Publication Date:
1994-12-02
Description:
Exocytosis in mammalian spermatozoa (the acrosome reaction) is a process essential for fertilization. Both progesterone and zona pellucida induce exocytosis in spermatozoa, which may encounter both during penetration of the oocyte's vestments. When mouse spermatozoa were exposed first to progesterone and then to zona pellucida, exocytosis was enhanced to a greater degree than that seen when the agonists were presented together or in the inverse order, which suggests that the steroid exerts a priming effect. Progesterone similarly primed the generation of intracellular messengers evoked by zona pellucida. The effects triggered by progesterone were mimicked by gamma-aminobutyric acid (GABA) and were blocked by bicuculline, which indicates that the steroid acts on a GABAA receptor.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Roldan, E R -- Murase, T -- Shi, Q X -- New York, N.Y. -- Science. 1994 Dec 2;266(5190):1578-81.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Development and Signalling, Babraham Institute, Biotechnology and Biological Sciences Research Council, Cambridge, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7985030" target="_blank"〉PubMed〈/a〉
Keywords:
Acrosome/drug effects/*physiology
;
Animals
;
Bicuculline/pharmacology
;
Calcium/metabolism
;
Diglycerides/metabolism
;
*Exocytosis/drug effects
;
Male
;
Mice
;
Phosphatidylinositol 4,5-Diphosphate
;
Phosphatidylinositol Phosphates/metabolism
;
Phospholipase D/metabolism
;
Progesterone/*pharmacology
;
Receptors, GABA/physiology
;
Spermatozoa/drug effects/*physiology
;
Zona Pellucida/*physiology
;
gamma-Aminobutyric Acid/pharmacology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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