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  • 1
    Publication Date: 2014-10-02
    Description: Galectin-3 has been reported to regulate the functions of a number of immune cell types. We previously reported that galectin-3 is translocated to immunological synapses in T cells upon T-cell receptor engagement, where it associates with ALG-2-interacting protein X (Alix). Alix is known to coordinate with the endosomal sorting complex required for transport (ESCRT) to promote human immunodeficiency virus (HIV)-1 virion release. We hypothesized that galectin-3 plays a role in HIV-1 viral budding. Cotransfection of cells of the Jurkat T line with galectin-3 and HIV-1 plasmids resulted in increased HIV-1 budding, and suppression of galectin-3 expression by RNAi in Hut78 and primary CD4+ T cells led to reduced HIV-1 budding. We used immunofluorescence microscopy to observe the partial colocalization of galectin-3, Alix and Gag in HIV-1-infected cells. Results from co-immunoprecipitation experiments indicate that galectin-3 expression promotes Alix-Gag p6 association, whereas the results of Alix knockdown suggest that galectin-3 promotes HIV-1 budding through Alix. HIV-1 particles released from galectin-3-expressing cells acquire the galectin-3 protein in an Alix-dependent manner, with proteins primarily residing inside the virions. We also found that the galectin-3 N-terminal domain interacts with the proline-rich region of Alix. Collectively, these results suggest that endogenous galectin-3 facilitates HIV-1 budding by promoting the Alix-Gag p6 association.
    Print ISSN: 0959-6658
    Electronic ISSN: 1460-2423
    Topics: Biology , Medicine
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  • 2
    Publication Date: 2016-07-30
    Description: We present emission-line templates for passively-evolving (‘retired’) galaxies, useful for investigation of the evolution of the interstellar medium in these galaxies, and characterization of their high-temperature source populations. The templates are based on high signal-to-noise (〉800) co-added spectra (3700–6800 Å) of ~11 500 gas-rich Sloan Digital Sky Survey galaxies devoid of star formation and active galactic nuclei. Stacked spectra are provided for the entire sample and sub-samples binned by mean stellar age. In our previous paper, Johansson et al., these spectra provided the first measurements of the He ii 4686 Å line in passively-evolving galaxies, and the observed He ii /Hβ ratio constrained the contribution of accreting white dwarfs (the ‘single-degenerate’ scenario) to the Type Ia supernova rate. In this paper, the full range of unambiguously detected emission lines are presented. Comparison of the observed [O i ] 6300 Å/Hα ratio with photoionization models further constrains any high-temperature single-degenerate scenario for Type Ia supernovae (with 1.5 T /10 5 K 10) to 3–6 per cent of the observed rate in the youngest age bin (i.e. highest SN Ia rate). Hence, for the same temperatures, in the presence of an ambient population of post-asymptotic giant branch stars, we exclude additional high-temperature sources with a combined ionizing luminosity of 1.35  x  10 30 L /M ,* for stellar populations with mean ages of 1–4 Gyr. Furthermore, we investigate the extinction affecting both the stellar and nebular continuum. The latter shows about five times higher values. This contradicts isotropically distributed dust and gas that renders similar extinction values for both cases.
    Print ISSN: 0035-8711
    Electronic ISSN: 1365-2966
    Topics: Physics
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  • 3
    Publication Date: 2016-04-12
    Description: The Yuan-Agrawal (YA) memory-free approach is employed to study fractional dynamical systems with freeplay nonlinearities subjected to a harmonic excitation, by combining it with the precise integration method (PIM). By the YA method, the original equations are transformed into a set of first-order piecewise-linear ordinary differential equations (ODEs). These ODEs are further separated as three linear inhomogeneous subsystems, which are solved by PIM together with a predictor-corrector process. Numerical examples show that the results by the presented method agree well with the solutions obtained by the Runge-Kutta method and a modified fractional predictor-corrector algorithm. More importantly, the presented method has higher computational efficiency.
    Print ISSN: 1024-123X
    Electronic ISSN: 1563-5147
    Topics: Mathematics , Technology
    Published by Hindawi
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  • 4
    Publication Date: 2015-07-17
    Description: The electrochemical corrosion behavior of A3 in compound sodium molybdate and organic inhibitor solution was tested by the electrochemical workstation method. The concentration of the compound inhibitor set to range 250 mg/L to 3000 mg/L. The polarization curve results of A3 in different concentration inhibitor solutions show that the inhibitor markedly represses the anodic processes. The EIS has two time constant. The extreme concentration is 1500 mg/L.
