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  • 1
    Online Resource
    Online Resource
    21st Century Maritime Silk Road: Wave Energy Resource Evaluation (2020)
    Singapore :Springer Nature Singapore :
    Details
    Keywords: Oceanography. ; Water. ; Hydrology. ; Renewable energy sources. ; Climatology. ; Physical geography. ; Ocean Sciences. ; Water. ; Renewable Energy. ; Climate Sciences. ; Earth System Sciences.
    Description / Table of Contents: Introduction -- Research progress of wave energy evaluation -- Temporal-spatial distribution of wave energy in the Maritime Silk Road -- Climatic trend of wave energy in the Maritime Silk Road -- Short-term forecast of wave energy in the Maritime Silk Road -- Long-term projection of wave energy in the Maritime Silk Road -- Wave energy assessment and proposal in the Sri Lankan waters -- Dataset construction of wave energy resources in the Maritime Silk Road -- Prospects of wave energy evaluation.
    Abstract: This book focuses on the evaluation of wave energy in the Maritime Silk Road. Firstly, it compares wave energy and other main energy sources, and then discusses the various disadvantages. It also presents the current research and the difficulties of wave energy evaluation, and systematically analyzes the climatic characteristics of the wave energy, including the temporal–spatial distribution and climatic trend of a series of key factors (e.g. wave power density, availability, richness, stability, energy direction, energy storage). It then describes the design of a short-term forecasting scheme and a long-term projection scheme of wave energy suitable for the Maritime Silk Road, to serve as a plan for the daily operation and long-term development of wave energy. Further, it highlights the wave energy analysis and decision-making in the context of the remote islands and reefs, using Sri Lanka is taken as a case study. Lastly, it presents the first wave energy resource dataset for the Maritime Silk Road. This book is one of a series of publications on the 21st century Maritime Silk Road (shortened as “Maritime Silk Road”) that covers the characteristics of the marine environment and marine new energy, remote islands and reef construction, climate change, early warning of wave disasters, legal escort, marine environment and energy big data construction, contributing to the safe and efficient construction of the Maritime Silk Road. It aims to improve our knowledge of the ocean, and so improve the capacity for marine construction, enhance the viability of remote islands and reefs, ease the energy crisis and protect the ecological environment and improve the quality of life of residents along the Maritime Silk Road, as well as to protect the rights, and interests of the countries and regions participating in the construction of the Maritime Silk Road. This book is a valuable reference resource for decision-makers, researchers, and marine engineers working in the related fields.
    Type of Medium: Online Resource
    Pages: XIX, 170 p. 95 illus., 94 illus. in color. , online resource.
    Edition: 1st ed. 2020.
    ISBN: 9789811509179
    Series Statement: Springer Oceanography,
    URL: https://doi.org/10.1007/978-981-15-0917-9
    DOI: 10.1007/978-981-15-0917-9
    DDC: 551.46
    Language: English
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    E-BOOKS (AWI ONLY)
  • 2
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    Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO2 (2015)
    PANGAEA
    In:  Supplement to: Wei, Lei; Wang, Qing; Ning, Xuanxuan; Mu, Changkao; Wang, Chunlin; Cao, Ruiwen; Wu, Huifeng; Cong, Ming; Li, Fei; Ji, Chenglong; Zhao, Jianmin (2015): Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO2. Comparative Biochemistry and Physiology Part D: Genomics & Proteomics, 13, 16-23, https://doi.org/10.1016/j.cbd.2014.12.001
    Details
    Publication Date: 2024-03-15
    Description: Ocean acidification (OA) has been found to affect an array of normal physiological processes in mollusks, especially posing a significant threat to the fabrication process of mollusk shell. In the current study, the impact of exposure to elevated pCO2 condition was investigated in mantle tissue of Crassostrea gigas by an integrated metabolomic and proteomic approach. Analysis of metabolome and proteome revealed that elevated pCO2 could affect energy metabolism in oyster C. gigas, marked by differentially altered ATP, succinate, MDH, PEPCK and ALDH levels. Moreover, the up-regulated calponin-2, tropomyosins and myosin light chains indicated that elevated pCO2 probably caused disturbances in cytoskeleton structure in mantle tissue of oyster C. gigas. This work demonstrated that a combination of proteomics and metabolomics could provide important insights into the effects of OA at molecular levels.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or 〈 1 m**2); Crassostrea gigas; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Gene name; Laboratory experiment; Mollusca; mRNA gene expression, relative; mRNA gene expression, relative, standard deviation; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; pH, standard deviation; Salinity; Salinity, standard deviation; Single species; South Pacific; Species; Temperate; Temperature, water; Temperature, water, standard deviation; Treatment
