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  • 1
    Electronic Resource
    Electronic Resource
    The Realities and Challenges of Plant Biotechnology (1990)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 8 (1990), S. 296-301 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Plant biotechnology has often been touted overzealously and prematurely as a cure-all for many agricultural problems, and a panacea for the genetic manipulation and improvement of plants2. There has been a great deal of hype and rhetoric, but little substance. This general lack of objectivity, ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0490-296
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  • 2
    Electronic Resource
    Electronic Resource
    Progress in the Regeneration and Genetic Manipulation of Cereal Crops (1988)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 6 (1988), S. 397-402 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] The economically important cereal and grass crops have generally proved to be notoriously recalcitrant to manipulation in vitro. Regeneration of plants from single cells, a prerequisite for cellular and molecular manipulation, has proven to be especially difficult. Consequently, this group of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0488-397
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  • 3
    Electronic Resource
    Electronic Resource
    Dedication (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 85 (1992), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1992.tb04733.x
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  • 4
    Electronic Resource
    Electronic Resource
    Advances in cereal protoplast research (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 85 (1992), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Beginning in 1986, plants have been regenerated from protoplasts of all of the important cereal species, including wheat, rice, maize, and barley, and grasses such as sugarcane. In addition, somatic hybrids/cybrids as well as transgenic plants with introduced useful agronomic traits have been obtained in several instances. This rapid and impressive progress in the genetic manipulation of cereals has been made possible by two critical technical advances during the past decade: the establishment of embryogenic suspension cultures as a source of totipotent protoplasts and the direct delivery of DNA into protoplasts for genetic transformation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1992.tb04734.x
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  • 5
    Electronic Resource
    Electronic Resource
    In vitro regeneration and genetic manipulation of grasses (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 73 (1988), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Regeneration of plants from cultured cells is an important and essential component of plant biotechnology. Advances in the recovery of plants from cultured cells and protoplasts of grasses, and in genetic transformation provide challenging opportunities for the genetic manipulation and improvement of this most important group of food plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1988.tb05442.x
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrastructural changes in suspension culture cells of Panicum maximum during cryopreservation (1992)
    Springer
    Plant cell reports 11 (1992), S. 169-174 
    Details
    ISSN: 1432-203X
    Keywords: Cryopreservation ; Panicum maximum ; ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A Panicum maximum cell suspension was used to study ultrastructural changes during cryopreservation. Pregrowing the cells in mannitol caused reduction in the vacuolar volume by redistribution of the large central vacuole into a number of smaller vesicles. Invaginations were formed in the plasma membrane of the cells, to accommodate the reduced cell volume. Swelling of organelles occurred during different stages of cryopreservation. The cisternae of the endoplasmic reticulum dilated and formed vesicles. Although some damage was apparent, organelles were still recognizable in cells frozen slowly and freeze-fixed at −10°C. The cells were able to repair such damage within two days in culture, and regained their normal appearance. Cells frozen slowly without any cryoprotection, and cells frozen rapidly by direct immersion into liquid nitrogen after cryoprotection, were lethally damaged by destruction of membranous structures. Osmiophilic granules were found along the plasma membrane of lethally damaged cells, indicating that their formation is a consequence of freeze damage, rather than a mechanism to prevent injury.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232526
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  • 7
    Electronic Resource
    Electronic Resource
    Accelerated production of transgenic wheat (Triticum aestivum L.) plants (1996)
    Springer
    Plant cell reports 16 (1996), S. 12-17 
    Details
    ISSN: 1432-203X