    Print ISSN: 1757-8981
    Electronic ISSN: 1757-899X
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 5
    Publication Date: 2014-01-16
    Description: For over 200 km along strike the Shuilikeng fault of Taiwan separates Miocene rocks of the Western Foothills from the largely Eocene and Oligocene rocks of the Hsuehshan Range to the east. Despite its importance in the Taiwan mountain belt, the structure and kinematics of the Shuilikeng fault are not well known. Here, we present results from new geological mapping along 100 km of its strike length. At the surface, the Shuilikeng fault is a steeply east-dipping brittle fault with a series of splays and bifurcations. Along its southern part, it cuts an earlier fold and fault system. Outcrop kinematic data vary widely, from thrusting to strike-slip. The surface data are integrated with a relocated and collapsed seismicity database to interpret the fault location at depth. These data indicate that the Shuilikeng fault can be traced to greater than 20 km depth. Some 260 focal mechanisms from this dataset indicate that its kinematics is overall transpressive. From a regional perspective, we interpret the Shuilikeng fault to reactivate a pre-existing rift-related basin-bounding fault to the east of which rocks in the Hsuehshan Range are being exhumed.
    Print ISSN: 0016-7649
    Topics: Geosciences
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  • 6
    Publication Date: 2013-10-25
    Print ISSN: 0895-0695
    Electronic ISSN: 1938-2057
    Topics: Geosciences
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  • 7
    Publication Date: 2014-08-27
    Description: The NaCl-type La monopnictides are proper reference materials for the study of strongly correlated rare-earth pnictides. Yet, despite the simple crystal structure of this system, traditional density functional theory (DFT) calculations have dramatic failures in describing their electronic properties: DFT severely underestimates the band gaps and thus predicts incorrect transport characters of them. Here, we perform a corrected DFT calculation to rectify this failure. Our results show that LaN, LaP, and LaAs are semiconductor with band gaps of 0.82, 0.25, and 0.12 eV, respectively, and LaSb is semimetallic with an overlap of conduction and valence bands approximately 0.28 eV, in agreement with the available experiments. Additionally, under high-pressure, we find that LaN displays a new sequence of phase-transition, B1 → anti-B10 → B2, which is different from the previous theoretical predictions but consistent with the recent experiment.
    Print ISSN: 0021-8979
    Electronic ISSN: 1089-7550
    Topics: Physics
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  • 8
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1990-12-14
    Description: Mutations of the gene encoding p53, a 53-kilodalton cellular protein, are found frequently in human tumor cells, suggesting a crucial role for this gene in human oncogenesis. To model the stepwise mutation or loss of both p53 alleles during tumorigenesis, a human osteosarcoma cell line, Saos-2, was used that completely lacked endogenous p53. Single copies of exogenous p53 genes were then introduced by infecting cells with recombinant retroviruses containing either point-mutated or wild-type versions of the p53 cDNA sequence. Expression of wild-type p53 suppressed the neoplastic phenotype of Saos-2 cells, whereas expression of mutated p53 conferred a limited growth advantage to cells in the absence of wild-type p53. Wild-type p53 was phenotypically dominant to mutated p53 in a two-allele configuration. These results suggest that, as with the retinoblastoma gene, mutation of both alleles of the p53 gene is essential for its role in oncogenesis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chen, P L -- Chen, Y M -- Bookstein, R -- Lee, W H -- CA51495/CA/NCI NIH HHS/ -- EY00278/EY/NEI NIH HHS/ -- EY05758/EY/NEI NIH HHS/ -- New York, N.Y. -- Science. 1990 Dec 14;250(4987):1576-80.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pathology, School of Medicine, University of California, San Diego, La Jolla 92093-0612.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2274789" target="_blank"〉PubMed〈/a〉
    Keywords: Alleles ; Base Sequence ; *Cinnamates ; Cloning, Molecular ; DNA/genetics ; Drug Resistance/genetics ; Genes, p53/*genetics ; Genetic Vectors ; Humans ; Hygromycin B/analogs & derivatives ; Molecular Sequence Data ; Moloney murine leukemia virus/genetics ; Mutation ; Neomycin ; Osteosarcoma/*genetics ; Plasmids ; Transfection ; Tumor Cells, Cultured
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 9
    Publication Date: 1988-12-23
    Description: The aminoacylation specificity ("acceptor identity") of transfer RNAs (tRNAs) has previously been associated with the position of particular nucleotides, as opposed to distinctive elements of three-dimensional structure. The contribution of a G.U wobble pair in the acceptor helix of tRNA(Ala) to acceptor identity was examined with synthetic amber suppressor tRNAs in Escherichia coli. The acceptor identity was not affected by replacing the G.U wobble pair in tRNA(Ala) with a G.A, C.A, or U.U wobble pair. Furthermore, a tRNA(Ala) acceptor identity was conferred on tRNA(Lys) when the same site in the acceptor helix was replaced with any of several wobble pairs. Additional data with tRNA(Ala) show that a substantial acceptor identity was retained when the G.U wobble pair was translocated to another site in the acceptor helix. These results suggest that the G.U wobble pair induces an irregularity in the acceptor helix of tRNA(Ala) to match a complementary structure in the aminoacylating enzyme.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉McClain, W H -- Chen, Y M -- Foss, K -- Schneider, J -- GM42123/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1988 Dec 23;242(4886):1681-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Bacteriology, University of Wisconsin, Madison 53706.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2462282" target="_blank"〉PubMed〈/a〉
