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  • 1
    Publication Date: 2024-01-12
    Description: The genus Gielisella gen. n., belonging to the Elachistidae: Parametriotinae is described with two new species from southern Spain: its type species G. clarkeorum sp. n. and G. nigripalpis sp. n., both only known from adults collected at light. DNA barcodes of both species are provided. The taxonomic history of the Parametriotinae is discussed and a catalogue of the 35 recognised genera is provided as appendix. The arguments for erecting a new genus are discussed and we hypothesize that this constitutes an overlooked native Palaearctic element, although we cannot completely rule out the possibility of imported exotic species.
    Keywords: new species ; new genus ; Southern Spain ; Elachistidae
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
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  • 2
    Publication Date: 2024-01-12
    Description: Stigmella tatrica sp. n. is described from moths taken in the alpine zone of the Tatra Mountains in Slovakia. The new species is similar to several other montane species of Stigmella Schrank, 1802 in the S. aurella (Fabricius, 1775) group in external characters and male genitalia; its closest relative is S. dryadella (O. Hofmann, 1868). It is indistinguishable from S. tormentillella (Herrich-Sch\xc3\xa4ffer, 1860) by the colour and pattern elements of the forewing; however, it differs in the male genitalia in the shape and number of cornuti, in the female genitalia by long apophyses with protruding ovipositor, and by COI barcodes. Immature stages are unknown, but Dryas octopetala L. (Rosaceae) is the possible hostplant. The long ovipositor suggests an unusual, possibly hidden place for oviposition. The male and female adults and genitalia of both sexes are figured and photographs of the habitat are provided.
    Keywords: leaf miners ; moths ; Slovakia ; new species
    Repository Name: National Museum of Natural History, Netherlands
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  • 3
    Publication Date: 2024-01-12
    Description: Trois m\xc3\xa2les de Catapterix crimaea Zagulajev & Sinev, 1988, ont \xc3\xa9t\xc3\xa9 d\xc3\xa9couverts en France m\xc3\xa9ridionale, esp\xc3\xa8ce m\xc3\xa9connue seulement cit\xc3\xa9e de Crim\xc3\xa9e, appartenant \xc3\xa0 la petite famille des Acanthopteroctetidae (famille nouvelle pour la France) dans la superfamille des Neopseustoidea (superfamille nouvelle pour la France). Catapterix crimaea est la seuleesp\xc3\xa8ce pal\xc3\xa9arctique connue des Acanthopteroctetidae. \nThree males of Catapterix crimaea Zagulajev & Sinev, 1988, were discovered in southern France. This little known species, belonging to the family Acanthopteroctetidae (new family for France), superfamily Neopseustoidea (new superfamily for France) was previously only recorded from the Crimea. Catapterix crimaea is the only known Palearctic species of the Acanthopteroctetidae. The small moths (5,5-6,5 mm wingspan) with their golden scales, head with erect hairscales, short tongue, long mandibular palps resemble some Nepticulidae, Heliozelidae or icropterigidae. Other characters include a single spur on the mid tibia (as in Eriocraniidae), and a single frenular bristle. The female and biology are unknown.
    Keywords: Acanthopteroctetidae ; France ; new species ; small moths
    Repository Name: National Museum of Natural History, Netherlands
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  • 4
    Publication Date: 2024-01-12
    Description: Additions and corrections to the lists of Lepidoptera of Tula Province are given, comprising 33 species, 23 of which are listed for the province for the first time, incl. 6 species (Stigmella obliquella (Heinemann, 1862), Phyllonorycter nicellii (Stainton, 1851), Insalebria serraticornella (Zeller, 1839), Scopula subpunctaria (Herrich-Sch\xc3\xa4ffer, 1847), Apamea epomidion (Haworth, 1809), Agrochola macilenta (H\xc3\xbcbner, [1809])) \xe2\x80\x93 for the first time for Central European Russia. Also provided are northernmost or nort-westernmost records in European Russia for Cydia pyrivora (Danilevsky, 1947), Eupithecia addictata Dietze, 1908, Eublemma rosea (H\xc3\xbcbner, 1790), Trichoplusia ni (H\xc3\xbcbner, [1803]), Cryphia fraudatricula (H\xc3\xbcbner, [1803]), Athetis lepigone (M\xc3\xb6schler, 1860) \xd0\xb8 Mythimna l-album (Linnaeus, 1767).