    Type: Dataset
    Format: text/tab-separated-values, 540 data points
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    DATA PANGAEA
  • 3
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    Proteomic and metabolomic responses of Pacific oyster Crassostrea gigas to elevated pCO2 exposure (2015)
    PANGAEA
    In:  Supplement to: Wei, Lei; Wang, Qing; Wu, Huifeng; Ji, Chenglong; Zhao, Jianmin (2014): Proteomic and metabolomic responses of Pacific oyster Crassostrea gigas to elevated pCO2 exposure. Journal of Proteomics, 112, 83-94, https://doi.org/10.1016/j.jprot.2014.08.010
    Details
    Publication Date: 2024-03-15
    Description: The gradually increased atmospheric CO2 partial pressure (pCO2) has thrown the carbonate chemistry off balance and resulted in decreased seawater pH in marine ecosystem, termed ocean acidification (OA). Anthropogenic OA is postulated to affect the physiology of many marine calcifying organisms. However, the susceptibility and metabolic pathways of change in most calcifying animals are still far from being well understood. In this work, the effects of exposure to elevated pCO2 were characterized in gills and hepatopancreas of Crassostrea gigas using integrated proteomic and metabolomic approaches. Metabolic responses indicated that high CO2 exposure mainly caused disturbances in energy metabolism and osmotic regulation marked by differentially altered ATP, glucose, glycogen, amino acids and organic osmolytes in oysters, and the depletions of ATP in gills and the accumulations of ATP, glucose and glycogen in hepatopancreas accounted for the difference in energy distribution between these two tissues. Proteomic responses suggested that OA could not only affect energy and primary metabolisms, stress responses and calcium homeostasis in both tissues, but also influence the nucleotide metabolism in gills and cytoskeleton structure in hepatopancreas. This study demonstrated that the combination of proteomics and metabolomics could provide an insightful view into the effects of OA on oyster C. gigas. BIOLOGICAL SIGNIFICANCE: The gradually increased atmospheric CO2 partial pressure (pCO2) has thrown the carbonate chemistry off balance and resulted in decreased seawater pH in marine ecosystem, termed ocean acidification (OA). Anthropogenic OA is postulated to affect the physiology of many marine calcifying organisms. However, the susceptibility and metabolic pathways of change in most calcifying animals are still far from being understood. To our knowledge, few studies have focused on the responses induced by pCO2 at both protein and metabolite levels. The pacific oyster C. gigas, widely distributed throughout most of the world's oceans, is a model organism for marine environmental science. In the present study, an integrated metabolomic and proteomic approach was used to elucidate the effects of ocean acidification on Pacific oyster C. gigas, hopefully shedding light on the physiological responses of marine mollusk to the OA stress.
    Keywords: Alkalinity, total; Animalia; Aragonite saturation state; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or 〈 1 m**2); Coulometric titration; Crassostrea gigas; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Laboratory experiment; Mollusca; mRNA gene expression, relative; mRNA gene expression, relative, standard deviation; North Pacific; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Potentiometric; Protein name; Salinity; Single species; Species; Temperate; Temperature, water; Tissues; Treatment
    Type: Dataset
    Format: text/tab-separated-values, 352 data points
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    DATA PANGAEA
  • 4
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    Seawater carbonate chemistry and immune function of the Pacific oyster against Vibrio splendidus challenge (2018)
    PANGAEA
    In:  Supplement to: Cao, Ruiwen; Wang, Qing; Yang, Dinglong; Liu, Yongliang; Ran, Wen; Qu, Yi; Wu, Huifeng; Cong, Ming; Li, Fei; Ji, Chenglong; Zhao, Jianmin (2018): CO 2 -induced ocean acidification impairs the immune function of the Pacific oyster against Vibrio splendidus challenge: An integrated study from a cellular and proteomic perspective. Science of the Total Environment, 625, 1574-1583, https://doi.org/10.1016/j.scitotenv.2018.01.056
