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  • Artikel  (810)
  • 2020-2022  (810)
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  • 1
    Publikationsdatum: 2020-07-01
    Print ISSN: 0277-3791
    Digitale ISSN: 1873-457X
    Thema: Geographie , Geologie und Paläontologie
    Publiziert von Elsevier
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Publikationsdatum: 2020-06-19
    Beschreibung: Previously, we showed that 3% (31/1032)of asymptomatic healthcare workers (HCWs) from a large teaching hospital in Cambridge, UK, tested positive for SARS-CoV-2 in April 2020. About 15% (26/169) HCWs with symptoms of coronavirus disease 2019 (COVID-19) also tested positive for SARS-CoV-2 (Rivett et al., 2020). Here, we show that the proportion of both asymptomatic and symptomatic HCWs testing positive for SARS-CoV-2 rapidly declined to near-zero between 25th April and 24th May 2020, corresponding to a decline in patient admissions with COVID-19 during the ongoing UK ‘lockdown’. These data demonstrate how infection prevention and control measures including staff testing may help prevent hospitals from becoming independent ‘hubs’ of SARS-CoV-2 transmission, and illustrate how, with appropriate precautions, organizations in other sectors may be able to resume on-site work safely.
    Digitale ISSN: 2050-084X
    Thema: Biologie , Medizin , Allgemeine Naturwissenschaft
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Publikationsdatum: 2020-10-21
    Beschreibung: Double-stranded DNA viruses use ATP-powered molecular motors to package their genomic DNA. To ensure efficient genome encapsidation, these motors regulate functional transitions between initiation, translocation, and termination modes. Here, we report structural and biophysical analyses of the C-terminal domain of the bacteriophage phi29 ATPase (CTD) that suggest a structural basis for these functional transitions. Sedimentation experiments show that the inter-domain linker in the full-length protein promotes oligomerization and thus may play a role in assembly of the functional motor. The NMR solution structure of the CTD indicates it is a vestigial nuclease domain that likely evolved from conserved nuclease domains in phage terminases. Despite the loss of nuclease activity, fluorescence binding assays confirm the CTD retains its DNA binding capabilities and fitting the CTD into cryoEM density of the phi29 motor shows that the CTD directly binds DNA. However, the interacting residues differ from those identified by NMR titration in solution, suggesting that packaging motors undergo conformational changes to transition between initiation, translocation, and termination. Taken together, these results provide insight into the evolution of functional transitions in viral dsDNA packaging motors.
    Print ISSN: 0305-1048
    Digitale ISSN: 1362-4962
    Thema: Biologie
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Publikationsdatum: 2021-06-07
    Beschreibung: The Last Glacial Maximum (LGM, ∼ 21 000 years ago) has been a major focus for evaluating how well state-of-the-art climate models simulate climate changes as large as those expected in the future using paleoclimate reconstructions. A new generation of climate models has been used to generate LGM simulations as part of the Paleoclimate Modelling Intercomparison Project (PMIP) contribution to the Coupled Model Intercomparison Project (CMIP). Here, we provide a preliminary analysis and evaluation of the results of these LGM experiments (PMIP4, most of which are PMIP4-CMIP6) and compare them with the previous generation of simulations (PMIP3, most of which are PMIP3-CMIP5). We show that the global averages of the PMIP4 simulations span a larger range in terms of mean annual surface air temperature and mean annual precipitation compared to the PMIP3-CMIP5 simulations, with some PMIP4 simulations reaching a globally colder and drier state. However, the multi-model global cooling average is similar for the PMIP4 and PMIP3 ensembles, while the multi-model PMIP4 mean annual precipitation average is drier than the PMIP3 one. There are important differences in both atmospheric and oceanic circulations between the two sets of experiments, with the northern and southern jet streams being more poleward and the changes in the Atlantic Meridional Overturning Circulation being less pronounced in the PMIP4-CMIP6 simulations than in the PMIP3-CMIP5 simulations. Changes in simulated precipitation patterns are influenced by both temperature and circulation changes. Differences in simulated climate between individual models remain large. Therefore, although there are differences in the average behaviour across the two ensembles, the new simulation results are not fundamentally different from the PMIP3-CMIP5 results. Evaluation of large-scale climate features, such as land–sea contrast and polar amplification, confirms that the models capture these well and within the uncertainty of the paleoclimate reconstructions. Nevertheless, regional climate changes are less well simulated: the models underestimate extratropical cooling, particularly in winter, and precipitation changes. These results point to the utility of using paleoclimate simulations to understand the mechanisms of climate change and evaluate model performance.
