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  • 1
    ISSN: 1520-4804
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1520-4804
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 10 (1967), S. 329-335 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Wir untersuchten das Verteilungsmuster von unspezifischer Esterase, alkalischer Phosphatase, Adenosintriphosphatase, 5′-Nucleotidase und β-D-Glucuronidase im Hoden von Hund und Katze. Besonders hervorzuheben sind eine starke Aktivität der unspezifischen Esterase in den Sertolizellen der Katze, der Reichtum der Membrana propria aller Hodentubuli an alkalischer Phosphatase und Adenosintriphosphatase sowie die kräftige Reaktion auf β-D-Glucuronidase in den Leydigzellen beider Tierarten. Die Befunde werden diskutiert.
    Notes: Summary The localization of unspecific esterase, alkaline phosphatase, adenosine triphosphatase, 5′-nucleotidase, and β-D-glucuronidase in the testes of cat and dog was demonstrated by histochemical means. We observed a strong esterase activity in the Sertoli cells of the cat and high amounts of alkaline phosphatase and adenosine triphosphatase in the membrana propria of all seminiferous tubules. In both species the principal site of β-D-glucuronidase was in the Leydig cells. Our findings obtained being discussed.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 8 (1967), S. 315-326 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung In den Glandulae inguinales 4 geschlechtsreifer Kaninchen (2♂, 2♀) wurden folgende Enzyme mit histochemischen Methoden nachgewiesen: Alkalische Phosphatase, Adenosintriphosphatase, Glucose-6-phosphatase, 5′-Nukleotidase, unspezifische Esterase, β-D-Glucuronidase und β-D-Galactosidase. Die Histotopik dieser Enzyme in der braunen und weißen Inguinaldrüse des Kaninchens zeigt in zahlreichen Punkten eine weitgehende Übereinstimmung mit dem Enzymverteilungsbild in den Schweiß- und Talgdrüsen der Haut. Die Befunde werden diskutiert.
    Notes: Summary In the glandulae inguinales of 4 adult rabbits (2 male, 2 female) the distribution of alkaline phosphatase, adenosintriphosphatase, glucose-6-phosphatase, 5′-nucleotidase, unspecific esterase, β-D-glucuronidase and β-D-galactosidase was studied by histochemical means. The site of enzymatic activity in the white and in the brown inguinal gland of the rabbit shows a condition very similar to that found in the sweat glands and the sebaceous glands of the skin. The findings obtained being discussed.
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  • 5
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The innervation pattern of the bovine deferent duct was studied by acetylcholinesterase (AChE)-histochemistry and by immunohistochemical methods. Using antibodies against protein gene product-9.5 (PGP-9.5) and neuron specific enolase (NSE) the complete innervation pattern can be visualized. Thick nerve bundles in the periductal connective tissue supply the two-layered muscular coat. The inner, mainly circularly arranged muscle bundles are innervated by a particularly dense plexus, whereas the nervous network of the more longitudinally running outer musculature is somewhat looser. Additionally, nerve fibres were observed in the subepithelial space in connection with blood vessels and in close proximity to the basal lamina. An innervation pattern analogous to that of the two panneuronal markers was displayed in the immunoreaction against dopamine-beta-hydroxylase (DBH), indicating that the innervation of the bovine deferent duct is predominantly adrenergic. However, the positive reaction with a monoclonal antibody against cholinacetyltransferase (ChAT) specifically demonstrated for the first time the presence of a cholinergic nerve plexus, restricted to the inner muscular layer and the subepithelial space. A modified, direct-colouring AChE-method is presented, which uses copper chloride as source of cupric ions, acetylthiocholine chloride as substrate and 2-morpholinoethanesulphonic acid (MES) as buffer. After short incubation (1–2 h) our modified method allows the specific visualization of cholinergic nerves, comparable to the results of ChAT-immunoreactivity; following a long incubation time (24 h), it reliably illustrates the autonomous innervation pattern as completely as immunohistochemical panneuronal markers.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 10 (1967), S. 201-207 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung In den Glandulae inguinales 4 geschlechtsreifer Kaninchen (2 männliche, 2 weibliche) werden mehrere Enzyme der Glykolyse und ihrer Seitenwege, des Citronensäure-Zyklus und der Atmungskette histochemisch nachgewiesen. Es zeigt sich, daß sowohl in der Histotopik als auch in der Aktivität dieser Enzyme Parallelen zu dem Enzymverteilungsbild in apokrinen Duft- und Talgdrüsen der Haut bestehen. Die Befunde werden diskutiert.