    Details
    Publication Date: 2024-03-15
    Description: Ocean acidification (OA) and pathogenic diseases pose a considerable threat to key species of marine ecosystem. However, few studies have investigated the combined impact of reduced seawater pH and pathogen challenge on the immune responses of marine invertebrates. In this study, Pacific oysters, Crassostrea gigas, were exposed to OA (~2000 ppm) for 28 days and then challenged with Vibrio splendidus for another 72 h. Hemocyte parameters showed that V. splendidus infection exacerbated the impaired oyster immune responses under OA exposure. An iTRAQ-based quantitative proteomic analysis revealed that C. gigas responded differently to OA stress and V. splendidus challenge, alone or in combination. Generally, OA appears to act via a generalized stress response by causing oxidative stress, which could lead to cellular injury and cause disruption to the cytoskeleton, protein turnover, immune responses and energy metabolism. V. splendidus challenge in oysters could suppress the immune system directly and lead to a disturbed cytoskeleton structure, increased protein turnover and energy metabolism suppression, without causing oxidative stress. The combined OA- and V. splendidus-treated oysters ultimately presented a similar, but stronger proteomic response pattern compared with OA treatment alone. Overall, the impaired oyster immune functions caused by OA exposure may have increased the risk of V. splendidus infection. These results have important implications for the impact of OA on disease outbreaks in marine invertebrates, which would have significant economic and ecological repercussions.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or 〈 1 m**2); Crassostrea gigas; Experiment duration; Fold change; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression; Gene expression, standard deviation; Group; Hemocyte count; Hemocyte count, standard deviation; Immunology/Self-protection; Laboratory experiment; Mollusca; Name; North Pacific; Number of expressed proteins; OA-ICC; Ocean Acidification International Coordination Centre; Other; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Percentage; Percentage, standard deviation; pH; pH, standard deviation; Phagocytosis rate; Phagocytosis rate, standard deviation; Potentiometric; Potentiometric titration; Reactive oxygen species production; Reactive oxygen species production, standard deviation; Registration number of species; Salinity; Salinity, standard deviation; Single species; Species; Temperate; Temperature, water; Temperature, water, standard deviation; Treatment; Type; Uniform resource locator/link to reference
    Type: Dataset
    Format: text/tab-separated-values, 1458 data points
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    DATA PANGAEA
  • 5
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    Seawater carbonate chemistry and Cu accumulation and cellular toxicity in the Pacific Oyster Crassostrea gigas (2019)
    PANGAEA
    Details
    Publication Date: 2024-03-20
    Description: Ocean acidification (OA) has been found to increase the release of free Cu2+ in seawater. However, only a handful of studies have investigated the influence of OA on Cu accumulation and cellular toxicity in bivalve species. In this study, Pacific oysters, Crassostrea gigas, were exposed to 25 μg/L Cu2+ at three pH levels (8.1, 7.8 and 7.6) for 14 and 28 days. Physiological and histopathological parameters [(clearance rate (CR), respiration rate (RR), histopathological damage and condition index (CI)), oxidative stress and neurotoxicity biomarkers [superoxide dismutase (SOD) and glutathione transferase (GST) activities, lipid peroxidation (LPO) and acetylcholinesterase (AChE) activity], combined with glycolytic enzyme activities [pyruvate kinase (PK) and hexokinase (HK)] were investigated in C. gigas. The bioconcentration of Cu was increased in soft tissues of Cu-exposed oysters under OA. Our results suggest that both OA and Cu could lead to physiological disturbance, oxidative stress, cellular damage, disturbance in energy metabolism and neurotoxicity in oysters. The inhibited CR, increased glycolytic enzymes activities and decreased CI suggested that the energy metabolism strategy adopted by oysters was not sustainable in the long term. Furthermore, integrated biomarker response (IBR) results found that OA and Cu exposure lead to severe stress to oysters, and co-exposure was the most stressful condition. Results from this study highlight the need to include OA in future environmental assessments of pollutants and hazardous materials to better elucidate the risks of those environmental perturbations.
