ISSN:
1573-4986
Keywords:
acidic N-glycan
;
zona pellucida
;
sulfation
;
fucosylation
;
ZP, zona pellucida
;
AAL, Aleuria aurantia lectin
;
Con A, concanavalin A
;
aMan, anhydromannose
;
PVL, Psathyrella velutina lectin
;
UEA, Ulex europaeus agglutinin
;
SSA, Sambucus sieboidiana agglutinin
;
Lea, Lewis a
;
Lex, Lewis X. Nomenclature for the ZP glycoproteinsused here is adapted from that proposed by Harris et al.[16] and discussed in greater detail by Hedrick, J. L. [47].Terms used for pig ZP glycoproteins in this paper andequivalent terms used in past publications from ourlaboratory and for the mouse ZP glycoproteins are thefollowing: ZPA=90 K (pig), ZP2 (mouse)
;
ZPB=55 Kα (pig)
;
ZPC=55 Kβ (pig), ZP3 (mouse). Subscript OTindicates NaB3H4-reduced glycans.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Structures of acidic N-glycans released from porcine zona pellucida glycoproteins by hydrazinolysis were studied. The results indicated that the acidic glycans are of mono- to tetraantennary complex-type with and without N-acetyllactosamine repeating units. Sulfated residues are not only located at the C-6 position of GlcNAc included in the N-acetyllactosamine repeating units, but also at the C-6 position of GlcNAc in the non-repeated antennae and at the C-3 position of reducing terminal GlcNAc residue. Analysis of the oligosaccharide fragments released by endo-β-galactosidase digestion and by hydrazine/nitrous acid treatment also revealed that various sulfated and non-sulfated forms of fucosylated structures such as Fucα1→2Galβ1→4(±SO−3→6)GlcNAc (type 2H), Galβ1→4(Fucα1→3)(±SO−3→6)GlcNAc(Lex) and Fucα1→3 or 4(±SO−3→6)GlcNAc, are expressed in the repeated outer chain moieties.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006926801717
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