ISSN:
1573-1111
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract New oligosaccharide-branched β-cyclodextrins having a various oligosaccharides in a primary hydroxyl group of β-cyclodextrin(CD) were synthesized and examined for the interaction with the immobilized concanavalin A(ConA) compared with 6-O-glucosyl and maltosyl CD. Oligosaccharides were converted to lactones at the reducing end, which was connected with 6-monoamino-β-CD forming an amide bond. For the analysis of the interaction between ConA immobilized on an aminosilane-hydrogel surface and various oligosaccharide CDs, a biosensor of the FISONS IAsys apparatus based on a resonant mirror detector (RMD) was used. As a result, it interacted with immobilized ConA and both maltosyl-β-CD(3) and maltosyl-γ-CD(6), and glucosylglucono-amide-β-CD(7) with the association constants, Ka of 134, 833 and 8730 M−1, respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01041538
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