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  • 1
    Electronic Resource
    Electronic Resource
    Initiation of neuropathic pain requires lysophosphatidic acid receptor signaling (2004)
    [s.l.] : Nature Publishing Group
    Nature medicine 10 (2004), S. 712-718 
    Details
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Lysophosphatidic acid (LPA) is a bioactive lipid with activity in the nervous system mediated by G-protein-coupled receptors. Here, we examined the role of LPA signaling in the development of neuropathic pain by pharmacological and genetic approaches, including the use of mice lacking the LPA1 ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nm1060
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  • 2
    Electronic Resource
    Electronic Resource
    Open sandwich ELISA: A novel immunoassay based on the interchain interaction of antibody variable region (1996)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 14 (1996), S. 1714-1718 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] We describe an immunoassay that is based on the interchain interaction of separated VL and VH chains from a single chain antibody variable region. In the presence of antigen, the chains reassociate. VL fragments of anti-hen egg lysozyme (HEL) antibody HyHEL-10 were immobilized on microtiter plates. ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt1296-1714
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  • 3
    Electronic Resource
    Electronic Resource
    Antigen Responsive Antibody–Receptor Kinase Chimera (1992)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 10 (1992), S. 430-433 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] We have constructed chimeric receptors, combining murine IgM and the cytoplasmic portion of human epidermal growth factor receptor (EGFR), with the aim of developing a novel immunosensor with antigen–dependent phosphorylation activity. When intact IgM was used, the chimeric receptor showed ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0492-430
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  • 4
    Electronic Resource
    Electronic Resource
    A novel analgesic dipeptide from bovine brain is a possible Met-enkephalin releaser (1979)
    [s.l.] : Nature Publishing Group
    Nature 282 (1979), S. 410-412 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] During purification, bovine brain extracts were injected intracisternally into unanaesthetised mice, using a J-shaped needle3, and analgesia was evaluated by the tail-pinch test using an artery clip (2 mm wide) with a constant pressure of 200 g (rf. 4). All the materials tested were dissolved in ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/282410a0
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  • 5
    Electronic Resource
    Electronic Resource
    Antibodies to rat sperm tail polypeptides recognize Sertoli cell secretory proteins (1989)
    Springer
    Molecular and cellular biochemistry 85 (1989), S. 171-179 
    Details
    ISSN: 1573-4919
    Keywords: outer dense fibers ; keratin-like fibers ; immunogold electron microscopy ; spermatogenesis ; spermiogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract We have previously reported that (a) polyclonal antisera raised against rat Sertoli cell secretory protein S70 and S45-S35 heterodimeric protein recognize outer dense fiber polypeptides from rat sperm tail, and (b) protein S70 is antigenically related to polypeptides S45 and S35, the disulfide-linked components of the heterodimeric protein. We now report that polyclonal antisera generated against three different outer dense fiber polypeptides recognize (a) the putative antigen of the sperm tail and (b) Sertoli cell secretory protein S70 and its antigenically-related polypeptides. Immunogold electron microscopy shows that outer dense fibers of epididymal sperm crossreact with anti-S70 serum as well as with an antiserum raised against the polypeptide D complex of extracted outer dense fibers. Electron microscopy demonstrates that outer dense fibers consist of filamentous, coil-coiled units aligned side-by-side with each other. Results of this study strengthen the antigenic homology between Sertoli cell secretory proteins and outer dense fiber polypeptides of the sperm tail.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00577112
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  • 6
    Electronic Resource
    Electronic Resource
    Antigenic homology between rat sperm tail polypeptides and Sertoli cell secretory proteins (1988)
    Springer
    Molecular and cellular biochemistry 81 (1988), S. 165-176 
    Details
    ISSN: 1573-4919
    Keywords: sperm tail ; immunofluorescence ; Sertoli cell secretory proteins ; antigenic homology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract A high performance liquid chromatographic procedure has been used for the purification of rat Sertoli cell secretory protein S70 and S45-535 heterodimeric protein to determine their role during spermatogenesis. These two proteins display binding affinity for each other and appear antigenically related. We have observed that: 1. S70 and S45-S35 heterodimeric protein coelute during purification, 2. polyclonal antiserum raised against protein S70 recognizes common antigenic determinants in polypeptides S45 and S35, the disulfide-linked components of the heterodimeric protein, and 3. a monoclonal antibody that recognizes polypeptide S35 but does not crossreact with either protein S70 or polypeptide S45, immunoprecipitates the S70/S45-S35 heterodimeric protein complex. In immunofluorescent experiments, antisera raised against protein S70 and polypeptide components of S45-S35 heterodimeric protein immunoreact with two major sperm intracellular structures: the acrosome and periaxonemal outer dense fibers of sperm tail. Immunoreactivity was not detected on the sperm plasma membrane surface of unfixed, living sperm. Outer dense fibers extracted from sperm tails by a combined treatment with cetylthrimethylammonium bromide and 2-mercaptoethanol, yielded a characteristic polypeptide pattern. In immunoblotting experiments, sperm tail polypeptides were recognized by polyclonal antisera raised against Sertoli cell secretory proteins. We conclude that Sertoli cell secretory proteins S70 and S45-S35 heterodimeric protein are antigenically related to each other and to keratin-like polypeptides from sperm tail.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219319