    Keywords: Triticum aestivum L. ; wheat ; transformation ; biolistics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have developed a method for the accelerated production of fertile transgenic wheat (Triticum aestivum L.) that yields rooted plants ready for transfer to soil in 8–9 weeks (56–66 days) after the initiation of cultures. This was made possible by improvements in the procedures used for culture, bombardment, and selection. Cultured immature embryos were given a 4–6 h pre-and 16 h post-bombardment osmotic treatment. The most consistent and satisfactory results were obtained with 30 μg of gold particles/bombardment. No clear correlation was found between the frequencies of transient expression and stable transformation. The highest rates of regeneration and transformation were obtained when callus formation after bombardment was limited to two weeks in the dark, with or without selection, followed by selection during regeneration under light. Selection with bialaphos, and not phosphinothricin, yielded more vigorously growing transformed plantlets. The elongation of dark green plantlets in the presence of 4–5 mg/l bialaphos was found to be reliable for identifying transformed plants. Eighty independent transgenic wheat lines were produced in this study. Under optimum conditions, 32 transformed wheat plants were obtained from 2100 immature embryos in 56–66 days, making it possible to obtain R3 homozygous plants in less than a year.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275440
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  • 8
    Electronic Resource
    Electronic Resource
    Characterization and regeneration of wheat (Triticum aestivum L.) embryogenic cell suspension cultures (1990)
    Springer
    Plant cell reports 8 (1990), S. 714-717 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Stable cell suspension cultures were established from two types of calli (one compact, nodular and embryogenic, the other friable and embryogenic) derived from cultured immature embryos of wheat (cv FLA302). Only aged calli, which had been subcultured for at least 5–8 months, formed suspensions comprised mainly of groups of small, round, densely cytoplasmic, starch-containing cells. Only the embryogenic suspension derived from the aged, compact and nodular callus formed distinct somatic embryos when plated on regeneration media containing IAA and zeatin. Upon subsequent transfer to fresh regeneration medium more than 200 green rooted plants were obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272101
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  • 9
    Electronic Resource
    Electronic Resource
    Plant regeneration from a cryopreserved embryogenic cell suspension of a commercial sugarcane hybrid (Saccharum sp.) (1990)
    Springer
    Plant cell reports 9 (1990), S. 419-423 
    Details
    ISSN: 1432-203X
    Keywords: Cell suspension ; cryopreservation, Gramineae ; Saccharum ; sugarcane ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Efficient plant regeneration was obtained from a cryopreserved embryogenic cell suspension of sugarcane established from leaf derived callus. Pregrowing the cells for three days in MS basal medium supplemented with 0.33 M sorbitol was essential to the process. The cells were cooled at a rate of 0.5°C/min to −40°C and then stored in liquid nitrogen. Thawing was carried out rapidly in water at +40°C, and the cells were then plated without washing onto filter paper discs placed on a semi-solid regeneration medium (MS basal + 3% sucrose + 0.13 mg/1 2,4-D +0.25 mg/1 BAP + 0.25 mg/1 kinetin + 0.25 mg/1 zeatin). The filter paper discs, along with the cells, were transferred to the same, fresh medium after five hours. After 24 hours the cells were scraped off, placed on fresh semi-solid medium and incubated at 28°C in the dark for two weeks before transfer to light. A regeneration efficiency of 92% was obtained (regenerated plants, expressed as a percent of unfrozen control). Plants regenerated from cryopreserved cells, and grown to maturity in the greenhouse, were morphologically identical to regenerated control plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232263
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  • 10
    Electronic Resource
    Electronic Resource
    Stably transformed herbicide resistant callus of sugarcane via microprojectile bombardment of cell suspension cultures and electroporation of protoplasts (1992)
    Springer
    Plant cell reports 11 (1992), S. 494-498 
    Details
    ISSN: 1432-203X
    Keywords: Sugarcane ; cell suspension ; protoplast ; microprojectile bombardment ; electroporation ; GUS ; bar ; PAT ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Stably transformed callus of a hybrid sugarcane cultivar (Saccharum species hybrid, CP72-1210) was achieved following high velocity microprojectile bombardment of suspension culture cells, and electroporation of protoplasts. A three-day old cell suspension culture (SC88) was bombarded with gold particles coated with pBARGUS plasmid DNA containing the ß-glucuronidase (GUS) reporter gene and the bar selectable gene that confers resistance to the herbicide basta. The pBARGUS plasmid was also electroporated into the protoplasts of another cell line (SCPP). Colonies resistant to basta were recovered from both sources. Stable integration of the bar gene in the resistant cell lines was confirmed by Southern analysis. In addition, phosphinothricin acetyltransf erase (PAT) activity was also demonstrated in the transformed cell lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236264
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