertilizers.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 45 (1976), S. 613-623 
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Short-term Zn absorption by rice (Oryza sativa L.) increased gradually with increasing concentration of solution Zn from 0.08 to 10 μM. Two fine-grained rice varieties absorbed Zn by two mechanisms; one operated at lower, relevant to deficient and marginal and the other at higher, relevant to optimum and toxic soil Zn concentrations. Coarse-grained rice variety absorbed Zn only by one mechanism. Hydrogen strongly depressed Zn absorption by rice within pH range of 7.2 to 5.5. From pH 5.5 to 4.5, it was severely toxic to Zn absorption mechanism. Toxic effect is not important for submerged rice where soil pH is usually 7.0. Increasing Ca concentration from 500 μM to 5 mM depressed Zn absorption by rice from Zn concentrations of both the mechanism. The effect was higher for the first mechanism of low solution Zn contents. Magnesium and Cu inhibited Zn absorption by rice non-competitively from the first and competitively from the second mechanism. Calcium, Mg, Fe, Mn, and P depressed Zn absorption by rice also from soil percolates. Increased contents of inhibiting ions with soil submergence is responsible for higher incidence of Zn deficiency in lowland rice than in wheat and other upland crops. They seem to be also responsible for obscuring the predictability of soil Zn tests for lowland rice. The present studies fail to explain higher incidence of Cu-Zn antagonism in wheat than in rice. re]19750515
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 46 (1977), S. 649-654 
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Nitrogen application increased Zn contents of flooded rice on two calcareous soils. Urea and (NH4)2SO4 being better N carriers than NH4NO3 resulted in higher increase. Nitrogen enhanced Zn contents partly through growth promotion but mainly by increasing soil Zn solubility and root efficiency for Zn absorption. Zinc solubility rose by an enigmatic mechanism and not from pH reduction or soluble Zn-HN3 complex formation as occurs for upland plants. Nitrogen aggravated Zn retention in upland plant roots as immobile Zn-protein complex was not important for rice. Bicarbonate inhibition of Zn uptake by rice from CO(NH2)2 application or its stimulation by lower redox potential from NH4NO3 addition were not involved.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 45 (1976), S. 397-410 
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary On several alkaline calcareous soils, Zn and Cu deficiency occurred mainly in lowland rice (Oryza sativa L.) and was rarely found in wheat (Triticum aestivum L.). Zinc and Cu requirement of plants was not responsible as the critical Zn and Cu contents in tops of the two plant species were almost similar i.e. 17.4, 6.5 and 14.5, 5.6 ppm respectively. Neither did rice absorb Zn and Cu less efficiently. On the contrary, their rates of absorption in rice were double than in wheat. They were 22.2, 6.3 and 10.2, 3.3 ng atoms/g fresh root/h respectively in the two plant species. Flooded soil conditions appeared to be responsible for Zn and Cu deficiency in rice as their deficiency was found mainly in plant samples collected from continuously flooded fields. The mechanism is not known. Both Zn and Cu inhibited uptake of each other in wheat on most of the soils. In rice, only applied Zn depressed Cu uptake but Cu had generally little effect on Zn uptake. Little Cu inhibition of Zn uptake in lowland rice seems to be related to flooded soil conditions. The mechanism is yet to be known. The antagonising element accentuated the deficiency of the other element both in wheat and rice and severely reduced their yields on soils marginal to deficient in Zn or Cu supplies. It is recommended that their soil availability status should be thoroughly considered before their fertilizers are applied. re]19750515
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 46 (1977), S. 637-642 
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The mechanism of differential susceptibility of two rice (Oryza sativa L.) varieties to Zn deficiency was explored in 20 field trials. IR-6 rice suffered with Zn deficiency much more severely than Basmati-370. Higher Fe absorption seems to be mainly responsible. It achieved this effect by strongly inhibiting Zn absorption and by markedly increasing internal Zn requirement as IR-6 plants.
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  • 10
    ISSN: 1615-6102
    Keywords: Cold treatment ; Datura innoxia ; Haploids ; Pollen embryos
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary This study concerns the development of pollen embryos as affected by various physical conditions of culture in media devoid of hormones. Freshly isolated pollen, from anthers ofDatura, failed to form embryos regardless of whether they were cultured on liquid or solid medium. In contrast, pollen isolated from anthers precultured on solid medium did form embryos and the response could be increased by prior cold treatment of anthers at 4 °C for 4 days. However, the best results were obtained when anthers were cultured from the very beginning in liquid medium and transferred serially to fresh medium. Under such conditions, the anthers dehisced, allowing spontaneous shedding of pollen grains. It was thus possible to have several fractions of shed pollen continuing their development into embryos. When serial culture was started with anthers from cold-treated buds not only were embryos formed in all the fractions of shed pollen but the frequency was also considerably higher than in any mode of culturing.
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