ISSN:
1573-4927
Keywords:
malic enzyme
;
NAD+
;
polymorphism
;
Salmo
;
salmonids
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract Electrophoretic studies of malate oxidoreductases routinely assess variation in two enzymes, malate dehydrogenase (EC 1.1.1.37) and malic enzyme (NADP+) (EC 1.1.1.40). By modification of the standard isozyme staining conditions for these enzymes, we have resolved a new NAD+-preferring, MgCl2-requiring malic enzyme which is indicated to be EC 1.1.1.39. The enzyme was detected in 10 salmonid fish species of the generaSalmo, Salvelinus, andOnchoryhncus. Phenotypic variation indicates that the novel enzyme is tetrameric and coded by a single locus. Inheritance inS. salar follows a single-locus model and the phenotypes are unlinked to polymorphisms fors MDH-3,4* andm MEP-2*, two malate oxidoreductase loci previously shown to be variable in this species.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00554419
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