    Keywords: Base Composition ; Escherichia coli/*genetics ; Mutation ; *Nucleic Acid Conformation ; RNA, Bacterial/*metabolism ; RNA, Transfer, Ala/*metabolism ; RNA, Transfer, Amino Acid-Specific/*metabolism ; Structure-Activity Relationship ; Suppression, Genetic
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 10
    Publication Date: 2016-05-27
    Description: Quiescent galaxies with little or no ongoing star formation dominate the population of galaxies with masses above 2 x 10(10) times that of the Sun; the number of quiescent galaxies has increased by a factor of about 25 over the past ten billion years (refs 1-4). Once star formation has been shut down, perhaps during the quasar phase of rapid accretion onto a supermassive black hole, an unknown mechanism must remove or heat the gas that is subsequently accreted from either stellar mass loss or mergers and that would otherwise cool to form stars. Energy output from a black hole accreting at a low rate has been proposed, but observational evidence for this in the form of expanding hot gas shells is indirect and limited to radio galaxies at the centres of clusters, which are too rare to explain the vast majority of the quiescent population. Here we report bisymmetric emission features co-aligned with strong ionized-gas velocity gradients from which we infer the presence of centrally driven winds in typical quiescent galaxies that host low-luminosity active nuclei. These galaxies are surprisingly common, accounting for as much as ten per cent of the quiescent population with masses around 2 x 10(10) times that of the Sun. In a prototypical example, we calculate that the energy input from the galaxy's low-level active supermassive black hole is capable of driving the observed wind, which contains sufficient mechanical energy to heat ambient, cooler gas (also detected) and thereby suppress star formation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cheung, Edmond -- Bundy, Kevin -- Cappellari, Michele -- Peirani, Sebastien -- Rujopakarn, Wiphu -- Westfall, Kyle -- Yan, Renbin -- Bershady, Matthew -- Greene, Jenny E -- Heckman, Timothy M -- Drory, Niv -- Law, David R -- Masters, Karen L -- Thomas, Daniel -- Wake, David A -- Weijmans, Anne-Marie -- Rubin, Kate -- Belfiore, Francesco -- Vulcani, Benedetta -- Chen, Yan-mei -- Zhang, Kai -- Gelfand, Joseph D -- Bizyaev, Dmitry -- Roman-Lopes, A -- Schneider, Donald P -- England -- Nature. 2016 May 25;533(7604):504-8. doi: 10.1038/nature18006.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Kavli Institute for the Physics and Mathematics of the Universe (World Premier International Research Center Initiative), The University of Tokyo Institutes for Advanced Study, The University of Tokyo, Kashiwa, Chiba 277-8583, Japan. ; Sub-department of Astrophysics, Department of Physics, University of Oxford, Denys Wilkinson Building, Keble Road, Oxford OX1 3RH, UK. ; Institut d'Astrophysique de Paris (UMR 7095, CNRS and UPMC), 98 bis Boulevard Arago, F-75014 Paris, France. ; Department of Physics, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok 10330, Thailand. ; Institute for Cosmology and Gravitation, University of Portsmouth, Dennis Sciama Building, Burnaby Road, Portsmouth PO1 3FX, UK. ; Department of Physics and Astronomy, University of Kentucky, 505 Rose Street, Lexington, Kentucky 40506-0055, USA. ; Department of Astronomy, University of Wisconsin-Madison, 475 North Charter Street, Madison, Wisconsin 53706, USA. ; Department of Astrophysical Sciences, Princeton University, Princeton, New Jersey 08544, USA. ; Center for Astrophysical Sciences, Department of Physics and Astronomy, The Johns Hopkins University, Baltimore, Maryland 21218, USA. ; McDonald Observatory, Department of Astronomy, University of Texas at Austin, 1 University Station, Austin, Texas 78712-0259, USA. ; Space Telescope Science Institute, 3700 San Martin Drive, Baltimore, Maryland 21218, USA. ; Department of Physical Sciences, The Open University, Milton Keynes MK7 6AA, UK. ; School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, Fife KY16 9SS, UK. ; Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, Massachusetts 02138, USA. ; Cavendish Laboratory, University of Cambridge, 19 J. J. Thomson Avenue, Cambridge CB3 0HE, UK. ; Kavli Institute for Cosmology, University of Cambridge, Cambridge CB3 0HE, UK. ; Department of Astronomy, Nanjing University, Nanjing 210093, China. ; New York University Abu Dhabi, PO Box 129188, Abu Dhabi, United Arab Emirates. ; Center for Cosmology and Particle Physics, New York University, Meyer Hall of Physics, 4 Washington Place, New York, New York 10003, USA. ; Apache Point Observatory and New Mexico State University, PO Box 59, Sunspot, New Mexico 88349-0059, USA. ; Sternberg Astronomical Institute, Moscow State University, Moscow, Russia. ; Departamento de Fisica y Astronomia, Facultad de Ciencias, Universidad de La Serena, Cisternas 1200, La Serena, Chile. ; Department of Astronomy and Astrophysics, The Pennsylvania State University, University Park, Pennsylvania 16802, USA. ; Institute for Gravitation and the Cosmos, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/27225122" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
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