ISSN:
1432-1203
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Twenty-two independent man-mouse (A9, HGPRT-) and manhamster (CH, HGPRT)-hybrids using male human with balanced reciprocal translocation XY t(X;12)(q2,3;q1,2) were analysed for human genes localized on chromosome 12 (LDHB, PepB, ENO2, TPI), on chromosome X (PGK, αGALA, G6PD) and for human chromosomes in relation with the balanced reciprocal translocation (chromosome 12, 12q-, Xq+). The human chromosome 12q- was not analysed because of its morphology is similar to some rodent chromosomes. The following results were obtained: 1.Man-mouse hybrids (12 independent hybrids): The chromosome 12 is absent. A positive correlation is observed between Xq+, PGK, and PepB. Four hybrids are Xq++PGK+PepB+ and eight hybrids are Xq+-PGK-PepB-This result indicate that the genes for human PGK, PepB are on the chromosome Xq+. 2.Man-hamster hybrids (10 independent hybrids): A positive correlation is observed between Xq+, PGK, αGALA. Two hybrids are Xq++PGK+αGALA+ and eight hybrids are Xq+-PGK-αGALA-. A positive correlation is observed between Xq+, PGK, αGALA, PepB with the seven hybrids missing the normal human chromosome 12. One hybrid is Xq++PGK+αGALA+PepB+ and six hybrids are Xq+-PGK-αGALA-PepB-. These results indicate that the genes for human PGK, αGALA, PepB are on the chromosome Xq+. 3.Man-mouse and man-hamster hybrids: The highest percentage of presence in the hybrids is observed for the following markers: G6PD (100%), LDHB, TPI, ENO2 (90%). This is explained by the fact that these hybrids selected in HAT medium, had to retain a segment of X (Xq+ or 12q-) bearing the human HGPRT gene. The different results indicated that the segment of X retained in these hybrids must be on the 12q- and not on the Xq+, for Xq+ is rarely present in man rodent hybrids. The different results implicate finally the following localisations: LDHB, TPI, ENO2 on 12 q12 → 12pter, PepB on 12 q12 → 12qter; PGK, αGALA on X q23 → Xpter; HGPRT, G6PD on X q23 → Xqter.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00283638
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