    Keywords: lepidoptera ; Tula province ; additions and corrections
    Repository Name: National Museum of Natural History, Netherlands
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  • 5
    Publication Date: 2024-01-12
    Description: Heliozelidae are a widespread, evolutionarily early diverging family of small, day-flying monotrysian moths, for which a comprehensive phylogeny is lacking. We generated the first molecular phylogeny of the family using DNA sequences of two mitochondrial genes (COI and COII) and two nuclear genes (H3 and 28S) from 130 Heliozelidae specimens, including eight of the twelve known genera: Antispila, Antispilina, Coptodisca, Heliozela, Holocacista, Hoplophanes, Pseliastis, and Tyriozela. Our results provide strong support for five major Heliozelidae clades: (i) a large widespread clade containing the leaf-mining genera Antispilina, Coptodisca and Holocacista and some species of Antispila, (ii) a clade containing most of the described Antispila, (iii) a clade containing the leafmining genus Heliozela and the monotypic genus Tyriozela, (iv) an Australian clade containing Pseliastis and (v) an Australian clade containing Hoplophanes. Each clade includes several new species and potentially new genera. Collectively, our data uncover a rich and undescribed diversity that appears to be especially prevalent in Australia. Our work highlights the need for a major taxonomic revision of the family and for generating a robust molecular phylogeny using multi-gene approaches in order to resolve the relationships among clades.
    Keywords: Lepidoptera ; Multilocus phylogeny ; taxonomy ; family-level phylogeny ; Australia
    Repository Name: National Museum of Natural History, Netherlands
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  • 6
    Publication Date: 2024-01-12
    Description: We report the first identification of a sex pheromone in a heliozelid moth, Holocacista capensis van Nieukerken & Geertsema. This leafminer recently infested grapevine in South Africa. Compared to solvent extraction of pheromone glands, solid phase microextraction (SPME) proved to be highly effective for collection of the pheromone from calling females. The volatiles collected by SPME were analyzed by gas chromatography with electroantennographic detection (GC/EAD). Three compounds eliciting electrophysiological activity from the male antenna were identified as (Z)-5-tetradecenal, (Z)-7-tetradecenal, and (Z)-9-hexadecenal by coupled gas chromatography\xe2\x80\x93mass spectrometry (GC/MS). GC/MS analysis of dimethyldisulphide (DMDS) derivatives of fatty acyl moieties in pheromone gland extracts confirmed the presence of the corresponding putative pheromone precursors with double bonds in the same position and with Z geometry. Field trapping experiments in a South African vineyard confirmed that both (Z)-5-tetradecenal and (Z)-7-tetradecenal are essential for the attraction of male H. capensis, whereas addition of (Z)-9-hexadecenal to the blend did not affect the attractiveness. The composition of the pheromone is discussed in relation to the phylogeny of this family of moths.
    Keywords: Sex pheromone ; leafminer ; Heliozelidae ; Holocacista capensis ; primitive moth ; vineyard pest ; Vitis vinifera ; (Z)-5-tetradecenal ; (Z)-7-tetradecenal ; Lepidoptera
    Repository Name: National Museum of Natural History, Netherlands
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  • 7
    Publication Date: 2024-01-12
    Description: The proliferation of DNA data is revolutionizing all fields of systematic research. DNA barcode sequences, now available for millions of specimens and several hundred thousand species, are increasingly used in algorithmic species delimitations. This is complicated by occasional incongruences between species and gene genealogies, as indicated by situations where conspecific individuals do not form a monophyletic cluster in a gene tree. In two previous reviews, non-monophyly has been reported as being common in mitochondrial DNA gene trees. We developed a novel web service \xe2\x80\x9cMonophylizer\xe2\x80\x9d to detect non-monophyly in phylogenetic trees and used it to ascertain the incidence of species non-monophyly in COI (a.k.a. cox1) barcode sequence data from 4977 species and 41,583 specimens of European Lepidoptera, the largest data set of DNA barcodes analyzed from this regard. Particular attention was paid to accurate species identification to ensure data integrity. We investigated the effects of tree-building method, sampling effort, and other methodological issues, all of which can influence estimates of non-monophyly. We found a 12% incidence of non-monophyly, a value significantly lower than that observed in previous studies. Neighbor joining (NJ) and maximum likelihood (ML) methods yielded almost equal numbers of non-monophyletic species, but 24.1% of these cases of non-monophyly were only found by one of these methods. Non-monophyletic species tend to show either low genetic distances to their nearest neighbors or exceptionally high levels of intraspecific variability. Cases of polyphyly in COI trees arising as a result of deep intraspecific divergence are negligible, as the detected cases reflected misidentifications or methodological errors. Taking into consideration variation in sampling effort, we estimate that the true incidence of non-monophyly is ~23%, but with operational factors still being included. Within the operational factors, we separately assessed the frequency of taxonomic limitations (presence of overlooked cryptic and oversplit species) and identification uncertainties. We observed that operational factors are potentially present in more than half (58.6%) of the detected cases of non-monophyly. Furthermore, we observed that in about 20% of non-monophyletic species and entangled species, the lineages involved are either allopatric or parapatric\xe2\x80\x94conditions where species delimitation is inherently subjective and particularly dependent on the species concept that has been adopted. These observations suggest that species-level non-monophyly in COI gene trees is less common than previously supposed, with many cases reflecting misidentifications, the subjectivity of species delimitation or other operational factors.
