Publication Date:
2018-07-24
Description:
RESEARCH
Open Access
An atlas of larval organogenesis in the
European shore crab
Carcinus maenas
L.
(Decapoda, Brachyura, Portunidae)
Franziska Spitzner
1,2
, Rebecca Meth
1,2
, Christina Krüger
1
, Emanuel Nischik
1
, Stefan Eiler
1,3
, Andy Sombke
1
,
Gabriela Torres
2
†
and Steffen Harzsch
1*
†
Abstract
Background:
The life history stages of brachyuran crustaceans include pelagic larvae of the Zoea type which grow
by a series of moults from one instar to the next. Zoeae actively feed and possess a wide range of organ systems
necessary for autonomously developing in the plankton. They also display a rich behavioural repertoire that allows
for responses to variations in environmental key factors such as light, hydrostatic pressure, tidal currents, and
temperature. Brachyuran larvae have served as distinguished models in the field of Ecological Developmental
Biology fostering our understanding of diverse ecophysiological aspects such as phenotypic plasticity, carry-over
effects on life-history traits, and adaptive mechanisms that enhance tolerance to fluctuations in environmental
abiotic factors. In order to link such studies to the level of tissues and organs, this report analyses the internal
anatomy of laboratory-reared larvae of the European shore crab
Carcinus maenas
. This species has a native
distribution extending across most European waters and has attracted attention because it has invaded five
temperate geographic regions outside of its native range and therefore can serve as a model to analyse thermal
tolerance of species affected by rising sea temperatures as an effect of climate change.
Results:
Here, we used X-ray micro-computed tomography combined with 3D reconstruction to describe
organogenesis in brachyuran larvae. We provide a detailed atlas of the larval internal organization to complement
existing descriptions of its external morphology. In a multimethodological approach, we also used cuticular
autofluorescence and classical histology to analyse the anatomy of selected organ systems.
Conclusions:
Much of our fascination for the anatomy of brachyuran larvae stems from the opportunity to observe
a complex organism on a single microscopic slide and the realization that the entire decapod crustacean bauplan
unfolds from organ anlagen compressed into a miniature organism in the sub-millimetre range. The combination
of imaging techniques used in the present study provides novel insights into the bewildering diversity of organ
systems that brachyuran larvae possess. Our analysis may serve as a basis for future studies bridging the fields of
evolutionary developmental biology and ecological developmental biology
Repository Name:
EPIC Alfred Wegener Institut
Type:
Article
,
isiRev
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