    Keywords: Acetylcholinesterase activity, standard deviation; Acetylcholinesterase activity, unit per protein mass; Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Behaviour; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Clearance rate; Clearance rate, standard deviation; Coast and continental shelf; Condition index; Condition index, standard deviation; Containers and aquaria (20-1000 L or 〈 1 m**2); Copper; Copper, standard deviation; Crassostrea gigas; Experiment day; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Glutathione S-transferase activity, standard deviation; Glutathione S-transferase activity, unit per protein mass; Hexokinase activity, per protein mass; Hexokinase activity, standard deviation; Inorganic toxins; Integrated biomarker response index; Laboratory experiment; Lipid peroxidation, per protein; Lipid peroxidation, standard deviation; Mollusca; North Pacific; OA-ICC; Ocean Acidification International Coordination Centre; Other metabolic rates; Other studied parameter or process; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; pH, standard deviation; Potentiometric; Potentiometric titration; Pyruvate kinase activity, per protein; Pyruvate kinase activity, standard deviation; Replicates; Respiration; Respiration rate, oxygen; Respiration rate, oxygen, standard deviation; Salinity; Salinity, standard deviation; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Superoxide dismutase activity, standard deviation; Superoxide dismutase activity, unit per protein mass; Temperate; Temperature, water; Temperature, water, standard deviation; Treatment; Type
    Type: Dataset
    Format: text/tab-separated-values, 732 data points
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    DATA PANGAEA
  • 6
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    Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1 (2021)
    Taylor & Francis
    Details
    Publication Date: 2021-01-02
    Print ISSN: 1554-8627
    Electronic ISSN: 1554-8635
    Topics: Biology
    Published by Taylor & Francis
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    PAPER CURRENT
  • 7
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    Chromosomal-level assembly of the blood clam, Scapharca (Anadara) broughtonii, using long sequence reads and Hi-C (2019)
    In:  EPIC3GigaScience, 8(7), ISSN: 2047-217X
    Details
    Publication Date: 2023-06-21
    Description: Abstract Background The blood clam, Scapharca (Anadara) broughtonii, is an economically and ecologically important marine bivalve of the family Arcidae. Efforts to study their population genetics, breeding, cultivation, and stock enrichment have been somewhat hindered by the lack of a reference genome. Herein, we report the complete genome sequence of S. broughtonii, a first reference genome of the family Arcidae. Findings A total of 75.79 Gb clean data were generated with the Pacific Biosciences and Oxford Nanopore platforms, which represented approximately 86× coverage of the S. broughtonii genome. De novo assembly of these long reads resulted in an 884.5-Mb genome, with a contig N50 of 1.80 Mb and scaffold N50 of 45.00 Mb. Genome Hi-C scaffolding resulted in 19 chromosomes containing 99.35% of bases in the assembled genome. Genome annotation revealed that nearly half of the genome (46.1%) is composed of repeated sequences, while 24,045 protein-coding genes were predicted and 84.7% of them were annotated. Conclusions We report here a chromosomal-level assembly of the S. broughtonii genome based on long-read sequencing and Hi-C scaffolding. The genomic data can serve as a reference for the family Arcidae and will provide a valuable resource for the scientific community and aquaculture sector.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , peerRev
    Format: application/pdf
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    PAPER (OA)
  • 8
    Electronic Resource
    Electronic Resource
    Theory of dynamic absorption spectroscopy of nonstationary states. 4. Application to 12-fs resonant impulsive Raman spectroscopy of bacteriorhodopsin (1992)
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 96 (1992), S. 6147-6158 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/j100194a013
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  • 9
    Electronic Resource
    Electronic Resource
    Nonlinear piezoelectric behavior of ceramic bending mode actuators under strong electric fields (1999)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 86 (1999), S. 3352-3360 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The nonlinear electromechanical behavior of cantilevered piezoelectric ceramic bimorph, unimorph, and reduced and internally biased oxide wafer actuators is studied in a wide electric field and frequency range. It is found that under quasistatic condition, linear relationships between actuator tip displacement-electric field, and blocking force-electric field are only valid under weak field driving. With increasing the driving field, electromechanical nonlinearity begins to contribute significantly to the actuator performance because of ferroelectric hysteresis behavior associated with piezoelectric lead zirconate titanate (PZT)-type ceramic materials. The bending resonance frequencies of all these actuators vary with the magnitude of the electric field. The decrease of resonance frequency with electric field is explained by the increase of elastic compliance of PZT ceramic due to elastic nonlinearity. Mechanical quality factors of the actuators also depend on the magnitude of electric field strength. No significant temperature increase is observed when actuators are driven near resonance frequency under high electric field. © 1999 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.371213
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  • 10
    Electronic Resource
    Electronic Resource
    Dependence of electrical properties on film thickness in lanthanum-doped lead zirconate titanate stannate antiferroelectric thin films (1999)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 85 (1999), S. 3753-3758 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Lanthanum-doped lead zirconate titanate stannate antiferroelectric thin films with compositions in antiferroelectric orthorhombic region have been prepared on Pt-buffered Si substrates by the sol–gel method, with the film thickness from 0.1 to 1.0 μm. The dependence of dielectric and ferroelectric properties on film thickness have been studied, with the emphasis on field-induced phase switching. The dielectric constant and maximum polarization decrease with the reduction of film thickness, which are similar to ferroelectric thin films. However, the decrease of film thickness also leads to the decrease of the phase switching fields, the appearance of remanent polarization in the hysteresis loops, and the smearing of antiferroelectric–ferroelectric phase switching. These phenomena are believed to be caused mainly by the stress effect between the film and substrate. Due to thermal mismatch, the films are under high tensile stress after annealing, and the stress effect becomes more significant with the decrease of film thickness. This makes the thinner films more preferable to the ferroelectric phase and causes the retention of ferroelectric phase in the films with thickness less than 0.4 μm. © 1999 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.369744
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