ISSN:
1432-072X
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Summary 1. Normal cells of the Nichols non-pathogenic strain of Treponema pallidum were disrupted by one or a combination of physical and chemical procedures. Cells and cellular fragments were then observed in the electron microscope by means of three techniques: negative staining, thin sectioning, and freeze-etching. Changes in typical morphology were noted and individual organelles were isolated for detailed structural analysis. 2. The cell envelope, a triple-layered structure, was sensitive to trypsin, sodium deoxycholate, sodium lauryl sulfate, diethyl ether, hyaluronidase and a combination of lysozyme and rapid freeze-thawing. The envelope contained polygonal subunits that consisted of at least three components. 3. The cover of the protoplasmic cylinder was composed of two unit membranes interpreted to be the cell wall and cell membrane. The cell wall, which retained its spiral shape after removal of the cell envelope, was sensitive to lysozyme. The cell membrane was the site of attachment of the axial filaments. 4. A band of parallel fibrils, attached to the innermost layer of the cell membrane, wrapped around the protoplasmic cylinder in a spiral fashion. The fibrils, sensitive to trypsin, were released from the cell membrane by sodium lauryl sulfate
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00408528
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