    Notes: Summary In the inguinal glands of 4 adult rabbits (2 males, 2 females) the distribution pattern of some dehydrogenases and of cytochrome oxydase is described. In the brown lobe of the inguinal gland activity and localization of the enzymes studied parallel the condition found in mammalian apocrine scent glands. The white lobe of the inguinal gland is according to morphological and histochemical features an enlarged and specialized sebaceous gland.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 10 (1967), S. 208-215 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Im Hoden von Hund und Katze werden folgende Enzyme histochemisch nachgewiesen: NADH-Tetrazoliumreduktase (NADH-T-Red), NADPH-Tetrazoliumreduktase (NADPH-T-Red), Cytochromoxydase (Cyt-Ox), Lactat-Dehydrogenase (LDH), Aldolase (ALD), Alkohol-Dehydrogenase (ADH), Glycerin-1-phosphat-Dehydrogenase (GDH), Glucose-6-phosphat-Dehydrogenase (G-6-PDH), Succinat-Dehydrogenase (SDH), NAD-spezifische Isocitrat-Dehydrogenase (NAD-ICDH). Die starke Fermentaktivität der G-6-PDH und der LDH in den Leydig-Zellen beider Spezies, der relativ hohe Gehalt an histochemisch nachweisbarer ADH in den Zwischenzellen der Katze sowie eine deutliche Reaktion auf GDH in den Sertoli-Zellen der Katze werden diskutiert.
    Notes: Summary In the testes of dog and cat the distribution pattern of NADH-tetrazolium reductase, NADPH-tetrazolium reductase, cytochrome oxydase, lactate dehydrogenase, aldolase, alcohol dehydrogenase, α-glycerophosphate dehydrogenase, glucose-6-phosphate dehydrogenase, succinate dehydrogenase and NAD specific isocitrate dehydrogenase was studied by histochemical means. The strong reaction of G-6-PDH and LDH in the Leydig cells of both species, the relatively high amount of ADH in the interstitial cells of the cat testis and the principal site of α-GPDH in the Sertoli cells of the cat are discussed.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 7 (1966), S. 245-250 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung In der Harderschen Drüse des Kaninchens wurden mit der Tetrazoliumtechnik die Enzyme Aldolase und Alkohol-Dehydrogenase histochemisch nachgewiesen und ihr Verteilungsbild in den beiden Drüsenlappen studiert. Beide Drüsenanteile zeigen hohe Aktivitäten der genannten Enzyme. Auf Grund dieser und früher mitgeteilten Befunde (Kühnel, 1966) wird die Möglichkeit nicht ausgeschlossen, daß die Hardersche Drüse an der Biosynthese von Steroiden beteiligt ist.
    Notes: Summary The histochemical localization of aldolase (ALD) and alcohol-dehydrogenase (NAD-ADH) was studied in both lobes of the Harder gland in rabbits by means of the tetrazolium technique. Both lobes of the Harder gland were active. The enzyme activities of ALD and ADH and those of G-6-PDH and NAD-T-Red and their functional link with the process of steroidbiosynthesis are discussed.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 108 (1970), S. 582-596 
    ISSN: 1432-0878
    Keywords: Glandula bulbourethralis ; Goat ; Ultrastructure ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Mehrzahl der sezernierenden Zylinderzellen in den tubulösen Endstücken der Glandula bulbourethralis der Ziege sind Schleimzellen. Ihre großen, aufgehellten Sekretgranula, die fast den gesamten Zelleib ausfällen, liegen so dicht, daß sie sich gegenseitig abplatten. Einzelne von ihnen haben ihre Hüllmembran verloren und neigen zur Konfluenz. Alle Schleimkörnchen sind PAS-positiv, viele von ihnen zeigen eine neuraminidaselabile Alcianophilie bei pH 2,5. Neben den Schleimzellen findet man in der Glandula bulbourethralis der Ziege einen zweiten Zelltyp, der durch helle, blasenförmige Kerne, ein stark entfaltetes granuläres endoplasmatisches Retikulum mit dilatierten Zisternen sowie ein ausgedehntes supranukleares Golgi-Feld gekennzeichnet ist. Dieser zweite Zelltyp enthält sehr elektronendichte, isoliert liegende Sekretkugeln, welche lichtmikroskopisch eine Proteinreaktion geben. Zwischen beiden Zelltypen kommen morphologische Übergangsformen vor. Dies macht es wahrscheinlich, daß es sich bei den beiden Typen lediglich um Funktionsstadien einer einzigen Drüsenzelle handelt. Die sezernierende Oberfläche beider Zelltypen ist durch die Ausbildung interzellulärer Sekretkapillaren vergrößert. Diese sind bereits lichtmikroskopisch aufgrund ihrer kräftigen ATPase- und 5′-Nucleotidaseaktivität zu identifizieren. Das gesamte Drüsenparenchym reagiert sehr stark auf unspezifische Esterase und deutlich positiv auf β-D-Galactosidase, β-D-Glucuronidase, Leucinaminopeptidase, Cytochromoxydase und Succinatdehydrogenase. Die letzten 5 Enzyme sind in den Schleimzellen in geringerer Konzentration festzustellen als in den Zellen mit Eiweißgranula.