    Repository-Name: EPIC Alfred Wegener Institut
    Materialart: Article , isiRev
    Format: application/pdf
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Publikationsdatum: 2021-07-26
    Repository-Name: EPIC Alfred Wegener Institut
    Materialart: Article , isiRev
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    facet.materialart.
    Unbekannt
    In:  pjruders@ncsu.edu | http://aquaticcommons.org/id/eprint/14552 | 403 | 2014-02-15 00:05:49 | 14552 | United States National Marine Fisheries Service
    Publikationsdatum: 2021-06-28
    Beschreibung: The Common Octopus, Octopus vulgaris, is an r-selected mollusk found off the coast of North Carolina that interests commercial fishermen because of its market value and the cost-effectiveness of unbaited pots that can catch it. This study sought to: 1) determine those gear and environmental factors that influenced catch rates of octopi, and 2) evaluate the feasibility of small-scale commercial operations for this species. Pots were fished from August 2010 through September 2011 set in strings over hard and sandy bottom in waters from 18 to 30 m deep in Onslow Bay, N.C. Three pot types were fished in each string; octopus pots with- and without lids, and conch pots. Proportional catch was modeled as a function of gear design and environmental factors (location, soak time, bottom type, and sea surface water temperature) using binomially distributed generalized linear models (GLM’s); parsimony of each GLM was assessed with Akaike Information Criteria (AIC). A total of 229 octopi were caught throughout the study. Pots with lids, pots without lids, and conch pots caught an average of 0.15, 0.17, and 0.11 octopi, respectively, with high variability in catch rates for each pot type. The GLM that best fit the data described proportional catch as a function of sea surface temperature, soak time, and station; greatest proportional catches occurred over short soak times, warmest temperatures, and less well known reef areas. Due to operating expenses (fuel, crew time, and maintenance), low catch rates of octopi, and high gear loss, a directed fishery for this species is not economically feasible at the catch rates found in this study. The model fitting to determine factors most influential on catch rates should help fishermen determine seasons and gear soak times thatare likely to maximize catch rates. Potting for octopi may be commercially practical as a supplemental activity when targeting demersal fish species that are found in similar habitats and depth ranges in coastal waters off North Carolina.
    Schlagwort(e): Fisheries ; Management
    Repository-Name: AquaDocs
    Materialart: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 13-20
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    facet.materialart.
    Unbekannt
    In:  http://aquaticcommons.org/id/eprint/8676 | 403 | 2012-06-05 13:30:36 | 8676 | United States National Marine Fisheries Service
    Publikationsdatum: 2021-06-25
    Beschreibung: We compared numbers of strikes, proportions of fish that hooked up after strikes, proportions of fish that stayed on hook (retained) after hook up, and numbers of fish caughtbetween circle and J hooks rigged with dead natural fish bait (ballyhoo)and trolled for three oceanic predator species: dolphinfish (Coryphaena hippurus), yellowfin tuna (Thunnus albacares), and wahoo (Acanthocybium solandri). Interactions were compared between circle and J hooks fished on 75 trips by two user groups (charter and recreational fishermen). Hooks were affixed to three species-specific leader types most commonly fished in this region: monofilament (dolphinfish), fluorocarbon (tuna), andwire (wahoo). Numbers of fish caught per trip and three potential mechanisms that might inf luence numbers caught (i.e., number of strikes, proportion of fish hooked, and proportion retained) were modeled with generalized linear models that considered hook type, leader type, species, user(fishing) group, and wave height as main effects. Hook type was a main effect at the catch level; generally, more fish were caught on J hooks than on circle hooks. The effect ofhook type on strike rates was equivocal. However, J hooks had a greater proportion of hook-ups than did circle hooks. Finally, the proportion of fish retained once hooked was generally equal between hook types. We found similar results when data from additional species were pooled as a “tuna” group and a “mackerel” group. We conclude that J hooks are more effective than circle hooks at the hook-uplevel and result in greater numbers of troll-caught dolphinfish, tunas
    Schlagwort(e): Biology ; Ecology ; Fisheries ; Management
    Repository-Name: AquaDocs
    Materialart: article
    Format: application/pdf
    Format: application/pdf
    Format: 156-175
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    facet.materialart.
