ISSN:
1076-5174
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Physics
Notes:
The neutral species generated during the collisionally activated dissociation (CAD) of the protonated dipeptides Ala-Leu, Ala-Ile, Leu-Ala and Ile-Ala are post-ionized by collision and detected in neutral fragment-reionization (+NfR+) mass spectra. These spectra are dominated by fragment ions diagnostic for the C-terminal amino acid, as confirmed by comparison with reference collision-induced dissociative ionization (CIDI) mass spectra of authentic Ala, Leu and Ile. CIDI of Leu and Ile yields distinct and structurally indicative products. These are also observed upon +NfR+ of [Ala-Leu]H+ and [Ala-Ile]H+, respectively, thus allowing one to deduce the correct sequence. In contrast, [Leu-Ala]H+ and [Ile-Ala]H+, which possess identical C-termini, form no differentiating neutral losses upon CAD.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jms.1190301007
Permalink