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  • 7
    Electronic Resource
    Electronic Resource
    Cytophysiology of gonadotropin-releasing-hormone neurons in chum salmon (Oncorhynchus keta) forebrain before and after upstream migration (1996)
    Springer
    Cell & tissue research 284 (1996), S. 261-267 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Salmon gonadotropin-releasing hormone ; Immunocytochemistry ; In situ hybridization ; Olfactory system ; Telencephalon ; Terminal nerve ; Salmon homing migration ; Oncorhynchus keta (Teleostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Cytophysiology of gonadotropin-releasing-hormone neurons in chum salmon (Oncorhynchus keta) was examined before and after upstream migration by an immunocytochemical technique with a specific antiserum to salmon gonadotropin-releasing hormone and an in situ hybridization technique with an oligonucleotide encoding salmon gonadotropin-releasing-hormone precursor (pro-salmon gonadotropin-releasing hormone). In the forebrain (olfactory nerve, olfactory bulb, telencephalon, and preoptic area), salmon gonadotropin-releasing hormone-immunoreactive neurons and neurons showing signals for pro-salmon gonadotropin-releasing-hormone mRNA were compared between fish from the coastal sea and those from the spawning ground. Neurons in the dorsal region of the olfactory nerve and in the ventral region of the transitional area between olfactory nerve and olfactory bulb showed strong salmon gonadotropin-releasing-hormone immunoreactivity and strong hybridization signals in fish from the coastal sea, but these activities and signals were not observed or were decreased in number in fish from the spawning ground. The neurons in the olfactory bulb, telencephalon, and preoptic area consistently revealed salmon gonadotropin-releasing-hormone immunoreactivity and hybridization signals, and the hybridization signals of salmon gonadotropin-releasing hormone in the telencephalon and the preoptic area were stronger in fish from the spawning ground than in those from the coastal sea. These findings suggest that salmon gonadotropin-releasing-hormone neurons in the olfactory nerve and the transitional area between olfactory nerve and olfactory bulb have different patterns of hormone production than those in the telencephalon and the preoptic area.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050586
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  • 8
    Electronic Resource
    Electronic Resource
    Reinforcing apoptosis-resistance of COS and myeloma cells by transfecting with bcl-2 gene (1997)
    Springer
    Cytotechnology 25 (1997), S. 25-33 
    Details
    ISSN: 1573-0778
    Keywords: apoptosis ; bcl-2 ; COS cell ; myeloma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract COS, myeloma and HeLa cells, which are commonly used for protein production by cell culture, were transfected with human bcl-2 gene encoded on the shuttle vector BCMGS. Expression of human bcl-2 improved survival of cells remarkably, mildly, or negligibly for COS, myeloma, and HeLa, respectively. Four clones were obtained from the human bcl-2 expressing cell population of COS cells. They expressed human bcl-2 almost at the same level. The viable cell numbers were 6, 2.5, 2.5, and 0.8 times as many for the clones #8, #5, #6, and #7, respectively, as for the control COS cells, when they were cultured at low (0.2%) serum concentration for 9 days. The bcl-2 overexpressing COS cells showed morphology different from that of the control COS cells in serum limited condition. When transfected with mouse lambda protein gene carried by an SV40-derived vector, clone #8 of the bcl-2 transfected COS cells continued the transient expression of lambda protein longer than the control COS cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007935026770
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  • 9
    Electronic Resource
    Electronic Resource
    Erratum: Establishing apoptosis resistant cell lines for improving protein productivity of cell culture (1998)
    Springer
    Cytotechnology 26 (1998), S. 79-79 
    Details
    ISSN: 1573-0778
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Cytotechnology 23 : 55–59, 1997. The correct version of authors list should read: Eiji Suzuki1-, Satoshi Terada1, Hiroshi Ueda1, Tetsuo Fujita1, Tomoaki Komatsu1, Yon Hui Kim1, Shinichi Takayama2, and John C. Reed2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007976423905
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  • 10
    Electronic Resource
    Electronic Resource
    Growth rate suppression of cultured mammalian cells enhances protein productivity (1994)
    Springer
    Cytotechnology 15 (1994), S. 57-64 
    Details
    ISSN: 1573-0778
    Keywords: Enhancement of protein production ; growth suppression ; cell differentiation ; mammalian cell culture ; mRNA stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Suppression of proliferation of cells which contain stable or stabilized mRNA coded for a protein to be produced, a partial mimic of cell differentiation, was examined for enhancing protein production by cultured mammalian cells. Hybridoma 2E3 cells which were adapted to be interleukin-6 sensitively growth-suppressed accumulated the mRNA of IgG1 which is reported stable, and IgG1 production rate increased as a result when their growth was suppressed with interleukin-6. A myeloma cell line was similarly adapted; the obtained myeloma cells can be used as host cells for enhancing production of exogenous proteins by suppressing growth with interleukin-6. Temperature-sensitively growth-suppressible mutants of mouse mammary carcinoma FM3A were transfected with cDNA of IgM λ1 chain and cultured at nonpermissive temperature to enhance production of λ1. Addition of various growth-suppressive reagents to culture medium was studied for finding methods suitable for suppressing growth while maintaining high cell viability. Caffeine yielded the best results among these reagents. Deprivation of various growth-supporting components in culture medium was also tested; simultaneous deprivation of insulin and transferrin viably suppressed growth of hybridoma 2E3 cells, resulting in enhanced antibody productivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00762379
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