    Notes: Summary The majority of the secretory cells in the tubular endpieces of the caprine bulbourethral gland are mucous cells. Their closely packed, relatively large secretory granules exhibit a low electron density. Some granules have lost their limiting membrane, this results in the accumulation of irregularly outlined masses of secretory material. All mucous secretory granules are PAS-positive, many of them are characterized by an alcianophilia at pH 2.5 which is extinguished by pre-treatment with a neuraminidase solution. The second type of secretory bulbourethral cell exhibits light, spherical nuclei, a well developed rough endoplasmic reticulum with dilated cisterns and a large supranuclear Golgi-Complex. The cytoplasm contains smaller, highly electron dense granules which are — according to histochemical tests — of protein nature. The existence of transitional forms between both described cell types permits the conclusion that they must be regarded as functional stages of one common gland cell. The secretory surface of both cell types is increased by intercellular canaliculi which can be identified in the light microscope by their strong ATPase and 5′-nucleotidase activities. The entire parenchyma of the gland is site of an exceptionally high esterase concentration. Furthermore, the gland cells contain considerable amounts of β-D-galactosidase, β-D-glucuronidase, leucine aminopeptidase, cytochrome oxydase and succinic dehydrogenase. These last five enzymes are histochemically more active in the protein secreting than in the mucus producing cell type.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 135 (1972), S. 129-148 
    ISSN: 1432-0878
    Keywords: Ductuli efferentes ; Bull ; Absorption ; Apocrine extrusion ; Light and electron microscopy ; Enzyme cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das Epithel der Ductuli efferentes des erwachsenen Bullen ist einschichtig zylindrisch mit gelegentlichen Basalzellen. Die Zylinderzellen lassen sich in Zilienzellen und Hauptzellen untergliedern. Hauptzellen treten vor allem in zwei Funktionsstadien mit unterschiedlichen morphologischen Charakteristika auf: 1. Resorbierende Hauptzellen besitzen einen Saum gleichmäßig langer Mikrovilli, zwischen deren Basen sich ein System aus gewundenen Canaliculi in das apikale Cytoplasma einsenkt. Diese Canaliculi stehen mit „coated“ Vesikeln und größeren resorptiven Vakuolen in Verbindung. Innerhalb der Vakuolen findet ein Abbau von resorbierter testikulärer Flüssigkeit statt. Hieran sind lysosomale Enzyme beteiligt, wie der kräftige Ausfall der histochemischen Reaktionen auf saure Phosphatase, unspezifische Esterasen und β-D-Glucuronidase im Epithel der Ductuli belegt. Unverdaulicher Vakuoleninhalt verdichtet sich zu homogenen,PAS-positiven, eiweißhaltigen, fluoreszierenden, membranlosen, osmiophilen Globuli. 2. Extrudierende Hauptzellen besitzen aufgehellte, mikrovillifreie apikale Cytoplasmaprojektionen, die mit den in ihnen gelegenen Globuli in das Ductuluslumen abgenabelt werden können. Für eine Phagocytose von ganzen oder fragmentierten Spermatozoen durch das Epithel der Ductuli efferentes des Bullen haben sich keine morphologischen Anhaltspunkte ergeben.
    Notes: Summary The ductuli efferentes of the adult bull are lined by a simple columnar epithelium with scattered small basal cells. The columnar epithelium consists of ciliated and non-ciliated cells. Among non-ciliated cells two functional stages with distinct morphological features may be identified: 1. Absorptive cells possess a border of numerous, rather evenly packed microvilli with strong alkaline phosphatase activity. Between the microvilli the apical cell membrane is invaginated to form a system of narrow, coiled canaliculi which are continuous with coated vesicles and large vacuoles. Testicular fluid is reabsorbed into these vacuoles and broken down by means of lysosomal enzymes. So high levels of acid phosphatase, esterases and β-D-glucuronidase are present in the ductuli efferentes epithelium. Undigestible content of absorptive vacuoles is condensed to homogeneous, PAS-positive, protein-positive, osmiophilic, fluorescing, non membrane-bound globules. 2. Non-ciliated cells with light, globules containing projections of the apical cytoplasm. These protrusions are occasionally found pinched off into the ductular lumen. We did not find any morphological evidence for phagocytosis of whole or fragmented spermatozoa by the epithelium of the bovine ductuli efferentes.
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