    Keywords: DNA barcoding ; gene tree ; Lepidoptera ; mitochondrial COI ; mitochondrial cox1 ; paraphyly ; polyphyly ; species delimitation ; species monophyly
    Repository Name: National Museum of Natural History, Netherlands
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  • 8
    Publication Date: 2024-01-12
    Description: Background: Host association patterns in Ectoedemia (Lepidoptera: Nepticulidae) are also encountered in other insect groups with intimate plant relationships, including a high degree of monophagy, a preference for ecologically dominant plant families (e.g. Fagaceae, Rosaceae, Salicaceae, and Betulaceae) and a tendency for related insect species to feed on related host plant species. The evolutionary processes underlying these patterns are only partly understood, we therefore assessed the role of allopatry and host plant family shifts in speciation within Ectoedemia. Methodology: Six nuclear and mitochondrial DNA markers with a total aligned length of 3692 base pairs were used to infer phylogenetic relationships among 92 species belonging to the subgenus Ectoedemia of the genus Ectoedemia, representing a thorough taxon sampling with a global coverage. The results support monophyletic species groups that are congruent with published findings based on morphology. We used the obtained phylogeny to explore host plant family association and geographical distribution to investigate if host shifts and allopatry have been instrumental in the speciation of these leafmining insects. Significance: We found that, even though most species within species groups commonly feed on plants from one family, shifts to a distantly related host family have occasionally occurred throughout the phylogeny and such shifts are most commonly observed towards Betulaceae. The largest radiations have occurred within species groups that feed on Fagaceae, Rosaceae, and Salicaceae. Most species are restricted to one of the seven global biogeographic regions, but within species groups representatives are commonly found in different biogeographic regions. Although we find general patterns with regard to host use and biogeography, there are differences between clades that suggest that different drivers of speciation, and perhaps drivers that we did not examine, have shaped diversity patterns in different clades.
    Keywords: Ectoedemia ; moths ; Nepticulidae ; plant relationships ; host shift ; leafminers
    Repository Name: National Museum of Natural History, Netherlands
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  • 9
    Publication Date: 2024-01-12
    Description: Antispila treitschkiella (Fischer von R\xc3\xb6slerstamm, 1843) and A. petryi Martini, 1899, sp. rev. were regarded as synonymous since 1978, but are shown to be two clearly separated species with different hostplants, life histories, DNA barcodes and morphology. Antispila treitschkiella feeds on Cornus mas L., is bivoltine, and has, by following its ornamentally planted host, greatly expanded its range in north-western Europe. In contrast A. petryi feeds on the widespread native C. sanguinea L., is univoltine, and is one of only two Antispila species previously resident in the British Isles, the Netherlands and northern Europe. Consequently, the increase in abundance of A. treitschkiella in the Netherlands since the early 1990s and in Great Britain in recent years must be regarded as part of a recent expansion into north-western Europe, whereas the native A. petryi is hardly expanding and less abundant. In Britain, detailed surveys of parks and living collections confirmed the monophagy of these two species. A search of British herbarium samples provided no evidence for an earlier date of establishment. Information on recognition of all stages, including DNA barcodes, and distribution is provided, and these two species are compared with the third European Cornus L. leafminer, A. metallella (Denis & Schifferm\xc3\xbcller, 1775).
    Keywords: leaf miners ; moths ; Antispila ; new species
    Repository Name: National Museum of Natural History, Netherlands
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  • 10
    Publication Date: 2024-01-12
    Description: Nepticulidae represent one of the early diverging Lepidoptera lineages, and the family currently comprises over 850 described species. The larvae of the vast majority of the species are leaf miners on Angiosperms and highly monophagous, which has led to persistent ideas on coevolution with their plant hosts. We present here a molecular phylogeny based on eight gene fragments from 355 species, representing 20 out of 22 extant Nepticulidae genera. Using two fossil calibration points, we performed molecular dating to place the origin of the family in the Early Cretaceous, before the main Angiosperm diversification. Based on our results we propose a new classification, abandoning all ranks between family and genus, aswell as subgenera to allowfor a stable classification. The position of Enteucha Meyrick within Nepticulidae remains somewhat ambiguous, and the species-rich cosmopolitan genus Stigmella Schrank, with nearly half of all described Nepticulidae, requires further study. Ectoedemia Busck, Zimmermannia Hering, Acalyptris Meyrick, Etainia Beirne, Parafomoria Borkowski, Muhabbetana Ko\xc3\xa7ak & Kemal and Fomoria Beirne appear to have diversified in a relatively short evolutionary period, leading to short branches in the molecular phylogeny and unclear suprageneric relations. Otherwise support values throughout the phylogeny are mostly high and the species groups, genera and higher clades are discussed in respect of their supporting morphological and life-history characters. Wing venation characters are confirmed to be mostly reliable and relevant for Nepticulidae classification, but some other previously used characters require reinterpretation. The species groups of most genera are recovered, but only partly so in the large genus Stigmella. The molecular dating results are compared with existing knowledge on the timing of the Angiosperm radiation and reveal that the diversification of Nepticulidae could largely have been contemporaneous with their hosts, although some of the genera restricted to a single plant family appear to have begun to diversify before their hosts.
    Keywords: leaf miners ; Angiosperms ; classification
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
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