    Unbekannt
    In:  http://aquaticcommons.org/id/eprint/8753 | 403 | 2012-06-11 18:33:30 | 8753 | United States National Marine Fisheries Service
    Publikationsdatum: 2021-06-26
    Beschreibung: Commercial bottom trawls often have sweeps to herd fish intothe net. Elevation of the sweeps off the seaf loor may reduce seafloor disturbance, but also reduce herdingeffectiveness. In both field and laboratory experiments, we examined the behavior of flatfish in response to sweeps. We tested the hypotheses that 1) sweeps are more effective atherding flatfish during the day than at night, when fish are unable to see approaching gear, and that 2) elevationof sweeps off the seafloor reduces herding during the day, but not at night. In sea trials, day catches were greater than night catches for four out of six flatfish species examined. The elevation of sweeps 10 cm significantlydecreased catches during the day, but not at night. Laboratory experiments revealed northern rock sole (Lepidopsetta polyxystra) and Pacific halibut (Hippoglossus stenolepis) were more likely to be herdedby the sweep in the light, whereas in the dark they tended to pass under or over the sweep. In the light, elevationof the sweep reduced herding, and more fish passed under the sweep. In contrast, in the dark, sweep elevationhad little effect upon the number of fish that exhibited herding behavior. The results of both field and laboratoryexperiments were consistent with the premise that vision is the principle sensory input that controls fish behavior and orientation to trawl gear, and gear performance will differbetween conditions where flatfish can see, in contrast to where they cannot see, the approaching gear.
    Schlagwort(e): Biology ; Ecology ; Fisheries
    Repository-Name: AquaDocs
    Materialart: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 145-154
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    facet.materialart.
    Unbekannt
    In:  http://aquaticcommons.org/id/eprint/8767 | 403 | 2012-06-10 09:01:56 | 8767 | United States National Marine Fisheries Service
    Publikationsdatum: 2021-06-27
    Beschreibung: Diet, gastric evacuation rates, daily ration, and population-level prey demand of bluefin tuna (Thunnus thynnus) were estimated in the continental shelf waters off North Carolina. Bluefin tuna stomachs were collected from commercial fishermen during the late fall and winter monthsof 2003–04, 2004–05, and 2005–06. Diel patterns in mean gut fullness values were used to estimate gastric evacuation rates. Daily ration determined from mean gut fullness values and gastric evacuation rates was used, along with bluefin tuna population size and residency times, to estimate population-level consumption by bluefin tuna on Atlantic menhaden (Brevoortia tyrannus). Bluefin tuna diet (n= 448) was dominated by Atlantic menhaden; other teleosts,portunid crabs, and squid were of mostly minor importance. The time required to empty the stomach after peak gut fullness was estimated to be ~20 hours. Daily ration estimates were approximately 2% of body weight per day. At current western Atlantic population levels, bluefin tuna predation on Atlantic menhaden is minimal compared to predation by other known predators and the numbers taken in commercial harvest. Bluefin tuna appear to occupy coastal waters in North Carolina during winter to prey upon Atlantic menhaden. Thus, changes in the Atlantic menhadenstock status or distribution would alter the winter foraging locations of bluefin
    Schlagwort(e): Biology ; Ecology ; Fisheries
    Repository-Name: AquaDocs
    Materialart: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 56-69
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    facet.materialart.
    Unbekannt
    In:  http://aquaticcommons.org/id/eprint/15563 | 8 | 2014-11-06 00:58:30 | 15563
    Publikationsdatum: 2021-07-08
    Beschreibung: Pacific sea surface temperatures (SSTs) are examined for their associations with (1) summer rainfall, and (2) the latitude location of the mid-tropospheric subtropical high pressure ridge (STR) in the southwestern United States during 1945 to 1986. Extreme northward (southward) displacements of STR are associated with wet (dry) summers over Arizona and an enhanced (weakened) gradient of SST off the California and Baja coasts. These tend to follow winters marked by positive (negative) phases of the PNA, Pacific/North America, teleconnection pattern. Recent decadal variations of Arizona summer rainfall (1950s wet; 1970s dry) appear similarly related to southwestern United States synoptic circulation and eastern Pacific SSTs.
    Schlagwort(e): Atmospheric Sciences ; Oceanography ; PACLIM
    Repository-Name: AquaDocs
    Materialart: conference_item
    Format: application/pdf
    Format: application/pdf
    Format: 55-60
    Standort Signatur Erwartet Verfügbarkeit
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